Restriction map for
pFN21AE5941
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstYI
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 831 GTATAC GT^[AC][GT]AC
AciI 5 GCGG CCGC(-3/-1)
AocI 873 CCTGAGG CC^T[ACGT]AGG
Asp700I 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 259 CTCGAG C^[CT]CG[GA]G
AvaII 681 GGTCC G^G[AT]CC
BanI 607 GGTGCC G^G[CT][GA]CC
BanII 896 GGGCTC G[GA]GC[CT]^C
BclI 620 TGATCA T^GATCA
BfaI 343 CTAG C^TAG
BpmI 51 CTCCAG CTGGAG(16/14)
BsoBI 259 CTCGAG C^[CT]CG[GA]G
BspHI 617 TCATGA T^CATGA
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 765 CATTGC GCAATG(2/0)
BsrI 15 CCAGT ACTGG(1/-1)
Bst1107I 831 GTATAC GTA^TAC
BstZ17I 831 GTATAC GTA^TAC
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cfr13I 681 GGTCC G^G[ACGT]CC
DpnII 621 GATC ^GATC
DraII 680 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 748 CTCTTC CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO109I 680 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 5 GCGGG CCCGC(4/6)
FbaI 620 TGATCA T^GATCA
Ksp632I 748 CTCTTC CTCTTC(1/4)
MaeI 343 CTAG C^TAG
MboI 621 GATC ^GATC
MseI 779 TTAA T^TAA
MspA1I 800 CAGCTG C[AC]G^C[GT]G
NdeII 621 GATC ^GATC
NspI 98 GCATGT [GA]CATG^[CT]
PaeR7I 259 CTCGAG C^TCGAG
PpuMI 680 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 800 CAGCTG CAG^CTG
RcaI 617 TCATGA T^CATGA
Sau3AI 621 GATC ^GATC
Sau96I 681 GGTCC G^G[ACGT]CC
SmlI 259 CTCGAG C^T[CT][GA]AG
Tru9I 779 TTAA T^TAA
Tsp509I 334 AATT ^AATT
XhoI 259 CTCGAG C^TCGAG
XmnI 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BalI 107 TGGCCA TGG^CCA
245 TGGCCA
BbvCI 93 CCTCAGC CCTCAGC(-5/-2)
186 CCTCAGC
BspMI 125 ACCTGC ACCTGC(4/8)
664 ACCTGC
BstAPI 474 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
508 GCAGACAATGC
BstXI 110 CCACTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
236 CCAAGATGCTGG
Cac8I 161 GCCTGC GC[ACGT]^[ACGT]GC
243 GCTGGC
DrdI 9 GACAAACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
361 GACCACTTTGTC
EaeI 107 TGGCCA [CT]^GGCC[GA]
245 TGGCCA
EcoNI 579 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
597 CCTTAGTGAGG
MaeIII 541 GTGAC ^GT[ACGT]AC
841 GTGAC
MluNI 107 TGGCCA TGG^CCA
245 TGGCCA
MscI 107 TGGCCA TGG^CCA
245 TGGCCA
SfcI 753 CTACAG C^T[GA][CT]AG
867 CTACAG
TaqI 19 TCGA T^CGA
260 TCGA
Tsp45I 541 GTGAC ^GT[GC]AC
841 GTGAC
TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^
669 CACTG
TthHB8I 19 TCGA T^CGA
260 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MboII MnlI
MslI MvaI MwoI NlaIII NlaIV
PleI RsaI ScrFI SfaNI TseI