Restriction map for
pFN21AE1625
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# NO CUT ENZYMES ( 162 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseAI BseRI BsgI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI Eam1105I EarI Eco47III Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 163 GACGTC GACGT^C
AcsI 318 GAATTT [GA]^AATT[CT]
Alw44I 153 GTGCAC G^TGCAC
AlwI 242 GGATC GGATC(4/5)
ApaLI 153 GTGCAC G^TGCAC
ApoI 318 GAATTT [GA]^AATT[CT]
AspHI 153 GTGCAC G[AT]GC[AT]^C
AvaII 218 GGTCC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BbrPI 237 CACGTG CAC^GTG
BclI 338 TGATCA T^GATCA
BmyI 153 GTGCAC G[AGT]GC[ACT]^C
BpmI 358 CTGGAG CTGGAG(16/14)
BsaAI 237 CACGTG [CT]AC^GT[GA]
BsaWI 167 TCCGGT [AT]^CCGG[AT]
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsiHKAI 153 GTGCAC G[AT]GC[AT]^C
Bsp1286I 153 GTGCAC G[AGT]GC[ACT]^C
BsrBI 125 GAGCGG CCGCTC(-3/-3)
BssHII 270 GCGCGC G^CGCGC
Cfr13I 218 GGTCC G^G[ACGT]CC
DdeI 326 CTAAG C^T[ACGT]AG
DraI 85 TTTAAA TTT^AAA
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoNI 297 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 338 TGATCA T^GATCA
HgaI 412 GACGC GACGC(5/10)
MaeIII 79 GTGAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 86 TTAA T^TAA
MslI 72 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 258 CAGCGG C[AC]G^C[GT]G
NlaIII 207 CATG CATG^
PmaCI 237 CACGTG CAC^GTG
PmlI 237 CACGTG CAC^GTG
PstI 265 CTGCAG CTGCA^G
Sau96I 218 GGTCC G^G[ACGT]CC
SexAI 44 ACCTGGT A^CC[AT]GGT
SfaNI 202 GATGC GCATC(5/9)
SfcI 265 CTGCAG C^T[GA][CT]AG
TaqI 14 TCGA T^CGA
Tru9I 86 TTAA T^TAA
Tsp45I 79 GTGAC ^GT[GC]AC
TthHB8I 14 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 52 CGCG CG^CG
271 CGCG
AcyI 163 GACGTC G[GA]^CG[CT]C
412 GACGCC
AluI 356 AGCT AG^CT
383 AGCT
BbvI 267 GCAGC GCAGC(8/12)
264 GCTGC
BsaHI 163 GACGTC G[GA]^CG[CT]C
412 GACGCC
BsmFI 283 GGGAC GGGAC(10/14)
109 GTCCC
BstF5I 144 CATCC GGATG(2/0)
201 GGATG
BstNI 45 CCTGG CC^[AT]GG
297 CCTGG
BstUI 52 CGCG CG^CG
271 CGCG
EaeI 2 TGGCCA [CT]^GGCC[GA]
65 CGGCCG
EcoRII 45 CCTGG ^CC[AT]GG
297 CCTGG
FauI 260 GCGGG CCCGC(4/6)
394 GCGGG
FokI 144 CATCC GGATG(9/13)
201 GGATG
HaeIII 3 GGCC GG^CC
66 GGCC
Hin1I 163 GACGTC G[GA]^CG[CT]C
412 GACGCC
MboII 364 GAAGA GAAGA(8/7)
387 GAAGA
MvaI 45 CCTGG CC^[AT]GG
297 CCTGG
MvnI 52 CGCG CG^CG
271 CGCG
NlaIV 97 GGCTCC GG[ACGT]^[ACGT]CC
400 GGAGCC
TseI 264 GCTGC G^C[AT]GC
267 GCAGC
Tsp509I 319 AATT ^AATT
406 AATT
TspRI 151 CAGTG CA[GC]TG[ACGT][ACGT]^
342 CAGTG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI BcnI BsaJI BsiYI
BslI BssKI Cac8I CfoI DpnII
Fnu4HI HapII HhaI HinP1I HpaII
HphI ItaI MaeII MboI MnlI
MspI MwoI NciI NdeII RsaI
Sau3AI ScrFI