Restriction map for
pFN21AE5413
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
HgaI Hin1I HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 617 CGCG CG^CG
AccIII 594 TCCGGA T^CCGGA
AcsI 436 GAATTT [GA]^AATT[CT]
AflIII 708 ACATGT A^C[GA][CT]GT
AocI 237 CCTGAGG CC^T[ACGT]AGG
ApoI 436 GAATTT [GA]^AATT[CT]
Asp700I 667 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 52 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 62 GAGCCC G[GA]GC[CT]^C
BbvCI 298 GCTGAGG CCTCAGC(-5/-2)
BclI 311 TGATCA T^GATCA
BfaI 748 CTAG C^TAG
BlpI 775 GCTGAGC GC^T[ACGT]AGC
BmyI 62 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 775 GCTGAGC GC^T[ACGT]AGC
BsaWI 594 TCCGGA [AT]^CCGG[AT]
BseAI 594 TCCGGA T^CCGGA
BseRI 598 GAGGAG GAGGAG(10/8)
BsmAI 661 GAGAC GTCTC(1/5)
Bsp1286I 62 GAGCCC G[AGT]GC[ACT]^C
BspEI 594 TCCGGA T^CCGGA
BspHI 314 TCATGA T^CATGA
BspLU11I 708 ACATGT A^CATGT
BspMI 262 ACCTGC ACCTGC(4/8)
BsrDI 537 CATTGC GCAATG(2/0)
BsrI 523 CCAGT ACTGG(1/-1)
BstUI 617 CGCG CG^CG
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
Cac8I 159 GCTTGC GC[ACGT]^[ACGT]GC
CelII 775 GCTGAGC GC^T[ACGT]AGC
CfoI 616 GCGC GCG^C
DraI 822 TTTAAA TTT^AAA
EarI 915 CTCTTC CTCTTC(1/4)
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
FauI 69 CCCGC CCCGC(4/6)
FbaI 311 TGATCA T^GATCA
HhaI 616 GCGC GCG^C
HinP1I 616 GCGC G^CGC
HincII 544 GTCAAC GT[CT]^[GA]AC
HindII 544 GTCAAC GT[CT]^[GA]AC
HinfI 753 GAATC G^A[ACGT]TC
Ksp632I 915 CTCTTC CTCTTC(1/4)
MaeI 748 CTAG C^TAG
MroI 594 TCCGGA T^CCGGA
MslI 727 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 4 CCGCTG C[AC]G^C[GT]G
MvnI 617 CGCG CG^CG
NspI 708 ACATGT [GA]CATG^[CT]
PflFI 52 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 175 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 557 CTGCAG CTGCA^G
RcaI 314 TCATGA T^CATGA
SfcI 557 CTGCAG C^T[GA][CT]AG
TaqI 14 TCGA T^CGA
TfiI 753 GAATC G^A[AT]TC
Tth111I 52 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 14 TCGA T^CGA
Van91I 175 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 667 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 367 GTAC GT^AC
687 GTAC
BcnI 47 CCGGG CC^[GC]GG
498 CCGGG
BglII 805 AGATCT A^GATCT
911 AGATCT
BpmI 839 CTCCAG CTGGAG(16/14)
920 CTCCAG
BsiYI 69 CCCGCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
175 CCAAAGGATGG
BslI 69 CCCGCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
175 CCAAAGGATGG
BstF5I 575 CATCC GGATG(2/0)
180 GGATG
BstYI 805 AGATCT [GA]^GATC[CT]
911 AGATCT
FokI 575 CATCC GGATG(9/13)
180 GGATG
HaeIII 133 GGCC GG^CC
258 GGCC
MflI 805 AGATCT [GA]^GATC[CT]
911 AGATCT
NciI 47 CCGGG CC^[GC]GG
498 CCGGG
NlaIII 315 CATG CATG^
709 CATG
RsaI 367 GTAC GT^AC
687 GTAC
Tsp509I 437 AATT ^AATT
488 AATT
XhoII 805 AGATCT [GA]^GATC[CT]
911 AGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsmFI BssKI BstNI Cfr13I DdeI
DpnII DraII DraIII EcoO109I EcoRII
Fnu4HI HapII HpaII ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIV PpuMI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I TspRI XcmI