##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1286I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeII       MaeIII      MamI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
Sse8387I    SspI        StuI        StyI        SwaI        
TaqI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwI          621  GGATC                GGATC(4/5)
AlwNI         330  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
BbsI           63  GAAGAC               GAAGAC(2/6)
BbvI          490  GCTGC                GCAGC(8/12)
BfaI          485  CTAG                 C^TAG
BpmI          409  CTGGAG               CTGGAG(16/14)
BpuAI          63  GAAGAC               GAAGAC(2/6)
BseRI         433  GAGGAG               GAGGAG(10/8)
BsmAI          47  GAGAC                GTCTC(1/5)
Bsp1407I      155  TGTACA               T^GTACA
BspMI         206  ACCTGC               ACCTGC(4/8)
BsrGI         155  TGTACA               T^GTACA
BssKI         575  CCTGG                ^CC[ACGT]GG
BstF5I        174  GGATG                GGATG(2/0)
BstNI         575  CCTGG                CC^[AT]GG
EcoRII        575  CCTGG                ^CC[AT]GG
Fnu4HI        490  GCTGC                GC^[ACGT]GC
FokI          174  GGATG                GGATG(9/13)
HapII         172  CCGG                 C^CGG
HpaII         172  CCGG                 C^CGG
ItaI          490  GCTGC                GC^[ACGT]GC
MaeI          485  CTAG                 C^TAG
MfeI          518  CAATTG               C^AATTG
MslI          117  CACAACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          172  CCGG                 C^CGG
MunI          518  CAATTG               C^AATTG
MvaI          575  CCTGG                CC^[AT]GG
MwoI          348  GCTACAGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         178  GGAGCC               GG[ACGT]^[ACGT]CC
PleI           49  GACTC                GAGTC(4/5)
PstI          208  CTGCAG               CTGCA^G
ScrFI         575  CCTGG                CC^[ACGT]GG
SpeI          484  ACTAGT               A^CTAGT
SphI          357  GCATGC               GCATG^C
SspBI         155  TGTACA               T^GTACA
TseI          490  GCTGC                G^C[AT]GC
XcmI           81  CCAATATACAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI           97  AAATTT               [GA]^AATT[CT]
              109  AAATTT              
AfaI           73  GTAC                 GT^AC
              156  GTAC                
ApoI           97  AAATTT               [GA]^AATT[CT]
              109  AAATTT              
AvaII         414  GGACC                G^G[AT]CC
              534  GGACC               
BsmFI         218  GGGAC                GGGAC(10/14)
              499  GGGAC               
Cac8I         344  GCCAGC               GC[ACGT]^[ACGT]GC
              357  GCATGC              
EcoT22I       355  ATGCAT               ATGCA^T
              359  ATGCAT              
HaeIII        170  GGCC                 GG^CC
              193  GGCC                
NsiI          355  ATGCAT               ATGCA^T
              359  ATGCAT              
NspI          357  GCATGC               [GA]CATG^[CT]
              652  GCATGT              
RsaI           73  GTAC                 GT^AC
              156  GTAC                
SfcI          208  CTGCAG               C^T[GA][CT]AG
              349  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        BsrI        Cfr13I      DpnII       HinfI       
MboI        MboII       MnlI        MseI        NdeII       
NlaIII      Sau3AI      Sau96I      SfaNI       TfiI        
Tru9I       Tsp509I     TspRI