Restriction map for
pFN21AE3964
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BlnI BmyI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 686 GCGG CCGC(-3/-1)
AclI 709 AACGTT AA^CGTT
AlwI 822 GGATC GGATC(4/5)
Asp700I 851 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 362 TGGCCA TGG^CCA
BbvI 235 GCTGC GCAGC(8/12)
BclI 813 TGATCA T^GATCA
BcnI 597 CCCGG CC^[GC]GG
BglI 664 GCCAATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 644 AGATCT A^GATCT
BlpI 681 GCTGAGC GC^T[ACGT]AGC
BpmI 178 CTGGAG CTGGAG(16/14)
Bpu1102I 681 GCTGAGC GC^T[ACGT]AGC
BsmAI 97 GAGAC GTCTC(1/5)
BsmI 715 GCATTC GAATGC(1/-1)
Bsp1407I 654 TGTACA T^GTACA
BsrBI 684 GAGCGG CCGCTC(-3/-3)
BsrDI 231 CATTGC GCAATG(2/0)
BsrGI 654 TGTACA T^GTACA
BssSI 435 CACGAG CACGAG(-5/-1)
BstYI 644 AGATCT [GA]^GATC[CT]
CelII 681 GCTGAGC GC^T[ACGT]AGC
DraII 312 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 22 CTCTTC CTCTTC(1/4)
EcoNI 126 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 312 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 813 TGATCA T^GATCA
HapII 598 CCGG C^CGG
HpaII 598 CCGG C^CGG
Ksp632I 22 CTCTTC CTCTTC(1/4)
MaeII 710 ACGT A^CGT
MboII 23 TCTTC GAAGA(8/7)
MflI 644 AGATCT [GA]^GATC[CT]
MluNI 362 TGGCCA TGG^CCA
MscI 362 TGGCCA TGG^CCA
MspA1I 190 CAGCTG C[AC]G^C[GT]G
MspI 598 CCGG C^CGG
NciI 597 CCCGG CC^[GC]GG
NspI 660 ACATGC [GA]CATG^[CT]
PflMI 354 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 312 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 99 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 709 AACGTT AA^CGTT
PvuII 190 CAGCTG CAG^CTG
SexAI 125 ACCTGGT A^CC[AT]GGT
SfcI 166 CTACAG C^T[GA][CT]AG
SspBI 654 TGTACA T^GTACA
TseI 235 GCTGC G^C[AT]GC
Van91I 354 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 644 AGATCT [GA]^GATC[CT]
XmnI 851 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 445 AAATTC [GA]^AATT[CT]
890 AAATTC
AfaI 12 GTAC GT^AC
655 GTAC
AlwNI 311 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
355 CAGTATCTG
ApoI 445 AAATTC [GA]^AATT[CT]
890 AAATTC
AvaII 162 GGACC G^G[AT]CC
313 GGACC
BfaI 118 CTAG C^TAG
487 CTAG
Cfr13I 162 GGACC G^G[ACGT]CC
313 GGACC
EaeI 362 TGGCCA [CT]^GGCC[GA]
687 CGGCCA
Fnu4HI 235 GCTGC GC^[ACGT]GC
686 GCGGC
HphI 129 GGTGA GGTGA(8/7)
816 TCACC
ItaI 235 GCTGC GC^[ACGT]GC
686 GCGGC
MaeI 118 CTAG C^TAG
487 CTAG
MaeIII 106 GTCAC ^GT[ACGT]AC
616 GTCAC
MslI 43 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
895 CACATCTATG
NlaIV 112 GGCTCC GG[ACGT]^[ACGT]CC
496 GGAGCC
PleI 601 GACTC GAGTC(4/5)
174 GAGTC
RsaI 12 GTAC GT^AC
655 GTAC
Sau96I 162 GGACC G^G[ACGT]CC
313 GGACC
SspI 422 AATATT AAT^ATT
517 AATATT
TfiI 349 GATTC G^A[AT]TC
509 GAATC
Tsp45I 106 GTCAC ^GT[GC]AC
616 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII FokI HaeIII
HinfI MboI MnlI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfaNI
Tsp509I TspRI