Restriction map for
pFN21AE1587
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI KasI Ksp632I KspI
MaeI MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 149 GGTACC G^GTACC
Asp718I 149 GGTACC G^GTACC
AvaI 71 CTCGAG C^[CT]CG[GA]G
BanI 149 GGTACC G^G[CT][GA]CC
BbsI 141 GAAGAC GAAGAC(2/6)
BcnI 90 CCGGG CC^[GC]GG
BpuAI 141 GAAGAC GAAGAC(2/6)
BseRI 34 CTCCTC GAGGAG(10/8)
BsgI 199 GTGCAG GTGCAG(16/14)
BsmFI 76 GGGAC GGGAC(10/14)
BsmI 246 GAATGC GAATGC(1/-1)
BsoBI 71 CTCGAG C^[CT]CG[GA]G
BspMI 260 ACCTGC ACCTGC(4/8)
BstEII 27 GGTCACC G^GT[ACGT]ACC
BstPI 27 GGTCACC G^GT[ACGT]ACC
Cac8I 18 GCTGGC GC[ACGT]^[ACGT]GC
EcoO65I 27 GGTCACC G^GT[ACGT]ACC
HapII 90 CCGG C^CGG
HpaII 90 CCGG C^CGG
HphI 29 TCACC GGTGA(8/7)
KpnI 149 GGTACC GGTAC^C
MaeII 145 ACGT A^CGT
MseI 254 TTAA T^TAA
MspI 90 CCGG C^CGG
MwoI 89 GCCGGGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 90 CCGGG CC^[GC]GG
PaeR7I 71 CTCGAG C^TCGAG
PstI 52 CTGCAG CTGCA^G
ScaI 107 AGTACT AGT^ACT
SfcI 52 CTGCAG C^T[GA][CT]AG
TaqI 72 TCGA T^CGA
Tru9I 254 TTAA T^TAA
Tsp45I 28 GTCAC ^GT[GC]AC
TspRI 8 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 72 TCGA T^CGA
XhoI 71 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 108 GTAC GT^AC
150 GTAC
AluI 69 AGCT AG^CT
137 AGCT
AvaII 77 GGACC G^G[AT]CC
222 GGACC
BpmI 171 CTCCAG CTGGAG(16/14)
103 CTGGAG
BsiYI 33 CCTCCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
36 CCTCCTGGGGG
BslI 33 CCTCCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
36 CCTCCTGGGGG
BstF5I 60 CATCC GGATG(2/0)
177 GGATG
DdeI 130 CTTAG C^T[ACGT]AG
161 CTGAG
FokI 60 CATCC GGATG(9/13)
177 GGATG
MaeIII 28 GTCAC ^GT[ACGT]AC
193 GTAAC
RsaI 108 GTAC GT^AC
150 GTAC
SfaNI 15 GATGC GCATC(5/9)
178 GATGC
SmlI 71 CTCGAG C^T[CT][GA]AG
271 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BbvI BsaJI BssKI BstNI Cfr13I
EcoRII Fnu4HI HaeIII ItaI MboII
MnlI MvaI NlaIV Sau96I ScrFI
TseI