Restriction map for
pFN21AE3894
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI MamI MfeI MluI MluNI
MroI MscI MslI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 645 GGTACC G^GTACC
AcyI 142 GGCGCC G[GA]^CG[CT]C
AlwI 339 GATCC GGATC(4/5)
Asp718I 645 GGTACC G^GTACC
AvaI 400 CCCGAG C^[CT]CG[GA]G
AvaII 189 GGACC G^G[AT]CC
AviII 507 TGCGCA TGC^GCA
BbeI 142 GGCGCC GGCGC^C
BfaI 703 CTAG C^TAG
BsaHI 142 GGCGCC G[GA]^CG[CT]C
BsaI 355 GGTCTC GGTCTC(1/5)
BsoBI 400 CCCGAG C^[CT]CG[GA]G
Bsp1407I 662 TGTACA T^GTACA
BsrBI 736 GAGCGG CCGCTC(-3/-3)
BsrFI 165 GCCGGC [GA]^CCGG[CT]
BsrGI 662 TGTACA T^GTACA
BssSI 149 CACGAG CACGAG(-5/-1)
BstYI 338 AGATCC [GA]^GATC[CT]
Cfr10I 165 GCCGGC [GA]^CCGG[CT]
DraI 697 TTTAAA TTT^AAA
EarI 529 GAAGAG CTCTTC(1/4)
EcoNI 787 CCTATCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 755 CCATGG C^C[AT][AT]GG
FspI 507 TGCGCA TGC^GCA
HgaI 226 GACGC GACGC(5/10)
Hin1I 142 GGCGCC G[GA]^CG[CT]C
HincII 91 GTCAAC GT[CT]^[GA]AC
HindII 91 GTCAAC GT[CT]^[GA]AC
HphI 692 TCACC GGTGA(8/7)
KasI 142 GGCGCC G^GCGCC
KpnI 645 GGTACC GGTAC^C
Ksp632I 529 GAAGAG CTCTTC(1/4)
KspI 113 CCGCGG CCGC^GG
MaeI 703 CTAG C^TAG
MaeII 293 ACGT A^CGT
MaeIII 430 GTGAC ^GT[ACGT]AC
MflI 338 AGATCC [GA]^GATC[CT]
NaeI 165 GCCGGC GCC^GGC
NarI 142 GGCGCC GG^CGCC
NcoI 755 CCATGG C^CATGG
NgoMIV 165 GCCGGC G^CCGGC
NspI 659 GCATGT [GA]CATG^[CT]
PflMI 810 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 188 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 715 CAGCTG CAG^CTG
SacII 113 CCGCGG CCGC^GG
SfoI 142 GGCGCC GGC^GCC
SspBI 662 TGTACA T^GTACA
StuI 203 AGGCCT AGG^CCT
StyI 755 CCATGG C^C[AT][AT]GG
Tsp45I 430 GTGAC ^GT[GC]AC
Tsp509I 749 AATT ^AATT
Van91I 810 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 338 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanI 142 GGCGCC G^G[CT][GA]CC
645 GGTACC
BbsI 223 GAAGAC GAAGAC(2/6)
802 GAAGAC
BpmI 100 CTGGAG CTGGAG(16/14)
674 CTGGAG
BpuAI 223 GAAGAC GAAGAC(2/6)
802 GAAGAC
BsiEI 8 CGGCCG CG[GA][CT]^CG
128 CGGCCG
BstXI 177 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
443 CCAAGATCATGG
DdeI 71 CTGAG C^T[ACGT]AG
743 CTGAG
EaeI 8 CGGCCG [CT]^GGCC[GA]
128 CGGCCG
EagI 8 CGGCCG C^GGCCG
128 CGGCCG
EclXI 8 CGGCCG C^GGCCG
128 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
128 CGGCCG
HaeII 142 GGCGCC [GA]GCGC^[CT]
632 AGCGCC
MseI 617 TTAA T^TAA
698 TTAA
PstI 160 CTGCAG CTGCA^G
552 CTGCAG
SfcI 160 CTGCAG C^T[GA][CT]AG
552 CTGCAG
TaqI 346 TCGA T^CGA
791 TCGA
Tru9I 617 TTAA T^TAA
698 TTAA
TthHB8I 346 TCGA T^CGA
791 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TseI TspRI XcmI