Restriction map for
pFN21AE1940
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII KpnI KspI MaeI MamI
MfeI MluI MroI MslI MunI
MvnI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 769 GTAGAC GT^[AC][GT]AC
AcsI 1069 AAATTC [GA]^AATT[CT]
AflIII 1091 ACGTGT A^C[GA][CT]GT
ApoI 1069 AAATTC [GA]^AATT[CT]
Asp700I 1147 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1307 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 326 TGCGCA TGC^GCA
BamHI 1024 GGATCC G^GATCC
BglI 374 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1263 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1263 GCTGAGC GC^T[ACGT]AGC
BsaAI 599 TACGTA [CT]AC^GT[GA]
BseRI 1117 GAGGAG GAGGAG(10/8)
BsrBI 250 CCGCTC CCGCTC(-3/-3)
BsrFI 1063 GCCGGC [GA]^CCGG[CT]
BstAPI 1268 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1369 CCAGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1024 GGATCC [GA]^GATC[CT]
CelII 1263 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1063 GCCGGC [GA]^CCGG[CT]
DsaI 944 CCATGG C^C[GA][CT]GG
EarI 1147 GAAGAG CTCTTC(1/4)
FspI 326 TGCGCA TGC^GCA
HincII 319 GTTAAC GT[CT]^[GA]AC
HindII 319 GTTAAC GT[CT]^[GA]AC
HpaI 319 GTTAAC GTT^AAC
Ksp632I 1147 GAAGAG CTCTTC(1/4)
MflI 1024 GGATCC [GA]^GATC[CT]
MseI 320 TTAA T^TAA
NaeI 1063 GCCGGC GCC^GGC
NcoI 944 CCATGG C^CATGG
NgoMIV 1063 GCCGGC G^CCGGC
PaeR7I 16 CTCGAG C^TCGAG
PflFI 1307 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 898 CTGCAG CTGCA^G
PvuII 1261 CAGCTG CAG^CTG
SacI 669 GAGCTC GAGCT^C
SapI 1147 GAAGAGC GCTCTTC(1/4)
SmaI 854 CCCGGG CCC^GGG
SmlI 16 CTCGAG C^T[CT][GA]AG
SnaBI 599 TACGTA TAC^GTA
TfiI 1341 GATTC G^A[AT]TC
Tru9I 320 TTAA T^TAA
Tth111I 1307 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 16 CTCGAG C^TCGAG
XhoII 1024 GGATCC [GA]^GATC[CT]
XmaCI 854 CCCGGG C^CCGGG
XmaI 854 CCCGGG C^CCGGG
XmnI 1147 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 602 GTAC GT^AC
1207 GTAC
AlwI 1025 GATCC GGATC(4/5)
1024 GGATC
AocI 285 CCTAAGG CC^T[ACGT]AGG
1075 CCTCAGG
ApaI 230 GGGCCC GGGCC^C
806 GGGCCC
AspHI 669 GAGCTC G[AT]GC[AT]^C
872 GTGCTC
AvaI 16 CTCGAG C^[CT]CG[GA]G
854 CCCGGG
BalI 567 TGGCCA TGG^CCA
1427 TGGCCA
BbeI 139 GGCGCC GGCGC^C
382 GGCGCC
BbsI 1087 GAAGAC GAAGAC(2/6)
1186 GTCTTC
BpuAI 1087 GAAGAC GAAGAC(2/6)
1186 GTCTTC
BsiHKAI 669 GAGCTC G[AT]GC[AT]^C
872 GTGCTC
BsoBI 16 CTCGAG C^[CT]CG[GA]G
854 CCCGGG
BssSI 253 CTCGTG CACGAG(-5/-1)
376 CTCGTG
Bsu36I 285 CCTAAGG CC^T[ACGT]AGG
1075 CCTCAGG
Eco81I 285 CCTAAGG CC^T[ACGT]AGG
1075 CCTCAGG
EcoNI 285 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1110 CCTGATAGAGG
EcoT14I 944 CCATGG C^C[AT][AT]GG
1235 CCTTGG
HaeII 139 GGCGCC [GA]GCGC^[CT]
382 GGCGCC
KasI 139 GGCGCC G^GCGCC
382 GGCGCC
MluNI 567 TGGCCA TGG^CCA
1427 TGGCCA
MscI 567 TGGCCA TGG^CCA
1427 TGGCCA
MspA1I 708 CAGCGG C[AC]G^C[GT]G
1261 CAGCTG
NarI 139 GGCGCC GG^CGCC
382 GGCGCC
NspI 473 GCATGT [GA]CATG^[CT]
611 ACATGC
PflMI 570 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1346 CCATACACTGG
RsaI 602 GTAC GT^AC
1207 GTAC
SfcI 310 CTACAG C^T[GA][CT]AG
898 CTGCAG
SfoI 139 GGCGCC GGC^GCC
382 GGCGCC
StyI 944 CCATGG C^C[AT][AT]GG
1235 CCTTGG
Tsp45I 505 GTGAC ^GT[GC]AC
819 GTCAC
Van91I 570 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1346 CCATACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp509I
TspRI TthHB8I