Restriction map for
pFN21AE4003
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MaeI MamI MfeI MluI
MroI MseI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 298 GTCGAC GT^[AC][GT]AC
AlwNI 243 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 207 CCTGAGG CC^T[ACGT]AGG
Aor51HI 254 AGCGCT AGC^GCT
Asp700I 780 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 508 GAGCAC G[AT]GC[AT]^C
AvaII 189 GGACC G^G[AT]CC
BbrPI 511 CACGTG CAC^GTG
BbsI 523 GTCTTC GAAGAC(2/6)
BcgI 483 CGAGGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 791 AGATCT A^GATCT
BpuAI 523 GTCTTC GAAGAC(2/6)
BsaAI 511 CACGTG [CT]AC^GT[GA]
BsaI 486 GGTCTC GGTCTC(1/5)
BsiHKAI 508 GAGCAC G[AT]GC[AT]^C
Bsp1407I 290 TGTACA T^GTACA
BspMI 461 GCAGGT ACCTGC(4/8)
BsrBI 625 CCGCTC CCGCTC(-3/-3)
BsrGI 290 TGTACA T^GTACA
BsrI 266 ACTGG ACTGG(1/-1)
BssHII 47 GCGCGC G^CGCGC
BssSI 529 CACGAG CACGAG(-5/-1)
BstEII 720 GGTCACC G^GT[ACGT]ACC
BstPI 720 GGTCACC G^GT[ACGT]ACC
BstYI 791 AGATCT [GA]^GATC[CT]
Bsu36I 207 CCTGAGG CC^T[ACGT]AGG
DpnII 792 GATC ^GATC
DsaI 84 CCGCGG C^C[GA][CT]GG
Eco47III 254 AGCGCT AGC^GCT
Eco81I 207 CCTGAGG CC^T[ACGT]AGG
EcoO65I 720 GGTCACC G^GT[ACGT]ACC
EcoT14I 434 CCAAGG C^C[AT][AT]GG
HgaI 296 GCGTC GACGC(5/10)
HinfI 771 GACTC G^A[ACGT]TC
KspI 84 CCGCGG CCGC^GG
MaeII 512 ACGT A^CGT
MboI 792 GATC ^GATC
MflI 791 AGATCT [GA]^GATC[CT]
NdeII 792 GATC ^GATC
NspI 519 GCATGT [GA]CATG^[CT]
PaeR7I 55 CTCGAG C^TCGAG
PleI 771 GACTC GAGTC(4/5)
PmaCI 511 CACGTG CAC^GTG
PmlI 511 CACGTG CAC^GTG
PstI 224 CTGCAG CTGCA^G
PvuII 651 CAGCTG CAG^CTG
SacII 84 CCGCGG CCGC^GG
SalI 298 GTCGAC G^TCGAC
SapI 564 GAAGAGC GCTCTTC(1/4)
Sau3AI 792 GATC ^GATC
SmaI 737 CCCGGG CCC^GGG
SmlI 55 CTCGAG C^T[CT][GA]AG
SspBI 290 TGTACA T^GTACA
StyI 434 CCAAGG C^C[AT][AT]GG
XhoI 55 CTCGAG C^TCGAG
XhoII 791 AGATCT [GA]^GATC[CT]
XmaCI 737 CCCGGG C^CCGGG
XmaI 737 CCCGGG C^CCGGG
XmnI 780 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 80 GGCGCC G[GA]^CG[CT]C
759 GGCGCC
AfaI 291 GTAC GT^AC
420 GTAC
AvaI 55 CTCGAG C^[CT]CG[GA]G
737 CCCGGG
BalI 68 TGGCCA TGG^CCA
647 TGGCCA
BbeI 80 GGCGCC GGCGC^C
759 GGCGCC
BpmI 337 CTGGAG CTGGAG(16/14)
559 CTGGAG
BsaHI 80 GGCGCC G[GA]^CG[CT]C
759 GGCGCC
BseRI 178 GAGGAG GAGGAG(10/8)
598 GAGGAG
BsgI 654 CTGCAC GTGCAG(16/14)
514 GTGCAG
BsoBI 55 CTCGAG C^[CT]CG[GA]G
737 CCCGGG
EaeI 68 TGGCCA [CT]^GGCC[GA]
647 TGGCCA
EarI 586 CTCTTC CTCTTC(1/4)
564 GAAGAG
HapII 579 CCGG C^CGG
738 CCGG
Hin1I 80 GGCGCC G[GA]^CG[CT]C
759 GGCGCC
HincII 61 GTCAAC GT[CT]^[GA]AC
298 GTCGAC
HindII 61 GTCAAC GT[CT]^[GA]AC
298 GTCGAC
HpaII 579 CCGG C^CGG
738 CCGG
HphI 431 TCACC GGTGA(8/7)
722 TCACC
KasI 80 GGCGCC G^GCGCC
759 GGCGCC
Ksp632I 586 CTCTTC CTCTTC(1/4)
564 GAAGAG
MaeIII 430 GTCAC ^GT[ACGT]AC
721 GTCAC
MluNI 68 TGGCCA TGG^CCA
647 TGGCCA
MscI 68 TGGCCA TGG^CCA
647 TGGCCA
MspI 579 CCGG C^CGG
738 CCGG
NarI 80 GGCGCC GG^CGCC
759 GGCGCC
RsaI 291 GTAC GT^AC
420 GTAC
SfaNI 693 GATGC GCATC(5/9)
377 GCATC
SfcI 168 CTACAG C^T[GA][CT]AG
224 CTGCAG
SfoI 80 GGCGCC GGC^GCC
759 GGCGCC
Tsp45I 430 GTCAC ^GT[GC]AC
721 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI ApaI BanI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I ItaI MboII MnlI
MspA1I MvaI MvnI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TaqI
TseI TspRI TthHB8I