Restriction map for
pFN21AE9250
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbsI BcgI BclI BcnI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflII 601 CTTAAG C^TTAAG
AlwNI 529 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1002 CCCGAG C^[CT]CG[GA]G
BamHI 950 GGATCC G^GATCC
BbvCI 501 GCTGAGG CCTCAGC(-5/-2)
BciVI 1047 GTATCC GTATCC(6/5)
BfrI 601 CTTAAG C^TTAAG
BsaAI 451 TACGTG [CT]AC^GT[GA]
BsiWI 476 CGTACG C^GTACG
BsmAI 7 GAGAC GTCTC(1/5)
BsmI 384 GAATGC GAATGC(1/-1)
BsoBI 1002 CCCGAG C^[CT]CG[GA]G
BspHI 714 TCATGA T^CATGA
BsrI 435 CCAGT ACTGG(1/-1)
BstBI 398 TTCGAA TT^CGAA
BstYI 950 GGATCC [GA]^GATC[CT]
EcoT14I 678 CCTTGG C^C[AT][AT]GG
HaeII 531 GGCGCT [GA]GCGC^[CT]
HaeIII 78 GGCC GG^CC
HincII 286 GTTGAC GT[CT]^[GA]AC
HindII 286 GTTGAC GT[CT]^[GA]AC
HindIII 808 AAGCTT A^AGCTT
HphI 277 GGTGA GGTGA(8/7)
MaeIII 355 GTGAC ^GT[ACGT]AC
MfeI 539 CAATTG C^AATTG
MflI 950 GGATCC [GA]^GATC[CT]
MunI 539 CAATTG C^AATTG
MwoI 928 GCACCAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 544 GCTAGC G^CTAGC
NspV 398 TTCGAA TT^CGAA
PstI 106 CTGCAG CTGCA^G
PvuII 1008 CAGCTG CAG^CTG
RcaI 714 TCATGA T^CATGA
SexAI 273 ACCAGGT A^CC[AT]GGT
SfuI 398 TTCGAA TT^CGAA
StuI 77 AGGCCT AGG^CCT
StyI 678 CCTTGG C^C[AT][AT]GG
TaqI 399 TCGA T^CGA
Tsp45I 355 GTGAC ^GT[GC]AC
TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 399 TCGA T^CGA
XhoII 950 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 517 CCGC CCGC(-3/-1)
934 GCGG
BseRI 492 CTCCTC GAGGAG(10/8)
379 GAGGAG
BsiYI 497 CCATGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
517 CCGCTTCTGGG
BslI 497 CCATGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
517 CCGCTTCTGGG
BsmFI 991 GGGAC GGGAC(10/14)
1039 GTCCC
BstF5I 3 GGATG GGATG(2/0)
249 GGATG
CfoI 507 GCGC GCG^C
532 GCGC
DraII 991 GGGACCC [GA]G^G[ACGT]CC[CT]
998 AGGACCC
EcoO109I 991 GGGACCC [GA]G^G[ACGT]CC[CT]
998 AGGACCC
FokI 3 GGATG GGATG(9/13)
249 GGATG
HhaI 507 GCGC GCG^C
532 GCGC
HinP1I 507 GCGC G^CGC
532 GCGC
MaeII 452 ACGT A^CGT
487 ACGT
MslI 160 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
213 CATTTATATG
MspA1I 932 CAGCGG C[AC]G^C[GT]G
1008 CAGCTG
PpuMI 991 GGGACCC [GA]G^G[AT]CC[CT]
998 AGGACCC
SmlI 601 CTTAAG C^T[CT][GA]AG
755 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BsaJI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraI EcoRII Fnu4HI HinfI ItaI
MaeI MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp509I