Restriction map for
pFN21AE4010
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DpnII DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 4 GACGCC G[GA]^CG[CT]C
AfaI 141 GTAC GT^AC
AflII 414 CTTAAG C^TTAAG
AlwNI 238 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 157 GAAGAC GAAGAC(2/6)
BfrI 414 CTTAAG C^TTAAG
BpmI 148 CTGGAG CTGGAG(16/14)
BpuAI 157 GAAGAC GAAGAC(2/6)
BsaHI 4 GACGCC G[GA]^CG[CT]C
BsaJI 402 CCGAGG C^C[ACGT][ACGT]GG
BseRI 39 CTCCTC GAGGAG(10/8)
BsiYI 402 CCGAGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 402 CCGAGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssKI 147 CCTGG ^CC[ACGT]GG
BstNI 147 CCTGG CC^[AT]GG
Cac8I 462 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 28 GCGC GCG^C
DraI 361 TTTAAA TTT^AAA
EarI 166 GAAGAG CTCTTC(1/4)
EcoRII 147 CCTGG ^CC[AT]GG
HaeIII 400 GGCC GG^CC
HgaI 4 GACGC GACGC(5/10)
HhaI 28 GCGC GCG^C
Hin1I 4 GACGCC G[GA]^CG[CT]C
HinP1I 28 GCGC G^CGC
HinfI 252 GATTC G^A[ACGT]TC
Ksp632I 166 GAAGAG CTCTTC(1/4)
MvaI 147 CCTGG CC^[AT]GG
NspI 329 ACATGC [GA]CATG^[CT]
PstI 244 CTGCAG CTGCA^G
PvuII 238 CAGCTG CAG^CTG
RsaI 141 GTAC GT^AC
ScaI 140 AGTACT AGT^ACT
ScrFI 147 CCTGG CC^[ACGT]GG
SmlI 414 CTTAAG C^T[CT][GA]AG
TaqI 326 TCGA T^CGA
TfiI 252 GATTC G^A[AT]TC
TspRI 420 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 326 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 58 GAATTT [GA]^AATT[CT]
126 AAATTT
ApoI 58 GAATTT [GA]^AATT[CT]
126 AAATTT
BsrI 408 ACTGG ACTGG(1/-1)
111 CCAGT
BstF5I 99 GGATG GGATG(2/0)
303 GGATG
FokI 99 GGATG GGATG(9/13)
303 GGATG
MaeIII 317 GTGAC ^GT[ACGT]AC
433 GTGAC
MslI 90 CATTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
298 CATAAGGATG
MspA1I 238 CAGCTG C[AC]G^C[GT]G
247 CAGCGG
MwoI 46 GCCTCTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
240 GCTGCTGCAGC
SfaNI 52 GATGC GCATC(5/9)
64 GATGC
SfcI 244 CTGCAG C^T[GA][CT]AG
381 CTACAG
Tsp45I 317 GTGAC ^GT[GC]AC
433 GTGAC
Tsp509I 59 AATT ^AATT
127 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AciI AluI BbvI DdeI Fnu4HI
HphI ItaI MboII MnlI MseI
NlaIII Tru9I TseI