Restriction map for
pFN21AE6703
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Aor51HI
AscI Asp718I AspEI AspI AviII
AvrII BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MroI MunI MvnI
NciI NcoI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 2330 AACGTT AA^CGTT
Alw44I 706 GTGCAC G^TGCAC
AlwNI 1235 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2285 GGGCCC GGGCC^C
ApaLI 706 GTGCAC G^TGCAC
AseI 2244 ATTAAT AT^TAAT
AsnI 2244 ATTAAT AT^TAAT
AspHI 706 GTGCAC G[AT]GC[AT]^C
AvaI 398 CTCGAG C^[CT]CG[GA]G
BalI 2384 TGGCCA TGG^CCA
BanII 2285 GGGCCC G[GA]GC[CT]^C
BbeI 2098 GGCGCC GGCGC^C
BglII 2525 AGATCT A^GATCT
BsaBI 1290 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1972 GTGCAG GTGCAG(16/14)
BsiHKAI 706 GTGCAC G[AT]GC[AT]^C
BsmI 1363 GAATGC GAATGC(1/-1)
BsoBI 398 CTCGAG C^[CT]CG[GA]G
BspHI 1911 TCATGA T^CATGA
BspMI 1822 GCAGGT ACCTGC(4/8)
BsrBI 112 GAGCGG CCGCTC(-3/-3)
BsrDI 10 GCAATG GCAATG(2/0)
BstAPI 2586 GCATCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2341 GGTCACC G^GT[ACGT]ACC
BstPI 2341 GGTCACC G^GT[ACGT]ACC
CfoI 2099 GCGC GCG^C
EcoNI 946 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2341 GGTCACC G^GT[ACGT]ACC
EcoRI 851 GAATTC G^AATTC
HaeII 2098 GGCGCC [GA]GCGC^[CT]
HgaI 1251 GACGC GACGC(5/10)
HhaI 2099 GCGC GCG^C
HinP1I 2099 GCGC G^CGC
KasI 2098 GGCGCC G^GCGCC
MamI 1290 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2384 TGGCCA TGG^CCA
MscI 2384 TGGCCA TGG^CCA
NaeI 1520 GCCGGC GCC^GGC
NarI 2098 GGCGCC GG^CGCC
NdeI 2513 CATATG CA^TATG
NgoMIV 1520 GCCGGC G^CCGGC
NheI 717 GCTAGC G^CTAGC
PaeR7I 398 CTCGAG C^TCGAG
PpuMI 1824 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2244 ATTAAT AT^TAAT
Psp1406I 2330 AACGTT AA^CGTT
RcaI 1911 TCATGA T^CATGA
ScaI 1870 AGTACT AGT^ACT
SfoI 2098 GGCGCC GGC^GCC
SmlI 398 CTCGAG C^T[CT][GA]AG
SpeI 577 ACTAGT A^CTAGT
SspI 67 AATATT AAT^ATT
XhoI 398 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 1251 GACGCC G[GA]^CG[CT]C
2098 GGCGCC
BamHI 600 GGATCC G^GATCC
2068 GGATCC
BciVI 2224 GGATAC GTATCC(6/5)
2320 GGATAC
BmyI 706 GTGCAC G[AGT]GC[ACT]^C
2285 GGGCCC
BsaHI 1251 GACGCC G[GA]^CG[CT]C
2098 GGCGCC
Bsp1286I 706 GTGCAC G[AGT]GC[ACT]^C
2285 GGGCCC
BsrFI 496 GCCGGT [GA]^CCGG[CT]
1520 GCCGGC
Cfr10I 496 GCCGGT [GA]^CCGG[CT]
1520 GCCGGC
DsaI 1656 CCGTGG C^C[GA][CT]GG
2205 CCACGG
EaeI 1518 TGGCCG [CT]^GGCC[GA]
2384 TGGCCA
EcoRV 663 GATATC GAT^ATC
2260 GATATC
EcoT14I 468 CCAAGG C^C[AT][AT]GG
2613 CCTTGG
HapII 497 CCGG C^CGG
1521 CCGG
Hin1I 1251 GACGCC G[GA]^CG[CT]C
2098 GGCGCC
HpaII 497 CCGG C^CGG
1521 CCGG
MspA1I 25 CAGCTG C[AC]G^C[GT]G
746 CAGCTG
MspI 497 CCGG C^CGG
1521 CCGG
PvuII 25 CAGCTG CAG^CTG
746 CAGCTG
SfcI 1156 CTACAG C^T[GA][CT]AG
1918 CTACAG
SnaBI 1609 TACGTA TAC^GTA
1621 TACGTA
StyI 468 CCAAGG C^C[AT][AT]GG
2613 CCTTGG
TaqI 399 TCGA T^CGA
2169 TCGA
TthHB8I 399 TCGA T^CGA
2169 TCGA
XcmI 587 CCAACATACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2290 CCAGTCACTCTGTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AvaII BanI
BbvI BfaI BpmI BsaAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII DraIII EarI
Eco81I EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI