Restriction map for
pFN21AE2743
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AccI AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AviII AvrII BfrI BlnI BlpI
Bpu1102I BsiEI BsiWI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SfuI SnaBI
SpeI SphI SspBI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2795 GGTACC G^GTACC
Alw44I 1228 GTGCAC G^TGCAC
Aor51HI 3072 AGCGCT AGC^GCT
ApaI 2498 GGGCCC GGGCC^C
ApaLI 1228 GTGCAC G^TGCAC
Asp700I 1776 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2795 GGTACC G^GTACC
AspI 883 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3003 GGATCC G^GATCC
BbeI 3271 GGCGCC GGCGC^C
BbrPI 184 CACGTG CAC^GTG
BcgI 2019 CGACTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 184 CACGTG [CT]AC^GT[GA]
BsaBI 91 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 147 GAATGC GAATGC(1/-1)
BspLU11I 2958 ACATGT A^CATGT
BsrBI 592 GAGCGG CCGCTC(-3/-3)
BstEII 3204 GGTGACC G^GT[ACGT]ACC
BstPI 3204 GGTGACC G^GT[ACGT]ACC
DraIII 3009 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3072 AGCGCT AGC^GCT
EcoO65I 3204 GGTGACC G^GT[ACGT]ACC
KasI 3271 GGCGCC G^GCGCC
KpnI 2795 GGTACC GGTAC^C
MaeII 185 ACGT A^CGT
MamI 91 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3271 GGCGCC GG^CGCC
NheI 3304 GCTAGC G^CTAGC
PflFI 883 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 184 CACGTG CAC^GTG
PmlI 184 CACGTG CAC^GTG
PshAI 1983 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 261 CCTGCAGG CCTGCA^GG
ScaI 2259 AGTACT AGT^ACT
SexAI 1253 ACCAGGT A^CC[AT]GGT
SfoI 3271 GGCGCC GGC^GCC
SgrAI 3220 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2766 CCCGGG CCC^GGG
Sse8387I 261 CCTGCAGG CCTGCA^GG
Tth111I 883 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2766 CCCGGG C^CCGGG
XmaI 2766 CCCGGG C^CCGGG
XmnI 1776 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccIII 3077 TCCGGA T^CCGGA
3186 TCCGGA
AflIII 185 ACGTGT A^C[GA][CT]GT
2958 ACATGT
BanI 2795 GGTACC G^G[CT][GA]CC
3271 GGCGCC
BclI 1058 TGATCA T^GATCA
1928 TGATCA
BglII 651 AGATCT A^GATCT
2903 AGATCT
BsaWI 3077 TCCGGA [AT]^CCGG[AT]
3186 TCCGGA
BseAI 3077 TCCGGA T^CCGGA
3186 TCCGGA
BseRI 939 CTCCTC GAGGAG(10/8)
1675 GAGGAG
BsmBI 1431 CGTCTC CGTCTC(1/5)
2658 CGTCTC
BspEI 3077 TCCGGA T^CCGGA
3186 TCCGGA
BstAPI 87 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
291 GCAGCGTCTGC
FbaI 1058 TGATCA T^GATCA
1928 TGATCA
KspI 3161 CCGCGG CCGC^GG
3197 CCGCGG
MroI 3077 TCCGGA T^CCGGA
3186 TCCGGA
NspI 2627 ACATGC [GA]CATG^[CT]
2958 ACATGT
SacI 686 GAGCTC GAGCT^C
3350 GAGCTC
SacII 3161 CCGCGG CCGC^GG
3197 CCGCGG
SapI 1941 GAAGAGC GCTCTTC(1/4)
2832 GAAGAGC
SmlI 1037 CTCAAG C^T[CT][GA]AG
2185 CTCAAG
StuI 1379 AGGCCT AGG^CCT
1669 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
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AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII