Restriction map for
pFN21AE2038
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI AocI Aor51HI ApaI
ApoI AscI AseI AsnI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaHI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 605 GGTACC G^GTACC
AhdI 1126 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 147 GTGCAC G^TGCAC
ApaLI 147 GTGCAC G^TGCAC
Asp700I 1554 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 605 GGTACC G^GTACC
AspEI 1126 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 368 TGGCCA TGG^CCA
BamHI 513 GGATCC G^GATCC
BsaAI 1456 TACGTG [CT]AC^GT[GA]
BsaBI 1233 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1658 TCCGGT [AT]^CCGG[AT]
BsiEI 674 CGGCCG CG[GA][CT]^CG
BspHI 1505 TCATGA T^CATGA
BspLU11I 373 ACATGT A^CATGT
BsrBI 753 GAGCGG CCGCTC(-3/-3)
BssSI 502 CTCGTG CACGAG(-5/-1)
BstAPI 734 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 869 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 513 GGATCC [GA]^GATC[CT]
DraII 1803 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 1288 GACATGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 674 CGGCCG C^GGCCG
Eam1105I 1126 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 674 CGGCCG C^GGCCG
Eco52I 674 CGGCCG C^GGCCG
EcoO109I 1803 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 973 GATATC GAT^ATC
EcoT14I 1416 CCAAGG C^C[AT][AT]GG
HincII 1176 GTTGAC GT[CT]^[GA]AC
HindII 1176 GTTGAC GT[CT]^[GA]AC
KpnI 605 GGTACC GGTAC^C
KspI 413 CCGCGG CCGC^GG
MamI 1233 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 513 GGATCC [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
NaeI 802 GCCGGC GCC^GGC
NgoMIV 802 GCCGGC G^CCGGC
PpuMI 1803 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 62 CTGCAG CTGCA^G
RcaI 1505 TCATGA T^CATGA
SacI 218 GAGCTC GAGCT^C
SacII 413 CCGCGG CCGC^GG
SapI 1383 GCTCTTC GCTCTTC(1/4)
SgrAI 168 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1839 CTCAAG C^T[CT][GA]AG
SphI 1270 GCATGC GCATG^C
StyI 1416 CCAAGG C^C[AT][AT]GG
TfiI 1757 GATTC G^A[AT]TC
XcmI 10 CCATCTCTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 513 GGATCC [GA]^GATC[CT]
XmnI 1554 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 817 GTCTAC GT^[AC][GT]AC
1684 GTCTAC
AflIII 373 ACATGT A^C[GA][CT]GT
886 ACGTGT
AlwI 514 GATCC GGATC(4/5)
513 GGATC
AlwNI 65 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
240 CAGCAGCTG
BanI 605 GGTACC G^G[CT][GA]CC
1462 GGCACC
BbsI 1305 GAAGAC GAAGAC(2/6)
1069 GTCTTC
BfaI 1589 CTAG C^TAG
1681 CTAG
BglI 281 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
410 GCCCCGCGGGC
BlpI 337 GCTGAGC GC^T[ACGT]AGC
348 GCTTAGC
Bpu1102I 337 GCTGAGC GC^T[ACGT]AGC
348 GCTTAGC
BpuAI 1305 GAAGAC GAAGAC(2/6)
1069 GTCTTC
BsaI 643 GAGACC GGTCTC(1/5)
307 GGTCTC
BseRI 1422 CTCCTC GAGGAG(10/8)
892 GAGGAG
BsrDI 110 GCAATG GCAATG(2/0)
1535 GCAATG
BssHII 631 GCGCGC G^CGCGC
743 GCGCGC
CelII 337 GCTGAGC GC^T[ACGT]AGC
348 GCTTAGC
EarI 1384 CTCTTC CTCTTC(1/4)
1047 GAAGAG
HaeII 831 AGCGCC [GA]GCGC^[CT]
1380 GGCGCT
Ksp632I 1384 CTCTTC CTCTTC(1/4)
1047 GAAGAG
MaeI 1589 CTAG C^TAG
1681 CTAG
PflMI 360 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1605 CCAAGTGGTGG
PvuII 243 CAGCTG CAG^CTG
1042 CAGCTG
ScaI 545 AGTACT AGT^ACT
1556 AGTACT
SmaI 453 CCCGGG CCC^GGG
1151 CCCGGG
StuI 737 AGGCCT AGG^CCT
794 AGGCCT
TaqI 922 TCGA T^CGA
1061 TCGA
TthHB8I 922 TCGA T^CGA
1061 TCGA
Van91I 360 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1605 CCAAGTGGTGG
XmaCI 453 CCCGGG C^CCGGG
1151 CCCGGG
XmaI 453 CCCGGG C^CCGGG
1151 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AspI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI Tth111I