Restriction map for
pFN21AE4109
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApoI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbeI
BbsI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstF5I BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FokI FseI HindIII HinfI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PflFI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwI 300 GATCC GGATC(4/5)
Aor51HI 602 AGCGCT AGC^GCT
ApaI 213 GGGCCC GGGCC^C
AscI 867 GGCGCGCC GG^CGCGCC
Asp700I 462 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 284 TGGCCA TGG^CCA
BbrPI 835 CACGTG CAC^GTG
BcgI 1213 CGATGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1120 CTAG C^TAG
BsiEI 274 CGGCCG CG[GA][CT]^CG
BsmBI 525 CGTCTC CGTCTC(1/5)
BsrBI 271 GAGCGG CCGCTC(-3/-3)
BsrFI 414 GCCGGT [GA]^CCGG[CT]
BsrI 1162 ACTGG ACTGG(1/-1)
BstEII 996 GGTCACC G^GT[ACGT]ACC
BstPI 996 GGTCACC G^GT[ACGT]ACC
BstXI 80 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 299 AGATCC [GA]^GATC[CT]
Cfr10I 414 GCCGGT [GA]^CCGG[CT]
DraII 212 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 928 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 274 CGGCCG C^GGCCG
EclXI 274 CGGCCG C^GGCCG
Eco47III 602 AGCGCT AGC^GCT
Eco52I 274 CGGCCG C^GGCCG
EcoO109I 212 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 996 GGTCACC G^GT[ACGT]ACC
HincII 937 GTCAAC GT[CT]^[GA]AC
HindII 937 GTCAAC GT[CT]^[GA]AC
MaeI 1120 CTAG C^TAG
MflI 299 AGATCC [GA]^GATC[CT]
MluNI 284 TGGCCA TGG^CCA
MscI 284 TGGCCA TGG^CCA
NotI 273 GCGGCCGC GC^GGCCGC
NspI 423 GCATGC [GA]CATG^[CT]
PaeR7I 628 CTCGAG C^TCGAG
PmaCI 835 CACGTG CAC^GTG
PmlI 835 CACGTG CAC^GTG
SgrAI 413 CGCCGGTG C[GA]^CCGG[CT]G
SphI 423 GCATGC GCATG^C
XcmI 923 CCAACCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 628 CTCGAG C^TCGAG
XhoII 299 AGATCC [GA]^GATC[CT]
XmnI 462 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 523 GGCGTC G[GA]^CG[CT]C
772 GACGCC
Alw44I 219 GTGCAC G^TGCAC
946 GTGCAC
ApaLI 219 GTGCAC G^TGCAC
946 GTGCAC
AvaII 235 GGACC G^G[AT]CC
710 GGTCC
AviII 1034 TGCGCA TGC^GCA
1043 TGCGCA
BbvCI 242 CCTCAGC CCTCAGC(-5/-2)
1167 CCTCAGC
BsaAI 835 CACGTG [CT]AC^GT[GA]
1096 TACGTG
BsaHI 523 GGCGTC G[GA]^CG[CT]C
772 GACGCC
BseRI 1123 GAGGAG GAGGAG(10/8)
1348 GAGGAG
BsgI 1111 CTGCAC GTGCAG(16/14)
1330 GTGCAG
BsmAI 526 GTCTC GTCTC(1/5)
1281 GTCTC
BsmFI 192 GTCCC GGGAC(10/14)
1191 GTCCC
DpnII 300 GATC ^GATC
918 GATC
DsaI 287 CCACGG C^C[GA][CT]GG
927 CCACGG
EarI 313 CTCTTC CTCTTC(1/4)
1390 CTCTTC
FauI 1296 CCCGC CCCGC(4/6)
1418 CCCGC
FspI 1034 TGCGCA TGC^GCA
1043 TGCGCA
Hin1I 523 GGCGTC G[GA]^CG[CT]C
772 GACGCC
Ksp632I 313 CTCTTC CTCTTC(1/4)
1390 CTCTTC
MboI 300 GATC ^GATC
918 GATC
NdeII 300 GATC ^GATC
918 GATC
PflMI 392 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCACGGCGTGG
PvuII 793 CAGCTG CAG^CTG
1333 CAGCTG
SapI 312 GCTCTTC GCTCTTC(1/4)
1389 GCTCTTC
Sau3AI 300 GATC ^GATC
918 GATC
SbfI 477 CCTGCAGG CCTGCA^GG
822 CCTGCAGG
SmlI 598 CTCAAG C^T[CT][GA]AG
628 CTCGAG
Sse8387I 477 CCTGCAGG CCTGCA^GG
822 CCTGCAGG
TspRI 188 CACTG CA[GC]TG[ACGT][ACGT]^
1161 CACTG
Van91I 392 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
927 CCACGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BspMI BssHII BssKI
BssSI BstAPI BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI EaeI
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PstI RsaI SacI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TseI Tsp45I TthHB8I XmaCI
XmaI