Restriction map for
pFN21AE4140
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI Asp700I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI Eam1105I EarI Eco81I EcoO65I
EcoRI EcoT22I FseI FspI HindIII
HpaI Ksp632I KspI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1238 GGTACC G^GTACC
AccI 1601 GTCGAC GT^[AC][GT]AC
AflIII 1346 ACATGT A^C[GA][CT]GT
Aor51HI 214 AGCGCT AGC^GCT
ApaI 223 GGGCCC GGGCC^C
AseI 490 ATTAAT AT^TAAT
AsnI 490 ATTAAT AT^TAAT
Asp718I 1238 GGTACC G^GTACC
BciVI 347 GTATCC GTATCC(6/5)
BclI 743 TGATCA T^GATCA
BsiEI 314 CGGCCG CG[GA][CT]^CG
BsmBI 193 GAGACG CGTCTC(1/5)
BsmI 1497 GAATGC GAATGC(1/-1)
Bsp1407I 1418 TGTACA T^GTACA
BspLU11I 1346 ACATGT A^CATGT
BsrBI 169 GAGCGG CCGCTC(-3/-3)
BsrGI 1418 TGTACA T^GTACA
BstXI 653 CCAAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 222 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 334 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1132 CCATGG C^C[GA][CT]GG
EaeI 314 CGGCCG [CT]^GGCC[GA]
EagI 314 CGGCCG C^GGCCG
EclXI 314 CGGCCG C^GGCCG
Eco47III 214 AGCGCT AGC^GCT
Eco52I 314 CGGCCG C^GGCCG
EcoNI 1371 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 222 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 709 GATATC GAT^ATC
EcoT14I 1132 CCATGG C^C[AT][AT]GG
FbaI 743 TGATCA T^GATCA
HgaI 158 GCGTC GACGC(5/10)
HphI 1311 GGTGA GGTGA(8/7)
KpnI 1238 GGTACC GGTAC^C
MspA1I 63 CAGCGG C[AC]G^C[GT]G
NcoI 1132 CCATGG C^CATGG
PshBI 490 ATTAAT AT^TAAT
SalI 1601 GTCGAC G^TCGAC
SmaI 148 CCCGGG CCC^GGG
SspBI 1418 TGTACA T^GTACA
SspI 641 AATATT AAT^ATT
StyI 1132 CCATGG C^C[AT][AT]GG
Tsp45I 1045 GTCAC ^GT[GC]AC
XbaI 1491 TCTAGA T^CTAGA
XmaCI 148 CCCGGG C^CCGGG
XmaI 148 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 148 CCCGGG C^[CT]CG[GA]G
1515 CTCGGG
BanII 145 GAGCCC G[GA]GC[CT]^C
223 GGGCCC
BbeI 67 GGCGCC GGCGC^C
899 GGCGCC
BbsI 88 GAAGAC GAAGAC(2/6)
1429 GTCTTC
BfaI 1220 CTAG C^TAG
1492 CTAG
BpmI 460 CTGGAG CTGGAG(16/14)
633 CTGGAG
BpuAI 88 GAAGAC GAAGAC(2/6)
1429 GTCTTC
BsgI 48 GTGCAG GTGCAG(16/14)
1363 GTGCAG
BsmAI 193 GAGAC GTCTC(1/5)
507 GTCTC
BsmFI 173 GGGAC GGGAC(10/14)
1031 GTCCC
BsoBI 148 CCCGGG C^[CT]CG[GA]G
1515 CTCGGG
BstAPI 1198 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1275 GCAGAGCCTGC
Cac8I 266 GCGGGC GC[ACGT]^[ACGT]GC
1280 GCCTGC
HincII 358 GTCAAC GT[CT]^[GA]AC
1601 GTCGAC
HindII 358 GTCAAC GT[CT]^[GA]AC
1601 GTCGAC
KasI 67 GGCGCC G^GCGCC
899 GGCGCC
MaeI 1220 CTAG C^TAG
1492 CTAG
NarI 67 GGCGCC GG^CGCC
899 GGCGCC
PleI 4 GACTC GAGTC(4/5)
1599 GAGTC
PstI 1231 CTGCAG CTGCA^G
1552 CTGCAG
SfcI 1231 CTGCAG C^T[GA][CT]AG
1552 CTGCAG
SfoI 67 GGCGCC GGC^GCC
899 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI BanI BbvI BcnI
BglII BmyI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII