Restriction map for
pFN21AE6356
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsaHI
BseAI BseRI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 833 GGTACC G^GTACC
AccI 760 GTCTAC GT^[AC][GT]AC
AccII 500 CGCG CG^CG
AflIII 182 ACGTGT A^C[GA][CT]GT
AlwNI 824 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 636 GGGCCC GGGCC^C
Asp718I 833 GGTACC G^GTACC
BbrPI 181 CACGTG CAC^GTG
BfaI 889 CTAG C^TAG
BlpI 201 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 201 GCTCAGC GC^T[ACGT]AGC
BsaI 568 GAGACC GGTCTC(1/5)
BsaWI 470 TCCGGT [AT]^CCGG[AT]
BssSI 767 CTCGTG CACGAG(-5/-1)
BstEII 480 GGTGACC G^GT[ACGT]ACC
BstPI 480 GGTGACC G^GT[ACGT]ACC
BstUI 500 CGCG CG^CG
CelII 201 GCTCAGC GC^T[ACGT]AGC
CfoI 140 GCGC GCG^C
EarI 877 CTCTTC CTCTTC(1/4)
EcoO65I 480 GGTGACC G^GT[ACGT]ACC
FseI 79 GGCCGGCC GGCCGG^CC
HhaI 140 GCGC GCG^C
HinP1I 140 GCGC G^CGC
KpnI 833 GGTACC GGTAC^C
Ksp632I 877 CTCTTC CTCTTC(1/4)
MaeI 889 CTAG C^TAG
MslI 143 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 500 CGCG CG^CG
NcoI 148 CCATGG C^CATGG
NotI 737 GCGGCCGC GC^GGCCGC
NspI 620 ACATGC [GA]CATG^[CT]
PflMI 70 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 181 CACGTG CAC^GTG
PmlI 181 CACGTG CAC^GTG
PpuMI 244 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 224 CAGCTG CAG^CTG
SacI 388 GAGCTC GAGCT^C
SexAI 266 ACCAGGT A^CC[AT]GGT
SfiI 3 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 443 AGGCCT AGG^CCT
Van91I 70 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 178 GTGCAC G^TGCAC
271 GTGCAC
AocI 54 CCTCAGG CC^T[ACGT]AGG
347 CCTCAGG
ApaLI 178 GTGCAC G^TGCAC
271 GTGCAC
AvaII 245 GGTCC G^G[AT]CC
362 GGACC
BalI 145 TGGCCA TGG^CCA
908 TGGCCA
BanI 694 GGTGCC G^G[CT][GA]CC
833 GGTACC
BglI 4 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
593 GCCCCGGGGGC
BsaAI 181 CACGTG [CT]AC^GT[GA]
781 TACGTG
BspMI 571 ACCTGC ACCTGC(4/8)
87 GCAGGT
Bsu36I 54 CCTCAGG CC^T[ACGT]AGG
347 CCTCAGG
DsaI 148 CCATGG C^C[GA][CT]GG
554 CCGTGG
Eco81I 54 CCTCAGG CC^T[ACGT]AGG
347 CCTCAGG
EcoNI 435 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
534 CCTGCGGGAGG
EcoT14I 6 CCTTGG C^C[AT][AT]GG
148 CCATGG
MaeII 182 ACGT A^CGT
782 ACGT
MboII 863 TCTTC GAAGA(8/7)
878 TCTTC
MluNI 145 TGGCCA TGG^CCA
908 TGGCCA
MscI 145 TGGCCA TGG^CCA
908 TGGCCA
MspA1I 224 CAGCTG C[AC]G^C[GT]G
366 CAGCGG
NaeI 80 GCCGGC GCC^GGC
136 GCCGGC
NgoMIV 80 GCCGGC G^CCGGC
136 GCCGGC
StyI 6 CCTTGG C^C[AT][AT]GG
148 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI BamHI BanII BbvI BcnI
BmyI BpmI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI TseI Tsp45I TspRI
XhoII XmaCI XmaI