Restriction map for
pFN21AE5420
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsiWI BsmBI BsmI
Bsp1407I BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MluNI
MscI MspA1I MunI MvaI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 411 CGCG CG^CG
AccIII 692 TCCGGA T^CCGGA
AhdI 392 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 297 AGCT AG^CT
Alw44I 589 GTGCAC G^TGCAC
AlwI 728 GATCC GGATC(4/5)
ApaLI 589 GTGCAC G^TGCAC
AspEI 392 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 589 GTGCAC G[AT]GC[AT]^C
BbsI 316 GTCTTC GAAGAC(2/6)
BciVI 20 GTATCC GTATCC(6/5)
BclI 32 TGATCA T^GATCA
BfaI 205 CTAG C^TAG
BpmI 309 CTCCAG CTGGAG(16/14)
BpuAI 316 GTCTTC GAAGAC(2/6)
BsaBI 27 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 692 TCCGGA [AT]^CCGG[AT]
BseAI 692 TCCGGA T^CCGGA
BseRI 835 CTCCTC GAGGAG(10/8)
BsgI 117 GTGCAG GTGCAG(16/14)
BsiEI 846 CGACCG CG[GA][CT]^CG
BsiHKAI 589 GTGCAC G[AT]GC[AT]^C
BspEI 692 TCCGGA T^CCGGA
BspMI 196 ACCTGC ACCTGC(4/8)
BsrDI 75 CATTGC GCAATG(2/0)
BstUI 411 CGCG CG^CG
BstXI 74 CCATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 727 AGATCC [GA]^GATC[CT]
DsaI 824 CCATGG C^C[GA][CT]GG
EaeI 920 CGGCCA [CT]^GGCC[GA]
Eam1105I 392 GACACAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 824 CCATGG C^C[AT][AT]GG
FbaI 32 TGATCA T^GATCA
MaeI 205 CTAG C^TAG
MaeII 398 ACGT A^CGT
MamI 27 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 727 AGATCC [GA]^GATC[CT]
MroI 692 TCCGGA T^CCGGA
MvnI 411 CGCG CG^CG
NcoI 824 CCATGG C^CATGG
NlaIII 825 CATG CATG^
PstI 431 CTGCAG CTGCA^G
StyI 824 CCATGG C^C[AT][AT]GG
TfiI 689 GATTC G^A[AT]TC
XhoII 727 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 355 GGGCTC G[GA]GC[CT]^C
721 GAGCCC
BcgI 220 CGAGGCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
230 GCAAGAAAATCG
BsaI 485 GAGACC GGTCTC(1/5)
734 GAGACC
BspDI 238 ATCGAT AT^CGAT
686 ATCGAT
CfoI 410 GCGC GCG^C
930 GCGC
ClaI 238 ATCGAT AT^CGAT
686 ATCGAT
DdeI 328 CTCAG C^T[ACGT]AG
406 CTCAG
HaeIII 306 GGCC GG^CC
921 GGCC
HhaI 410 GCGC GCG^C
930 GCGC
HinP1I 410 GCGC G^CGC
930 GCGC
MseI 67 TTAA T^TAA
439 TTAA
MslI 75 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
783 CATCTCGATG
PleI 502 GACTC GAGTC(4/5)
901 GAGTC
SmaI 490 CCCGGG CCC^GGG
803 CCCGGG
Tru9I 67 TTAA T^TAA
439 TTAA
Tsp509I 570 AATT ^AATT
877 AATT
XmaCI 490 CCCGGG C^CCGGG
803 CCCGGG
XmaI 490 CCCGGG C^CCGGG
803 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AvaI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I Cac8I Cfr13I DpnII DraII
EcoO109I FauI Fnu4HI FokI HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MwoI
NciI NdeII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I