Restriction map for
pFN21AE5441
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BalI BamHI BbrPI BbsI
BbvCI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI HindIII HpaI KpnI Ksp632I
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 633 GTAC GT^AC
AocI 909 CCTCAGG CC^T[ACGT]AGG
Aor51HI 443 AGCGCT AGC^GCT
BbeI 754 GGCGCC GGCGC^C
BcgI 789 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 101 GGATAC GTATCC(6/5)
BclI 254 TGATCA T^GATCA
BglI 907 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 11 CTGGAG CTGGAG(16/14)
BsmBI 436 CGTCTC CGTCTC(1/5)
BspMI 226 GCAGGT ACCTGC(4/8)
BsrDI 423 CATTGC GCAATG(2/0)
BsrI 635 ACTGG ACTGG(1/-1)
BstYI 527 GGATCT [GA]^GATC[CT]
Bsu36I 909 CCTCAGG CC^T[ACGT]AGG
DraIII 385 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 610 GACTCGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 882 CCGCGG C^C[GA][CT]GG
Eco47III 443 AGCGCT AGC^GCT
Eco81I 909 CCTCAGG CC^T[ACGT]AGG
FbaI 254 TGATCA T^GATCA
HincII 75 GTCGAC GT[CT]^[GA]AC
HindII 75 GTCGAC GT[CT]^[GA]AC
KasI 754 GGCGCC G^GCGCC
KspI 882 CCGCGG CCGC^GG
MflI 527 GGATCT [GA]^GATC[CT]
NarI 754 GGCGCC GG^CGCC
NspI 671 ACATGC [GA]CATG^[CT]
PaeR7I 439 CTCGAG C^TCGAG
RsaI 633 GTAC GT^AC
SacII 882 CCGCGG CCGC^GG
SalI 75 GTCGAC G^TCGAC
SfoI 754 GGCGCC GGC^GCC
SmaI 814 CCCGGG CCC^GGG
SmlI 439 CTCGAG C^T[CT][GA]AG
XhoI 439 CTCGAG C^TCGAG
XhoII 527 GGATCT [GA]^GATC[CT]
XmaCI 814 CCCGGG C^CCGGG
XmaI 814 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 75 GTCGAC GT^[AC][GT]AC
820 GTCTAC
AcyI 72 GGCGTC G[GA]^CG[CT]C
754 GGCGCC
AlwI 188 GGATC GGATC(4/5)
527 GGATC
ApaI 729 GGGCCC GGGCC^C
905 GGGCCC
AvaI 439 CTCGAG C^[CT]CG[GA]G
814 CCCGGG
AviII 223 TGCGCA TGC^GCA
837 TGCGCA
BsaHI 72 GGCGTC G[GA]^CG[CT]C
754 GGCGCC
BsgI 416 GTGCAG GTGCAG(16/14)
874 GTGCAG
BsmAI 41 GTCTC GTCTC(1/5)
437 GTCTC
BsoBI 439 CTCGAG C^[CT]CG[GA]G
814 CCCGGG
BsrFI 682 GCCGGC [GA]^CCGG[CT]
769 GCCGGC
BssHII 888 GCGCGC G^CGCGC
943 GCGCGC
BstAPI 418 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
867 GCAAGGGGTGC
BstXI 201 CCAGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
924 CCAGGGCTCTGG
Cfr10I 682 GCCGGC [GA]^CCGG[CT]
769 GCCGGC
EaeI 95 CGGCCA [CT]^GGCC[GA]
767 TGGCCG
FspI 223 TGCGCA TGC^GCA
837 TGCGCA
HgaI 73 GCGTC GACGC(5/10)
435 GCGTC
Hin1I 72 GGCGTC G[GA]^CG[CT]C
754 GGCGCC
HinfI 610 GACTC G^A[ACGT]TC
721 GACTC
MboII 204 GAAGA GAAGA(8/7)
858 GAAGA
NaeI 682 GCCGGC GCC^GGC
769 GCCGGC
NgoMIV 682 GCCGGC G^CCGGC
769 GCCGGC
NlaIII 672 CATG CATG^
738 CATG
PleI 610 GACTC GAGTC(4/5)
721 GACTC
PstI 583 CTGCAG CTGCA^G
621 CTGCAG
SfcI 583 CTGCAG C^T[GA][CT]AG
621 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrBI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PflMI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I
Van91I