Restriction map for
pFN21AE6265
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BpmI BpuAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BstAPI BstBI BstEII BstPI
BstXI BstYI Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MboII MfeI MflI MluI MluNI
MscI MseI MunI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StyI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 738 GTATAC GT^[AC][GT]AC
AccII 724 CGCG CG^CG
AccIII 640 TCCGGA T^CCGGA
AflIII 460 ACGTGT A^C[GA][CT]GT
AlwI 767 GGATC GGATC(4/5)
BbrPI 459 CACGTG CAC^GTG
BfaI 332 CTAG C^TAG
BglI 485 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 9 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 9 GCTCAGC GC^T[ACGT]AGC
BsaAI 459 CACGTG [CT]AC^GT[GA]
BsaWI 640 TCCGGA [AT]^CCGG[AT]
BseAI 640 TCCGGA T^CCGGA
BsgI 602 GTGCAG GTGCAG(16/14)
BsmBI 309 CGTCTC CGTCTC(1/5)
BsmFI 147 GTCCC GGGAC(10/14)
BspEI 640 TCCGGA T^CCGGA
BssHII 723 GCGCGC G^CGCGC
BssSI 624 CTCGTG CACGAG(-5/-1)
Bst1107I 738 GTATAC GTA^TAC
BstF5I 428 CATCC GGATG(2/0)
BstUI 724 CGCG CG^CG
BstZ17I 738 GTATAC GTA^TAC
CelII 9 GCTCAGC GC^T[ACGT]AGC
DraII 645 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 240 TGGCCG [CT]^GGCC[GA]
EcoO109I 645 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 428 CATCC GGATG(9/13)
HgaI 366 GACGC GACGC(5/10)
HincII 103 GTCAAC GT[CT]^[GA]AC
HindII 103 GTCAAC GT[CT]^[GA]AC
MaeI 332 CTAG C^TAG
MroI 640 TCCGGA T^CCGGA
MspA1I 162 CAGCGG C[AC]G^C[GT]G
MvnI 724 CGCG CG^CG
NaeI 560 GCCGGC GCC^GGC
NgoMIV 560 GCCGGC G^CCGGC
PaeR7I 743 CTCGAG C^TCGAG
PflMI 470 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 459 CACGTG CAC^GTG
PmlI 459 CACGTG CAC^GTG
SbfI 150 CCTGCAGG CCTGCA^GG
ScaI 566 AGTACT AGT^ACT
SexAI 773 ACCAGGT A^CC[AT]GGT
Sse8387I 150 CCTGCAGG CCTGCA^GG
StuI 453 AGGCCT AGG^CCT
TaqI 744 TCGA T^CGA
TfiI 552 GAATC G^A[AT]TC
TthHB8I 744 TCGA T^CGA
Van91I 470 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 171 CCACTTCTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 743 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 396 GTAC GT^AC
567 GTAC
AlwNI 298 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
759 CAGCACCTG
AvaI 314 CTCGGG C^[CT]CG[GA]G
743 CTCGAG
BanI 616 GGTGCC G^G[CT][GA]CC
703 GGCACC
BsmAI 310 GTCTC GTCTC(1/5)
694 GTCTC
BsmI 114 GAATGC GAATGC(1/-1)
59 GCATTC
BsoBI 314 CTCGGG C^[CT]CG[GA]G
743 CTCGAG
HphI 634 GGTGA GGTGA(8/7)
467 TCACC
MaeII 362 ACGT A^CGT
460 ACGT
MslI 223 CACTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
672 CACATGGGTG
PleI 589 GACTC GAGTC(4/5)
637 GACTC
PstI 151 CTGCAG CTGCA^G
520 CTGCAG
RsaI 396 GTAC GT^AC
567 GTAC
SfaNI 407 GCATC GCATC(5/9)
427 GCATC
SfcI 151 CTGCAG C^T[GA][CT]AG
520 CTGCAG
SmlI 576 CTCAAG C^T[CT][GA]AG
743 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI