##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiHKAI     BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SbfI        ScaI        SfiI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AlwI            9  GGATC                GGATC(4/5)
AlwNI         769  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         591  GGACC                G^G[AT]CC
BbvCI         552  CCTCAGC              CCTCAGC(-5/-2)
BlpI          135  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      135  GCTGAGC              GC^T[ACGT]AGC
BsiWI         720  CGTACG               C^GTACG
BstF5I         21  CATCC                GGATG(2/0)
CelII         135  GCTGAGC              GC^T[ACGT]AGC
DpnII          10  GATC                 ^GATC
EarI          610  GAAGAG               CTCTTC(1/4)
FokI           21  CATCC                GGATG(9/13)
HgaI          511  GACGC                GACGC(5/10)
Ksp632I       610  GAAGAG               CTCTTC(1/4)
KspI           24  CCGCGG               CCGC^GG
MboI           10  GATC                 ^GATC
NdeII          10  GATC                 ^GATC
NlaIII        645  CATG                 CATG^
PvuII          52  CAGCTG               CAG^CTG
SacII          24  CCGCGG               CCGC^GG
SapI          610  GAAGAGC              GCTCTTC(1/4)
Sau3AI         10  GATC                 ^GATC
SexAI         533  ACCTGGT              A^CC[AT]GGT
SfaNI          20  GCATC                GCATC(5/9)
SgrAI         322  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          339  CCCGGG               CCC^GGG
XcmI          578  CCACCTGGCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         339  CCCGGG               C^CCGGG
XmaI          339  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

ApaI          201  GGGCCC               GGGCC^C
              568  GGGCCC              
DdeI          136  CTGAG                C^T[ACGT]AG
              553  CTCAG               
DraII         260  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              567  GGGGCCC             
DsaI           24  CCGCGG               C^C[GA][CT]GG
              635  CCGTGG              
EcoO109I      260  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              567  GGGGCCC             
EcoT14I       146  CCAAGG               C^C[AT][AT]GG
              179  CCAAGG              
HinfI          37  GAATC                G^A[ACGT]TC
              657  GAATC               
MboII          34  GAAGA                GAAGA(8/7)
              610  GAAGA               
MslI          177  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              645  CATGCCTGTG          
PstI          376  CTGCAG               CTGCA^G
              763  CTGCAG              
SfcI          376  CTGCAG               C^T[GA][CT]AG
              763  CTGCAG              
StyI          146  CCAAGG               C^C[AT][AT]GG
              179  CCAAGG              
TfiI           37  GAATC                G^A[AT]TC
              657  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiEI       BsiYI       BslI        
BsoBI       Bsp1286I    BsrFI       BssHII      BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      EaeI        EagI        EclXI       Eco52I      
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        KasI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NgoMIV      NlaIV       PaeR7I      
RsaI        Sau96I      ScrFI       SfoI        SmlI        
TaqI        TseI        TthHB8I     XhoI