Restriction map for
pFN21AE1899
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbeI
BbsI BbvCI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2134 GTCTAC GT^[AC][GT]AC
AccII 243 CGCG CG^CG
AhdI 1425 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2274 CCTGAGG CC^T[ACGT]AGG
AspEI 1425 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 274 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 216 CACGTG CAC^GTG
BcgI 812 GCAGCCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2332 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 216 CACGTG [CT]AC^GT[GA]
BsaBI 961 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2469 GGTCTC GGTCTC(1/5)
BsgI 1349 GTGCAG GTGCAG(16/14)
Bsp1407I 1734 TGTACA T^GTACA
BsrDI 2399 GCAATG GCAATG(2/0)
BsrGI 1734 TGTACA T^GTACA
BssSI 820 CTCGTG CACGAG(-5/-1)
BstUI 243 CGCG CG^CG
Bsu36I 2274 CCTGAGG CC^T[ACGT]AGG
CfoI 242 GCGC GCG^C
DraIII 654 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2447 CCGTGG C^C[GA][CT]GG
Eam1105I 1425 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2274 CCTGAGG CC^T[ACGT]AGG
EcoNI 48 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1358 CCAAGG C^C[AT][AT]GG
HhaI 242 GCGC GCG^C
HinP1I 242 GCGC G^CGC
HincII 481 GTCAAC GT[CT]^[GA]AC
HindII 481 GTCAAC GT[CT]^[GA]AC
MamI 961 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 156 CAATTG C^AATTG
MseI 773 TTAA T^TAA
MunI 156 CAATTG C^AATTG
MvnI 243 CGCG CG^CG
NheI 1870 GCTAGC G^CTAGC
PflFI 274 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 216 CACGTG CAC^GTG
PmlI 216 CACGTG CAC^GTG
PshAI 2205 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspBI 1734 TGTACA T^GTACA
StuI 1811 AGGCCT AGG^CCT
StyI 1358 CCAAGG C^C[AT][AT]GG
TaqI 686 TCGA T^CGA
Tru9I 773 TTAA T^TAA
Tth111I 274 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 686 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 706 GGCGTC G[GA]^CG[CT]C
1458 GACGCC
AflIII 215 ACACGT A^C[GA][CT]GT
217 ACGTGT
BciVI 221 GTATCC GTATCC(6/5)
438 GTATCC
BfaI 538 CTAG C^TAG
1871 CTAG
BsaHI 706 GGCGTC G[GA]^CG[CT]C
1458 GACGCC
BstAPI 318 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
693 GCACCTCCTGC
BstYI 966 AGATCC [GA]^GATC[CT]
1655 AGATCC
EarI 1882 GAAGAG CTCTTC(1/4)
2361 GAAGAG
FauI 809 CCCGC CCCGC(4/6)
244 GCGGG
Hin1I 706 GGCGTC G[GA]^CG[CT]C
1458 GACGCC
Ksp632I 1882 GAAGAG CTCTTC(1/4)
2361 GAAGAG
MaeI 538 CTAG C^TAG
1871 CTAG
MflI 966 AGATCC [GA]^GATC[CT]
1655 AGATCC
PleI 1285 GAGTC GAGTC(4/5)
1431 GAGTC
PstI 52 CTGCAG CTGCA^G
1691 CTGCAG
SmlI 1818 CTCAAG C^T[CT][GA]AG
2412 CTCAAG
TfiI 518 GAATC G^A[AT]TC
795 GATTC
XhoII 966 AGATCC [GA]^GATC[CT]
1655 AGATCC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I Tsp509I TspRI XcmI