Restriction map for
pFN21AE5729
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbvCI BciVI BclI BfrI BlnI
BseRI BsgI BsiWI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 614 TCCGGA T^CCGGA
AflIII 59 ACACGT A^C[GA][CT]GT
Alw44I 1080 GTGCAC G^TGCAC
ApaLI 1080 GTGCAC G^TGCAC
AspHI 1080 GTGCAC G[AT]GC[AT]^C
AspI 913 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4 CACGTG CAC^GTG
BcgI 847 GCACTTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1571 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 367 CTGGAG CTGGAG(16/14)
BsaI 1312 GAGACC GGTCTC(1/5)
BseAI 614 TCCGGA T^CCGGA
BsiEI 938 CGGCCG CG[GA][CT]^CG
BsiHKAI 1080 GTGCAC G[AT]GC[AT]^C
BspEI 614 TCCGGA T^CCGGA
BsrBI 1432 GAGCGG CCGCTC(-3/-3)
BstXI 1367 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1017 GACAGAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 938 CGGCCG C^GGCCG
EarI 172 CTCTTC CTCTTC(1/4)
EclXI 938 CGGCCG C^GGCCG
Eco52I 938 CGGCCG C^GGCCG
EcoNI 18 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 874 GAATTC G^AATTC
HindIII 1048 AAGCTT A^AGCTT
Ksp632I 172 CTCTTC CTCTTC(1/4)
MroI 614 TCCGGA T^CCGGA
NheI 298 GCTAGC G^CTAGC
NspI 1325 GCATGT [GA]CATG^[CT]
PflFI 913 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 477 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4 CACGTG CAC^GTG
PmlI 4 CACGTG CAC^GTG
SapI 171 GCTCTTC GCTCTTC(1/4)
SbfI 660 CCTGCAGG CCTGCA^GG
SmlI 222 CTTGAG C^T[CT][GA]AG
Sse8387I 660 CCTGCAGG CCTGCA^GG
Tth111I 913 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 477 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1023 GACGTC GACGT^C
1885 GACGTC
AcsI 874 GAATTC [GA]^AATT[CT]
1501 GAATTT
AcyI 1023 GACGTC G[GA]^CG[CT]C
1885 GACGTC
AlwNI 1822 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1876 CAGCCGCTG
ApoI 874 GAATTC [GA]^AATT[CT]
1501 GAATTT
BanI 687 GGTGCC G^G[CT][GA]CC
1840 GGCACC
BbsI 910 GAAGAC GAAGAC(2/6)
1157 GTCTTC
BfaI 299 CTAG C^TAG
703 CTAG
BglII 545 AGATCT A^GATCT
1284 AGATCT
BpuAI 910 GAAGAC GAAGAC(2/6)
1157 GTCTTC
BsaAI 4 CACGTG [CT]AC^GT[GA]
60 CACGTA
BsaHI 1023 GACGTC G[GA]^CG[CT]C
1885 GACGTC
BsaWI 614 TCCGGA [AT]^CCGG[AT]
773 ACCGGA
BsmBI 240 CGTCTC CGTCTC(1/5)
1021 GAGACG
BspMI 17 ACCTGC ACCTGC(4/8)
1315 ACCTGC
BssSI 961 CACGAG CACGAG(-5/-1)
1039 CTCGTG
BstAPI 33 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1821 GCAGGCCCTGC
BstYI 545 AGATCT [GA]^GATC[CT]
1284 AGATCT
Hin1I 1023 GACGTC G[GA]^CG[CT]C
1885 GACGTC
MaeI 299 CTAG C^TAG
703 CTAG
MflI 545 AGATCT [GA]^GATC[CT]
1284 AGATCT
MseI 520 TTAA T^TAA
1505 TTAA
MspA1I 1690 CAGCGG C[AC]G^C[GT]G
1879 CCGCTG
StuI 1151 AGGCCT AGG^CCT
1394 AGGCCT
Tru9I 520 TTAA T^TAA
1505 TTAA
Tsp45I 283 GTCAC ^GT[GC]AC
1124 GTGAC
XcmI 1652 CCATCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1752 CCACACCAACTTTGG
XhoII 545 AGATCT [GA]^GATC[CT]
1284 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BalI BanII BbvI BcnI
BlpI BmyI Bpu1102I BsaBI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MamI MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp509I
TspRI TthHB8I