Restriction map for
pFN21AE2482
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BlnI BsaAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Cfr10I Cfr13I
ClaI CpoI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI NaeI
NarI NciI NdeI NgoMIV NlaIV
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1000 GACGTC GACGT^C
AccI 1257 GTCTAC GT^[AC][GT]AC
AccII 343 CGCG CG^CG
AflII 1263 CTTAAG C^TTAAG
Asp700I 40 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 715 GAGCTC G[AT]GC[AT]^C
AvaI 251 CTCGAG C^[CT]CG[GA]G
BanII 715 GAGCTC G[GA]GC[CT]^C
BclI 885 TGATCA T^GATCA
BfrI 1263 CTTAAG C^TTAAG
BglII 56 AGATCT A^GATCT
BlpI 370 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC
BsaJI 641 CCATGG C^C[ACGT][ACGT]GG
BsaWI 323 ACCGGA [AT]^CCGG[AT]
BsiHKAI 715 GAGCTC G[AT]GC[AT]^C
BsoBI 251 CTCGAG C^[CT]CG[GA]G
BspLU11I 100 ACATGT A^CATGT
BstUI 343 CGCG CG^CG
BstYI 56 AGATCT [GA]^GATC[CT]
CelII 370 GCTGAGC GC^T[ACGT]AGC
CfoI 342 GCGC GCG^C
DraI 1306 TTTAAA TTT^AAA
DrdI 478 GACTCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 641 CCATGG C^C[GA][CT]GG
EcoRV 538 GATATC GAT^ATC
EcoT14I 641 CCATGG C^C[AT][AT]GG
FauI 780 GCGGG CCCGC(4/6)
FbaI 885 TGATCA T^GATCA
HapII 324 CCGG C^CGG
HgaI 381 GACGC GACGC(5/10)
HhaI 342 GCGC GCG^C
HinP1I 342 GCGC G^CGC
HpaII 324 CCGG C^CGG
MfeI 796 CAATTG C^AATTG
MflI 56 AGATCT [GA]^GATC[CT]
MslI 85 CATTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 324 CCGG C^CGG
MunI 796 CAATTG C^AATTG
MvnI 343 CGCG CG^CG
NcoI 641 CCATGG C^CATGG
NheI 1185 GCTAGC G^CTAGC
NspI 100 ACATGT [GA]CATG^[CT]
PaeR7I 251 CTCGAG C^TCGAG
SacI 715 GAGCTC GAGCT^C
SfcI 1204 CTACAG C^T[GA][CT]AG
StyI 641 CCATGG C^C[AT][AT]GG
Tsp45I 569 GTGAC ^GT[GC]AC
TspRI 214 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 915 TCTAGA T^CTAGA
XcmI 351 CCAGCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 251 CTCGAG C^TCGAG
XhoII 56 AGATCT [GA]^GATC[CT]
XmnI 40 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 381 GACGCC G[GA]^CG[CT]C
1000 GACGTC
AflIII 100 ACATGT A^C[GA][CT]GT
1197 ACACGT
AocI 460 CCTGAGG CC^T[ACGT]AGG
1013 CCTTAGG
BbsI 1024 GAAGAC GAAGAC(2/6)
1003 GTCTTC
BfaI 916 CTAG C^TAG
1186 CTAG
BmyI 451 GGGCAC G[AGT]GC[ACT]^C
715 GAGCTC
BpmI 718 CTCCAG CTGGAG(16/14)
362 CTGGAG
BpuAI 1024 GAAGAC GAAGAC(2/6)
1003 GTCTTC
BsaHI 381 GACGCC G[GA]^CG[CT]C
1000 GACGTC
BsiYI 458 CCCCTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
580 CCAGCATCAGG
BslI 458 CCCCTGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
580 CCAGCATCAGG
Bsp1286I 451 GGGCAC G[AGT]GC[ACT]^C
715 GAGCTC
BspMI 694 ACCTGC ACCTGC(4/8)
907 GCAGGT
BsrDI 143 GCAATG GCAATG(2/0)
1387 GCAATG
BsrI 194 CCAGT ACTGG(1/-1)
525 CCAGT
BssKI 361 CCTGG ^CC[ACGT]GG
501 CCTGG
BstF5I 245 GGATG GGATG(2/0)
326 GGATG
BstNI 361 CCTGG CC^[AT]GG
501 CCTGG
Bsu36I 460 CCTGAGG CC^T[ACGT]AGG
1013 CCTTAGG
EaeI 270 TGGCCG [CT]^GGCC[GA]
401 CGGCCA
EarI 1190 CTCTTC CTCTTC(1/4)
712 GAAGAG
Eco81I 460 CCTGAGG CC^T[ACGT]AGG
1013 CCTTAGG
EcoRII 361 CCTGG ^CC[AT]GG
501 CCTGG
FokI 245 GGATG GGATG(9/13)
326 GGATG
HaeIII 271 GGCC GG^CC
402 GGCC
Hin1I 381 GACGCC G[GA]^CG[CT]C
1000 GACGTC
Ksp632I 1190 CTCTTC CTCTTC(1/4)
712 GAAGAG
MaeI 916 CTAG C^TAG
1186 CTAG
MaeIII 437 GTAAC ^GT[ACGT]AC
569 GTGAC
MvaI 361 CCTGG CC^[AT]GG
501 CCTGG
PleI 478 GACTC GAGTC(4/5)
952 GACTC
SapI 712 GAAGAGC GCTCTTC(1/4)
1189 GCTCTTC
ScrFI 361 CCTGG CC^[ACGT]GG
501 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsmAI Cac8I DdeI DpnII
Fnu4HI HinfI HphI ItaI MaeII
MboI MboII MnlI MseI MspA1I
MwoI NdeII NlaIII RsaI Sau3AI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp509I TthHB8I