Restriction map for
pFN21AE1632
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NciI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 184 GTCTAC GT^[AC][GT]AC
AflIII 426 ACATGT A^C[GA][CT]GT
Aor51HI 83 AGCGCT AGC^GCT
ApaI 177 GGGCCC GGGCC^C
AspHI 333 GTGCTC G[AT]GC[AT]^C
BanII 177 GGGCCC G[GA]GC[CT]^C
BsaI 412 GAGACC GGTCTC(1/5)
BsgI 268 CTGCAC GTGCAG(16/14)
BsiHKAI 333 GTGCTC G[AT]GC[AT]^C
BsmAI 412 GAGAC GTCTC(1/5)
BspLU11I 426 ACATGT A^CATGT
BsrFI 212 GCCGGT [GA]^CCGG[CT]
BsrI 301 CCAGT ACTGG(1/-1)
BstEII 318 GGTCACC G^GT[ACGT]ACC
BstPI 318 GGTCACC G^GT[ACGT]ACC
CfoI 84 GCGC GCG^C
Cfr10I 212 GCCGGT [GA]^CCGG[CT]
DdeI 8 CTGAG C^T[ACGT]AG
Eco47III 83 AGCGCT AGC^GCT
EcoO65I 318 GGTCACC G^GT[ACGT]ACC
HaeII 83 AGCGCT [GA]GCGC^[CT]
HhaI 84 GCGC GCG^C
HinP1I 84 GCGC G^CGC
HindIII 44 AAGCTT A^AGCTT
HinfI 194 GAGTC G^A[ACGT]TC
MaeII 110 ACGT A^CGT
MaeIII 319 GTCAC ^GT[ACGT]AC
MboII 263 TCTTC GAAGA(8/7)
MspA1I 242 CAGCTG C[AC]G^C[GT]G
MwoI 77 GCCGTGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 225 CCATGG C^CATGG
NspI 426 ACATGT [GA]CATG^[CT]
PflMI 241 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 194 GAGTC GAGTC(4/5)
PpuMI 145 AGGACCT [GA]G^G[AT]CC[CT]
PstI 361 CTGCAG CTGCA^G
PvuII 242 CAGCTG CAG^CTG
SexAI 415 ACCTGGT A^CC[AT]GGT
SfcI 361 CTGCAG C^T[GA][CT]AG
Tsp45I 319 GTCAC ^GT[GC]AC
Van91I 241 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspI 313 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACCTGGTC
BanI 393 GGCACC G^G[CT][GA]CC
400 GGCACC
BbvI 57 GCTGC GCAGC(8/12)
360 GCTGC
BmyI 177 GGGCCC G[AGT]GC[ACT]^C
333 GTGCTC
BsmFI 115 GTCCC GGGAC(10/14)
298 GTCCC
Bsp1286I 177 GGGCCC G[AGT]GC[ACT]^C
333 GTGCTC
BstF5I 92 CATCC GGATG(2/0)
339 CATCC
Cac8I 165 GCGGGC GC[ACGT]^[ACGT]GC
363 GCAGGC
DsaI 225 CCATGG C^C[GA][CT]GG
368 CCGTGG
EcoT14I 218 CCAAGG C^C[AT][AT]GG
225 CCATGG
FauI 102 GCGGG CCCGC(4/6)
165 GCGGG
FokI 92 CATCC GGATG(9/13)
339 CATCC
HapII 138 CCGG C^CGG
213 CCGG
HpaII 138 CCGG C^CGG
213 CCGG
MspI 138 CCGG C^CGG
213 CCGG
PflFI 313 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACCTGGTC
StyI 218 CCAAGG C^C[AT][AT]GG
225 CCATGG
TseI 57 GCTGC G^C[AT]GC
360 GCTGC
TspRI 125 CACTG CA[GC]TG[ACGT][ACGT]^
302 CAGTG
Tth111I 313 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
414 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsiYI
BslI BssKI BstNI Cfr13I DraII
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI MnlI MvaI NlaIII NlaIV
Sau96I ScrFI