Restriction map for
pFN21AE1680
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
ItaI KasI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TseI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 224 GGTACC G^GTACC
AcsI 346 GAATTC [GA]^AATT[CT]
AocI 228 CCTGAGG CC^T[ACGT]AGG
ApoI 346 GAATTC [GA]^AATT[CT]
Asp700I 421 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 224 GGTACC G^GTACC
AvaII 260 GGACC G^G[AT]CC
BamHI 23 GGATCC G^GATCC
BanI 224 GGTACC G^G[CT][GA]CC
BbsI 200 GAAGAC GAAGAC(2/6)
BciVI 11 GGATAC GTATCC(6/5)
BfaI 70 CTAG C^TAG
BpmI 107 CTCCAG CTGGAG(16/14)
BpuAI 200 GAAGAC GAAGAC(2/6)
BsiYI 517 CCTGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 517 CCTGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 259 GGGAC GGGAC(10/14)
BsmI 394 GAATGC GAATGC(1/-1)
BspDI 235 ATCGAT AT^CGAT
BstYI 23 GGATCC [GA]^GATC[CT]
Bsu36I 228 CCTGAGG CC^T[ACGT]AGG
Cac8I 91 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 260 GGACC G^G[ACGT]CC
ClaI 235 ATCGAT AT^CGAT
DraII 259 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 203 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 205 CTCTTC CTCTTC(1/4)
Eco81I 228 CCTGAGG CC^T[ACGT]AGG
EcoNI 517 CCTGTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 259 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 346 GAATTC G^AATTC
EcoT22I 452 ATGCAT ATGCA^T
KpnI 224 GGTACC GGTAC^C
Ksp632I 205 CTCTTC CTCTTC(1/4)
MaeI 70 CTAG C^TAG
MaeIII 410 GTAAC ^GT[ACGT]AC
MflI 23 GGATCC [GA]^GATC[CT]
MseI 499 TTAA T^TAA
MwoI 30 GCCAACTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 449 CATATG CA^TATG
NsiI 452 ATGCAT ATGCA^T
PpuMI 259 GGGACCC [GA]G^G[AT]CC[CT]
Sau96I 260 GGACC G^G[ACGT]CC
SfaNI 454 GCATC GCATC(5/9)
SfcI 42 CTACAG C^T[GA][CT]AG
TfiI 513 GATTC G^A[AT]TC
Tru9I 499 TTAA T^TAA
XhoII 23 GGATCC [GA]^GATC[CT]
XmnI 421 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AfaI 77 GTAC GT^AC
225 GTAC
AluI 90 AGCT AG^CT
459 AGCT
DdeI 146 CTGAG C^T[ACGT]AG
229 CTGAG
PleI 105 GACTC GAGTC(4/5)
203 GACTC
RsaI 77 GTAC GT^AC
225 GTAC
TaqI 213 TCGA T^CGA
236 TCGA
TspRI 220 CAGTG CA[GC]TG[ACGT][ACGT]^
305 CAGTG
TthHB8I 213 TCGA T^CGA
236 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AlwI BsrDI DpnII HinfI MboI
MboII MnlI NdeII NlaIII NlaIV
Sau3AI Tsp509I