Restriction map for
pFN21AB9733
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AviII BamHI
BbrPI BbsI BciVI BclI BfrI
BglII BpuAI BsaBI BseAI BsgI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI DraI
DraIII Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
MamI MfeI MflI MluI MroI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI SalI
ScaI SfiI SfuI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 293 GGTACC G^GTACC
AgeI 1297 ACCGGT A^CCGGT
AlwI 319 GGATC GGATC(4/5)
Asp700I 688 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 293 GGTACC G^GTACC
AspI 1033 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1110 CCTAGG C^CTAGG
BbvCI 738 CCTCAGC CCTCAGC(-5/-2)
BcgI 1370 CGAAAATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1110 CCTAGG C^CTAGG
BlpI 661 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 661 GCTGAGC GC^T[ACGT]AGC
BsaAI 244 TACGTG [CT]AC^GT[GA]
BsaI 1307 GGTCTC GGTCTC(1/5)
BsiWI 242 CGTACG C^GTACG
BssHII 499 GCGCGC G^CGCGC
BssSI 54 CTCGTG CACGAG(-5/-1)
CelII 661 GCTGAGC GC^T[ACGT]AGC
CpoI 1299 CGGTCCG CG^G[AT]CCG
DrdI 1185 GACGGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KpnI 293 GGTACC GGTAC^C
MaeIII 1146 GTCAC ^GT[ACGT]AC
NotI 214 GCGGCCGC GC^GGCCGC
PflFI 1033 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 995 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1297 ACCGGT A^CCGGT
RsrII 1299 CGGTCCG CG^G[AT]CCG
SacI 103 GAGCTC GAGCT^C
SapI 113 GCTCTTC GCTCTTC(1/4)
SbfI 712 CCTGCAGG CCTGCA^GG
SexAI 484 ACCTGGT A^CC[AT]GGT
SfaNI 900 GCATC GCATC(5/9)
Sse8387I 712 CCTGCAGG CCTGCA^GG
StuI 386 AGGCCT AGG^CCT
Tsp45I 1146 GTCAC ^GT[GC]AC
Tsp509I 1424 AATT ^AATT
TspRI 1165 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1033 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 995 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 688 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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BalI 446 TGGCCA TGG^CCA
1266 TGGCCA
BfaI 1111 CTAG C^TAG
1396 CTAG
BglI 37 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
157 GCCTTCACGGC
BpmI 193 CTCCAG CTGGAG(16/14)
586 CTGGAG
BsaWI 347 ACCGGA [AT]^CCGG[AT]
1297 ACCGGT
BsiEI 108 CGGCCG CG[GA][CT]^CG
215 CGGCCG
BsmBI 724 GAGACG CGTCTC(1/5)
760 GAGACG
BsrBI 111 CCGCTC CCGCTC(-3/-3)
1346 GAGCGG
BsrI 195 CCAGT ACTGG(1/-1)
1386 CCAGT
EagI 108 CGGCCG C^GGCCG
215 CGGCCG
EarI 114 CTCTTC CTCTTC(1/4)
565 GAAGAG
EclXI 108 CGGCCG C^GGCCG
215 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
215 CGGCCG
EcoNI 468 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1171 CCTTTTCCAGG
HinfI 745 GACTC G^A[ACGT]TC
1411 GACTC
HphI 455 TCACC GGTGA(8/7)
470 TCACC
Ksp632I 114 CTCTTC CTCTTC(1/4)
565 GAAGAG
MaeI 1111 CTAG C^TAG
1396 CTAG
MaeII 245 ACGT A^CGT
532 ACGT
MluNI 446 TGGCCA TGG^CCA
1266 TGGCCA
MscI 446 TGGCCA TGG^CCA
1266 TGGCCA
MseI 755 TTAA T^TAA
1324 TTAA
PleI 745 GACTC GAGTC(4/5)
1411 GACTC
PstI 713 CTGCAG CTGCA^G
790 CTGCAG
SfcI 713 CTGCAG C^T[GA][CT]AG
790 CTGCAG
SmlI 382 CTCAAG C^T[CT][GA]AG
511 CTCAAG
TaqI 151 TCGA T^CGA
782 TCGA
Tru9I 755 TTAA T^TAA
1324 TTAA
TthHB8I 151 TCGA T^CGA
782 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
KspI MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PpuMI RsaI SacII
Sau3AI Sau96I ScrFI SfoI SgrAI
SmaI StyI TseI XmaCI XmaI