Restriction map for
pFN21AE1891
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BlnI BpuAI BsaAI BsaBI
BsgI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FseI
FspI HincII HindII HpaI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 505 AAATTT [GA]^AATT[CT]
AcyI 1044 GGCGCC G[GA]^CG[CT]C
AlwNI 252 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 505 AAATTT [GA]^AATT[CT]
BbeI 1044 GGCGCC GGCGC^C
BclI 927 TGATCA T^GATCA
BglI 1174 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 682 AGATCT A^GATCT
BlpI 309 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 309 GCTGAGC GC^T[ACGT]AGC
BsaHI 1044 GGCGCC G[GA]^CG[CT]C
BseRI 229 GAGGAG GAGGAG(10/8)
BsiEI 37 CGACCG CG[GA][CT]^CG
BsmI 615 GCATTC GAATGC(1/-1)
BsrBI 223 CCGCTC CCGCTC(-3/-3)
BsrFI 1047 GCCGGC [GA]^CCGG[CT]
BssHII 153 GCGCGC G^CGCGC
BstAPI 615 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1198 TTCGAA TT^CGAA
CelII 309 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1047 GCCGGC [GA]^CCGG[CT]
DraII 65 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 841 GACACTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 492 CCTCATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 65 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 514 GATATC GAT^ATC
FbaI 927 TGATCA T^GATCA
Hin1I 1044 GGCGCC G[GA]^CG[CT]C
HindIII 287 AAGCTT A^AGCTT
KasI 1044 GGCGCC G^GCGCC
NaeI 1047 GCCGGC GCC^GGC
NarI 1044 GGCGCC GG^CGCC
NgoMIV 1047 GCCGGC G^CCGGC
NspV 1198 TTCGAA TT^CGAA
PaeR7I 226 CTCGAG C^TCGAG
PflMI 251 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 65 GGGACCT [GA]G^G[AT]CC[CT]
SfoI 1044 GGCGCC GGC^GCC
SfuI 1198 TTCGAA TT^CGAA
SmaI 295 CCCGGG CCC^GGG
Van91I 251 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 444 TCTAGA T^CTAGA
XcmI 722 CCAAAGGCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 226 CTCGAG C^TCGAG
XmaCI 295 CCCGGG C^CCGGG
XmaI 295 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 130 TCCGGA T^CCGGA
782 TCCGGA
AlwI 366 GATCC GGATC(4/5)
1137 GATCC
AspHI 754 GTGCTC G[AT]GC[AT]^C
1113 GAGCAC
BanI 180 GGCACC G^G[CT][GA]CC
1044 GGCGCC
BanII 105 GAGCCC G[GA]GC[CT]^C
745 GAGCCC
BfaI 445 CTAG C^TAG
905 CTAG
BpmI 410 CTGGAG CTGGAG(16/14)
1090 CTGGAG
BsaI 15 GAGACC GGTCTC(1/5)
1072 GAGACC
BseAI 130 TCCGGA T^CCGGA
782 TCCGGA
BsiHKAI 754 GTGCTC G[AT]GC[AT]^C
1113 GAGCAC
BsmAI 15 GAGAC GTCTC(1/5)
1072 GAGAC
BspEI 130 TCCGGA T^CCGGA
782 TCCGGA
BspMI 830 ACCTGC ACCTGC(4/8)
48 GCAGGT
BstYI 365 AGATCC [GA]^GATC[CT]
682 AGATCT
DsaI 912 CCATGG C^C[GA][CT]GG
1175 CCATGG
EaeI 1106 TGGCCG [CT]^GGCC[GA]
1172 CGGCCA
EarI 262 GAAGAG CTCTTC(1/4)
961 GAAGAG
EcoT14I 912 CCATGG C^C[AT][AT]GG
1175 CCATGG
HgaI 73 GACGC GACGC(5/10)
397 GACGC
Ksp632I 262 GAAGAG CTCTTC(1/4)
961 GAAGAG
MaeI 445 CTAG C^TAG
905 CTAG
MflI 365 AGATCC [GA]^GATC[CT]
682 AGATCT
MroI 130 TCCGGA T^CCGGA
782 TCCGGA
MseI 497 TTAA T^TAA
584 TTAA
MslI 495 CATTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
949 CACAAGTATG
NcoI 912 CCATGG C^CATGG
1175 CCATGG
StyI 912 CCATGG C^C[AT][AT]GG
1175 CCATGG
Tru9I 497 TTAA T^TAA
584 TTAA
XhoII 365 AGATCC [GA]^GATC[CT]
682 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BbvI BcnI BmyI BsaJI BsaWI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I