################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 13 GAATTT [GA]^AATT[CT] AluI 332 AGCT AG^CT AlwI 665 GGATC GGATC(4/5) AocI 379 CCTGAGG CC^T[ACGT]AGG ApoI 13 GAATTT [GA]^AATT[CT] AvaII 628 GGACC G^G[AT]CC BpmI 596 CTGGAG CTGGAG(16/14) BsaI 324 GGTCTC GGTCTC(1/5) BsgI 85 GTGCAG GTGCAG(16/14) BsmAI 325 GTCTC GTCTC(1/5) BsmI 396 GAATGC GAATGC(1/-1) BsrDI 20 GCAATG GCAATG(2/0) BsrFI 183 GCCGGC [GA]^CCGG[CT] BssSI 214 CTCGTG CACGAG(-5/-1) BstYI 665 GGATCT [GA]^GATC[CT] Bsu36I 379 CCTGAGG CC^T[ACGT]AGG Cfr10I 183 GCCGGC [GA]^CCGG[CT] DdeI 380 CTGAG C^T[ACGT]AG EarI 121 CTCTTC CTCTTC(1/4) Eco81I 379 CCTGAGG CC^T[ACGT]AGG EcoRV 571 GATATC GAT^ATC HapII 184 CCGG C^CGG HgaI 477 GACGC GACGC(5/10) HpaII 184 CCGG C^CGG Ksp632I 121 CTCTTC CTCTTC(1/4) MflI 665 GGATCT [GA]^GATC[CT] MspA1I 331 CAGCTG C[AC]G^C[GT]G MspI 184 CCGG C^CGG NaeI 183 GCCGGC GCC^GGC NgoMIV 183 GCCGGC G^CCGGC PleI 375 GAGTC GAGTC(4/5) PstI 178 CTGCAG CTGCA^G PvuII 331 CAGCTG CAG^CTG SexAI 299 ACCTGGT A^CC[AT]GGT SfcI 178 CTGCAG C^T[GA][CT]AG StuI 291 AGGCCT AGG^CCT TaqI 101 TCGA T^CGA TfiI 609 GAATC G^A[AT]TC Tsp509I 14 AATT ^AATT TthHB8I 101 TCGA T^CGA XhoII 665 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 72 GTAC GT^AC 318 GTAC AvaI 337 CTCGGG C^[CT]CG[GA]G 349 CTCGGG BalI 362 TGGCCA TGG^CCA 641 TGGCCA BanI 137 GGTGCC G^G[CT][GA]CC 505 GGTGCC BbsI 519 GAAGAC GAAGAC(2/6) 738 GAAGAC BpuAI 519 GAAGAC GAAGAC(2/6) 738 GAAGAC BseRI 310 CTCCTC GAGGAG(10/8) 382 GAGGAG BsmFI 160 GGGAC GGGAC(10/14) 267 GTCCC BsoBI 337 CTCGGG C^[CT]CG[GA]G 349 CTCGGG DsaI 195 CCACGG C^C[GA][CT]GG 566 CCGTGG EaeI 362 TGGCCA [CT]^GGCC[GA] 641 TGGCCA EcoT14I 359 CCTTGG C^C[AT][AT]GG 365 CCAAGG EcoT22I 398 ATGCAT ATGCA^T 498 ATGCAT FauI 613 CCCGC CCCGC(4/6) 743 CCCGC HinfI 375 GAGTC G^A[ACGT]TC 609 GAATC MaeIII 418 GTGAC ^GT[ACGT]AC 562 GTCAC MluNI 362 TGGCCA TGG^CCA 641 TGGCCA MscI 362 TGGCCA TGG^CCA 641 TGGCCA NsiI 398 ATGCAT ATGCA^T 498 ATGCAT PflMI 195 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 644 CCAACCACTGG RsaI 72 GTAC GT^AC 318 GTAC StyI 359 CCTTGG C^C[AT][AT]GG 365 CCAAGG Tsp45I 418 GTGAC ^GT[GC]AC 562 GTCAC Van91I 195 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 644 CCAACCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI ApaI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HphI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI