Restriction map for
pFN21AE5632
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaI HpaII KasI
KspI MamI MluI MluNI MroI
MscI MslI MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Acc65I 1104 GGTACC G^GTACC
AccI 1464 GTCGAC GT^[AC][GT]AC
Asp700I 1412 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1104 GGTACC G^GTACC
AspHI 670 GAGCAC G[AT]GC[AT]^C
BglII 829 AGATCT A^GATCT
BmyI 670 GAGCAC G[AGT]GC[ACT]^C
BsaAI 641 TACGTA [CT]AC^GT[GA]
BsgI 776 CTGCAC GTGCAG(16/14)
BsiHKAI 670 GAGCAC G[AT]GC[AT]^C
BsmI 659 GAATGC GAATGC(1/-1)
Bsp1286I 670 GAGCAC G[AGT]GC[ACT]^C
BsrDI 136 CATTGC GCAATG(2/0)
BssSI 1205 CTCGTG CACGAG(-5/-1)
BstXI 724 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 829 AGATCT [GA]^GATC[CT]
DraI 351 TTTAAA TTT^AAA
DraIII 1202 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 280 GATATC GAT^ATC
HincII 1464 GTCGAC GT[CT]^[GA]AC
HindII 1464 GTCGAC GT[CT]^[GA]AC
KpnI 1104 GGTACC GGTAC^C
MfeI 807 CAATTG C^AATTG
MflI 829 AGATCT [GA]^GATC[CT]
MunI 807 CAATTG C^AATTG
NspI 1155 ACATGC [GA]CATG^[CT]
SalI 1464 GTCGAC G^TCGAC
SnaBI 641 TACGTA TAC^GTA
XhoII 829 AGATCT [GA]^GATC[CT]
XmnI 1412 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 1105 GTAC GT^AC
1456 GTAC
BanI 963 GGCACC G^G[CT][GA]CC
1104 GGTACC
BfaI 926 CTAG C^TAG
1397 CTAG
BsaI 1240 GAGACC GGTCTC(1/5)
1264 GAGACC
BssKI 146 CCAGG ^CC[ACGT]GG
1270 CCAGG
BstF5I 300 GGATG GGATG(2/0)
813 GGATG
BstNI 146 CCAGG CC^[AT]GG
1270 CCAGG
Cac8I 921 GCAGGC GC[ACGT]^[ACGT]GC
1230 GCCAGC
Cfr13I 69 GGCCC G^G[ACGT]CC
1439 GGCCC
EcoRII 146 CCAGG ^CC[AT]GG
1270 CCAGG
FokI 300 GGATG GGATG(9/13)
813 GGATG
HgaI 121 GACGC GACGC(5/10)
132 GACGC
HindIII 346 AAGCTT A^AGCTT
1415 AAGCTT
MaeI 926 CTAG C^TAG
1397 CTAG
MaeIII 650 GTCAC ^GT[ACGT]AC
697 GTCAC
MvaI 146 CCAGG CC^[AT]GG
1270 CCAGG
PflMI 1021 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1430 CCAAAAAATGG
PstI 32 CTGCAG CTGCA^G
1253 CTGCAG
PvuII 88 CAGCTG CAG^CTG
1099 CAGCTG
RsaI 1105 GTAC GT^AC
1456 GTAC
Sau96I 69 GGCCC G^G[ACGT]CC
1439 GGCCC
ScrFI 146 CCAGG CC^[ACGT]GG
1270 CCAGG
Tsp45I 650 GTCAC ^GT[GC]AC
697 GTCAC
Van91I 1021 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1430 CCAAAAAATGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
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AciI AcsI AluI AlwNI ApoI
BbsI BbvI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsrI DdeI
DpnII EarI Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MseI MspA1I MwoI
NdeII NlaIII NlaIV PleI Sau3AI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I