Restriction map for
pFN21AE4026
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
ApaI ApaLI AscI Asp718I AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 351 CGCG CG^CG
AcsI 379 GAATTT [GA]^AATT[CT]
AhdI 140 GACTCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 229 GATCC GGATC(4/5)
AlwNI 178 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 85 AGCGCT AGC^GCT
ApoI 379 GAATTT [GA]^AATT[CT]
AseI 399 ATTAAT AT^TAAT
AsnI 399 ATTAAT AT^TAAT
Asp700I 198 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 140 GACTCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 35 CCCGGG C^[CT]CG[GA]G
BbsI 586 GAAGAC GAAGAC(2/6)
BpuAI 586 GAAGAC GAAGAC(2/6)
BsaI 281 GAGACC GGTCTC(1/5)
BsgI 625 CTGCAC GTGCAG(16/14)
BsiWI 638 CGTACG C^GTACG
BsmFI 191 GGGAC GGGAC(10/14)
BsoBI 35 CCCGGG C^[CT]CG[GA]G
BspMI 450 GCAGGT ACCTGC(4/8)
BsrFI 30 GCCGGC [GA]^CCGG[CT]
BstUI 351 CGCG CG^CG
BstXI 108 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 30 GCCGGC [GA]^CCGG[CT]
DpnII 229 GATC ^GATC
DraII 191 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 647 CGGCCA [CT]^GGCC[GA]
Eam1105I 140 GACTCAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 85 AGCGCT AGC^GCT
EcoNI 348 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 191 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 617 CCAAGG C^C[AT][AT]GG
FauI 52 GCGGG CCCGC(4/6)
MaeII 146 ACGT A^CGT
MboI 229 GATC ^GATC
MboII 586 GAAGA GAAGA(8/7)
MslI 504 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 78 CCGCTG C[AC]G^C[GT]G
MvnI 351 CGCG CG^CG
NaeI 30 GCCGGC GCC^GGC
NdeII 229 GATC ^GATC
NgoMIV 30 GCCGGC G^CCGGC
NspI 465 GCATGT [GA]CATG^[CT]
PpuMI 191 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 399 ATTAAT AT^TAAT
Sau3AI 229 GATC ^GATC
SmaI 35 CCCGGG CCC^GGG
SmlI 693 CTCAAG C^T[CT][GA]AG
StyI 617 CCAAGG C^C[AT][AT]GG
TfiI 9 GAATC G^A[AT]TC
XmaCI 35 CCCGGG C^CCGGG
XmaI 35 CCCGGG C^CCGGG
XmnI 198 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 97 GGCGCC G[GA]^CG[CT]C
262 GGCGCC
AspHI 19 GAGCTC G[AT]GC[AT]^C
499 GAGCTC
AvaII 135 GGTCC G^G[AT]CC
192 GGACC
BbeI 97 GGCGCC GGCGC^C
262 GGCGCC
BsaHI 97 GGCGCC G[GA]^CG[CT]C
262 GGCGCC
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
499 GAGCTC
BstF5I 252 GGATG GGATG(2/0)
698 GGATG
DdeI 243 CTCAG C^T[ACGT]AG
316 CTGAG
FokI 252 GGATG GGATG(9/13)
698 GGATG
Hin1I 97 GGCGCC G[GA]^CG[CT]C
262 GGCGCC
KasI 97 GGCGCC G^GCGCC
262 GGCGCC
NarI 97 GGCGCC GG^CGCC
262 GGCGCC
NlaIII 466 CATG CATG^
522 CATG
PleI 140 GACTC GAGTC(4/5)
241 GACTC
PstI 81 CTGCAG CTGCA^G
481 CTGCAG
SacI 19 GAGCTC GAGCT^C
499 GAGCTC
SfaNI 44 GCATC GCATC(5/9)
557 GCATC
SfcI 81 CTGCAG C^T[GA][CT]AG
481 CTGCAG
SfoI 97 GGCGCC GGC^GCC
262 GGCGCC
TaqI 275 TCGA T^CGA
542 TCGA
Tsp509I 380 AATT ^AATT
423 AATT
TspRI 13 CACTG CA[GC]TG[ACGT][ACGT]^
484 CAGTG
TthHB8I 275 TCGA T^CGA
542 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI BanI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MnlI MseI MspI MvaI MwoI
NciI NlaIV RsaI Sau96I ScrFI
Tru9I TseI