Restriction map for
pFN21AE5652
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BcnI BfrI BlnI BpmI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspEI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
MfeI MluI MroI MunI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 713 GGCGCC G[GA]^CG[CT]C
AflIII 164 ACGTGT A^C[GA][CT]GT
Alw44I 982 GTGCAC G^TGCAC
AlwNI 95 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 982 GTGCAC G^TGCAC
Asp700I 1140 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 172 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 713 GGCGCC GGCGC^C
BciVI 446 GGATAC GTATCC(6/5)
BclI 866 TGATCA T^GATCA
BlpI 100 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 100 GCTGAGC GC^T[ACGT]AGC
BsaBI 1227 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 713 GGCGCC G[GA]^CG[CT]C
BsgI 335 CTGCAC GTGCAG(16/14)
BsmI 1460 GCATTC GAATGC(1/-1)
Bsp1407I 704 TGTACA T^GTACA
BspDI 559 ATCGAT AT^CGAT
BspHI 1064 TCATGA T^CATGA
BsrGI 704 TGTACA T^GTACA
BssSI 1257 CACGAG CACGAG(-5/-1)
BstEII 816 GGTGACC G^GT[ACGT]ACC
BstPI 816 GGTGACC G^GT[ACGT]ACC
BstXI 887 CCACCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 100 GCTGAGC GC^T[ACGT]AGC
ClaI 559 ATCGAT AT^CGAT
EarI 579 CTCTTC CTCTTC(1/4)
EcoO65I 816 GGTGACC G^GT[ACGT]ACC
EcoT14I 1415 CCATGG C^C[AT][AT]GG
FauI 12 CCCGC CCCGC(4/6)
FbaI 866 TGATCA T^GATCA
Hin1I 713 GGCGCC G[GA]^CG[CT]C
KasI 713 GGCGCC G^GCGCC
Ksp632I 579 CTCTTC CTCTTC(1/4)
KspI 43 CCGCGG CCGC^GG
MamI 1227 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 464 TTAA T^TAA
NarI 713 GGCGCC GG^CGCC
NcoI 1415 CCATGG C^CATGG
PflFI 172 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1159 GAGTC GAGTC(4/5)
PvuII 208 CAGCTG CAG^CTG
RcaI 1064 TCATGA T^CATGA
SacII 43 CCGCGG CCGC^GG
SapI 578 GCTCTTC GCTCTTC(1/4)
SfoI 713 GGCGCC GGC^GCC
SspBI 704 TGTACA T^GTACA
StyI 1415 CCATGG C^C[AT][AT]GG
Tru9I 464 TTAA T^TAA
Tth111I 172 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 200 CCATGTCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1140 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 44 CGCG CG^CG
1153 CGCG
AvaII 539 GGTCC G^G[AT]CC
567 GGTCC
BalI 83 TGGCCA TGG^CCA
1499 TGGCCA
BanI 713 GGCGCC G^G[CT][GA]CC
1120 GGTGCC
BfaI 1268 CTAG C^TAG
1465 CTAG
BglI 39 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
91 GCCTCAGAGGC
BglII 344 AGATCT A^GATCT
677 AGATCT
BsaI 244 GAGACC GGTCTC(1/5)
874 GAGACC
BseRI 838 CTCCTC GAGGAG(10/8)
673 GAGGAG
BspMI 791 ACCTGC ACCTGC(4/8)
1043 ACCTGC
BstUI 44 CGCG CG^CG
1153 CGCG
BstYI 344 AGATCT [GA]^GATC[CT]
677 AGATCT
DraII 88 AGGGCCT [GA]G^G[ACGT]CC[CT]
1108 AGGCCCC
DsaI 43 CCGCGG C^C[GA][CT]GG
1415 CCATGG
EcoO109I 88 AGGGCCT [GA]G^G[ACGT]CC[CT]
1108 AGGCCCC
HaeII 30 GGCGCT [GA]GCGC^[CT]
713 GGCGCC
MaeI 1268 CTAG C^TAG
1465 CTAG
MflI 344 AGATCT [GA]^GATC[CT]
677 AGATCT
MluNI 83 TGGCCA TGG^CCA
1499 TGGCCA
MscI 83 TGGCCA TGG^CCA
1499 TGGCCA
MslI 1059 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1449 CACCCTGGTG
MspA1I 43 CCGCGG C[AC]G^C[GT]G
208 CAGCTG
MvnI 44 CGCG CG^CG
1153 CGCG
PflMI 234 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
248 CCAACCTGTGG
SacI 468 GAGCTC GAGCT^C
1126 GAGCTC
SfaNI 1285 GATGC GCATC(5/9)
1510 GATGC
SfcI 155 CTGTAG C^T[GA][CT]AG
987 CTACAG
TfiI 1276 GAATC G^A[AT]TC
1389 GATTC
Tsp509I 1141 AATT ^AATT
1240 AATT
Van91I 234 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
248 CCAACCTGTGG
XhoII 344 AGATCT [GA]^GATC[CT]
677 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbsI BbvI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SmlI TaqI
TseI Tsp45I TspRI TthHB8I