Restriction map for
pFN21AE3888
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AhdI ApaI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI Ksp632I
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 120 GGTACC G^GTACC
AfaI 121 GTAC GT^AC
AflIII 731 ACATGT A^C[GA][CT]GT
AgeI 548 ACCGGT A^CCGGT
AlwNI 878 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 883 CCTGAGG CC^T[ACGT]AGG
Aor51HI 335 AGCGCT AGC^GCT
Asp700I 729 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 120 GGTACC G^GTACC
BalI 796 TGGCCA TGG^CCA
BanI 120 GGTACC G^G[CT][GA]CC
BanII 67 GAGCCC G[GA]GC[CT]^C
BbsI 255 GTCTTC GAAGAC(2/6)
BbvCI 768 CCTCAGC CCTCAGC(-5/-2)
BglI 184 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 255 GTCTTC GAAGAC(2/6)
BsaI 59 GAGACC GGTCTC(1/5)
BspLU11I 731 ACATGT A^CATGT
BspMI 117 GCAGGT ACCTGC(4/8)
BsrBI 301 GAGCGG CCGCTC(-3/-3)
BsrFI 548 ACCGGT [GA]^CCGG[CT]
BsrI 6 CCAGT ACTGG(1/-1)
BstAPI 457 GCAATAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 297 GGATG GGATG(2/0)
BstYI 540 GGATCT [GA]^GATC[CT]
Bsu36I 883 CCTGAGG CC^T[ACGT]AGG
Cfr10I 548 ACCGGT [GA]^CCGG[CT]
DraII 879 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 796 TGGCCA [CT]^GGCC[GA]
Eco47III 335 AGCGCT AGC^GCT
Eco81I 883 CCTGAGG CC^T[ACGT]AGG
EcoO109I 879 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 148 GATATC GAT^ATC
FauI 303 GCGGG CCCGC(4/6)
FokI 297 GGATG GGATG(9/13)
KpnI 120 GGTACC GGTAC^C
MaeII 208 ACGT A^CGT
MflI 540 GGATCT [GA]^GATC[CT]
MluNI 796 TGGCCA TGG^CCA
MscI 796 TGGCCA TGG^CCA
NspI 731 ACATGT [GA]CATG^[CT]
PflMI 124 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 548 ACCGGT A^CCGGT
PvuII 199 CAGCTG CAG^CTG
RsaI 121 GTAC GT^AC
SfcI 777 CTACAG C^T[GA][CT]AG
SmaI 716 CCCGGG CCC^GGG
SpeI 630 ACTAGT A^CTAGT
Van91I 124 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 540 GGATCT [GA]^GATC[CT]
XmaCI 716 CCCGGG C^CCGGG
XmaI 716 CCCGGG C^CCGGG
XmnI 729 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AciI 303 GCGG CCGC(-3/-1)
591 GCGG
AluI 200 AGCT AG^CT
874 AGCT
Alw44I 488 GTGCAC G^TGCAC
866 GTGCAC
AlwI 540 GGATC GGATC(4/5)
701 GGATC
ApaLI 488 GTGCAC G^TGCAC
866 GTGCAC
AspHI 488 GTGCAC G[AT]GC[AT]^C
866 GTGCAC
AvaII 164 GGACC G^G[AT]CC
663 GGACC
BcnI 716 CCCGG CC^[GC]GG
717 CCGGG
BfaI 500 CTAG C^TAG
631 CTAG
BsaWI 548 ACCGGT [AT]^CCGG[AT]
654 TCCGGT
BsgI 98 GTGCAG GTGCAG(16/14)
464 GTGCAG
BsiHKAI 488 GTGCAC G[AT]GC[AT]^C
866 GTGCAC
BsiYI 105 CCACCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
124 CCACCCTGTGG
BslI 105 CCACCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
124 CCACCCTGTGG
BsmAI 59 GAGAC GTCTC(1/5)
803 GTCTC
BsrDI 615 CATTGC GCAATG(2/0)
344 GCAATG
CfoI 288 GCGC GCG^C
336 GCGC
DpnII 541 GATC ^GATC
702 GATC
DraIII 15 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
125 CACCCTGTG
HaeII 287 GGCGCT [GA]GCGC^[CT]
335 AGCGCT
HhaI 288 GCGC GCG^C
336 GCGC
HinP1I 288 GCGC G^CGC
336 GCGC
HincII 157 GTCAAC GT[CT]^[GA]AC
358 GTTGAC
HindII 157 GTCAAC GT[CT]^[GA]AC
358 GTTGAC
MaeI 500 CTAG C^TAG
631 CTAG
MboI 541 GATC ^GATC
702 GATC
MseI 508 TTAA T^TAA
740 TTAA
MslI 747 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
813 CACCTTTGTG
MspA1I 199 CAGCTG C[AC]G^C[GT]G
589 CAGCGG
NciI 716 CCCGG CC^[GC]GG
717 CCGGG
NdeII 541 GATC ^GATC
702 GATC
Sau3AI 541 GATC ^GATC
702 GATC
StuI 191 AGGCCT AGG^CCT
791 AGGCCT
TaqI 37 TCGA T^CGA
375 TCGA
TfiI 27 GAATC G^A[AT]TC
482 GAATC
Tru9I 508 TTAA T^TAA
740 TTAA
TthHB8I 37 TCGA T^CGA
375 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI ApoI AvaI BbvI BmyI
BsaJI BseRI BsmFI BsoBI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboII
MnlI MspI MvaI MwoI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
TseI Tsp45I Tsp509I TspRI