Restriction map for
pFN21AE3141
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 196 CGCG CG^CG
AlwNI 916 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 642 CCTGAGG CC^T[ACGT]AGG
AspHI 199 GAGCAC G[AT]GC[AT]^C
AvaII 481 GGACC G^G[AT]CC
BbrPI 679 CACGTG CAC^GTG
BciVI 952 GGATAC GTATCC(6/5)
BsaAI 679 CACGTG [CT]AC^GT[GA]
BsaI 667 GAGACC GGTCTC(1/5)
BsiHKAI 199 GAGCAC G[AT]GC[AT]^C
BsmAI 667 GAGAC GTCTC(1/5)
BsrFI 181 ACCGGC [GA]^CCGG[CT]
BstUI 196 CGCG CG^CG
BstXI 545 CCACAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 642 CCTGAGG CC^T[ACGT]AGG
Cfr10I 181 ACCGGC [GA]^CCGG[CT]
DraII 480 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 642 CCTGAGG CC^T[ACGT]AGG
EcoO109I 480 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 930 GAATTC G^AATTC
EcoT14I 411 CCTTGG C^C[AT][AT]GG
HapII 182 CCGG C^CGG
HincII 604 GTCAAC GT[CT]^[GA]AC
HindII 604 GTCAAC GT[CT]^[GA]AC
HindIII 362 AAGCTT A^AGCTT
HpaII 182 CCGG C^CGG
HphI 505 GGTGA GGTGA(8/7)
MaeII 680 ACGT A^CGT
MaeIII 337 GTGAC ^GT[ACGT]AC
MseI 620 TTAA T^TAA
MspI 182 CCGG C^CGG
MvnI 196 CGCG CG^CG
NspI 743 ACATGC [GA]CATG^[CT]
PflMI 915 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 287 GAGTC GAGTC(4/5)
PmaCI 679 CACGTG CAC^GTG
PmlI 679 CACGTG CAC^GTG
PpuMI 480 AGGACCT [GA]G^G[AT]CC[CT]
PstI 565 CTGCAG CTGCA^G
SexAI 1016 ACCTGGT A^CC[AT]GGT
SfaNI 918 GATGC GCATC(5/9)
SmlI 943 CTCAAG C^T[CT][GA]AG
StyI 411 CCTTGG C^C[AT][AT]GG
TfiI 784 GAATC G^A[AT]TC
Tru9I 620 TTAA T^TAA
Tsp45I 337 GTGAC ^GT[GC]AC
Van91I 915 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 105 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 195 CCGC CCGC(-3/-1)
701 CCGC
AcsI 649 GAATTT [GA]^AATT[CT]
930 GAATTC
AfaI 213 GTAC GT^AC
418 GTAC
ApoI 649 GAATTT [GA]^AATT[CT]
930 GAATTC
Asp700I 97 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
684 GAAGAAGTTC
BalI 368 TGGCCA TGG^CCA
725 TGGCCA
BamHI 809 GGATCC G^GATCC
938 GGATCC
BmyI 199 GAGCAC G[AGT]GC[ACT]^C
259 GTGCCC
BsaJI 411 CCTTGG C^C[ACGT][ACGT]GG
818 CCCTGG
BseRI 205 CTCCTC GAGGAG(10/8)
925 GAGGAG
Bsp1286I 199 GAGCAC G[AGT]GC[ACT]^C
259 GTGCCC
BsrDI 729 CATTGC GCAATG(2/0)
713 GCAATG
EarI 76 GAAGAG CTCTTC(1/4)
1029 GAAGAG
EcoNI 216 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
819 CCTGGTAGAGG
HinfI 287 GAGTC G^A[ACGT]TC
784 GAATC
Ksp632I 76 GAAGAG CTCTTC(1/4)
1029 GAAGAG
MluNI 368 TGGCCA TGG^CCA
725 TGGCCA
MscI 368 TGGCCA TGG^CCA
725 TGGCCA
MslI 546 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
675 CACTCACGTG
RsaI 213 GTAC GT^AC
418 GTAC
TaqI 150 TCGA T^CGA
446 TCGA
TthHB8I 150 TCGA T^CGA
446 TCGA
XmnI 97 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
684 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AlwI BbvI BfaI BpmI
BsiYI BslI BsrI BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI HaeIII ItaI
MaeI MboI MboII MflI MnlI
MspA1I MvaI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfcI
TseI Tsp509I TspRI XhoII