Restriction map for
pFN21AE3511
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AclI 647 AACGTT AA^CGTT
AocI 276 CCTCAGG CC^T[ACGT]AGG
Asp700I 595 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 532 GGATCC G^GATCC
BbsI 1045 GAAGAC GAAGAC(2/6)
BcnI 59 CCCGG CC^[GC]GG
BglI 97 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1045 GAAGAC GAAGAC(2/6)
BsaI 1153 GGTCTC GGTCTC(1/5)
BsgI 887 GTGCAG GTGCAG(16/14)
BsiEI 136 CGGCCG CG[GA][CT]^CG
BsrFI 138 GCCGGT [GA]^CCGG[CT]
Bsu36I 276 CCTCAGG CC^T[ACGT]AGG
Cfr10I 138 GCCGGT [GA]^CCGG[CT]
DsaI 344 CCATGG C^C[GA][CT]GG
EaeI 136 CGGCCG [CT]^GGCC[GA]
EagI 136 CGGCCG C^GGCCG
EarI 202 CTCTTC CTCTTC(1/4)
EclXI 136 CGGCCG C^GGCCG
Eco52I 136 CGGCCG C^GGCCG
Eco81I 276 CCTCAGG CC^T[ACGT]AGG
EcoNI 19 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 102 GCGGG CCCGC(4/6)
Ksp632I 202 CTCTTC CTCTTC(1/4)
MspA1I 121 CAGCGG C[AC]G^C[GT]G
NciI 59 CCCGG CC^[GC]GG
NcoI 344 CCATGG C^CATGG
PleI 176 GACTC GAGTC(4/5)
PpuMI 1037 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 647 AACGTT AA^CGTT
SacI 7 GAGCTC GAGCT^C
SmlI 537 CTCAAG C^T[CT][GA]AG
SphI 951 GCATGC GCATG^C
StuI 726 AGGCCT AGG^CCT
Tsp45I 522 GTCAC ^GT[GC]AC
Tsp509I 1136 AATT ^AATT
XmnI 595 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 7 GAGCTC G[AT]GC[AT]^C
544 GAGCAC
BanII 7 GAGCTC G[GA]GC[CT]^C
309 GGGCTC
BfaI 65 CTAG C^TAG
475 CTAG
BseRI 4 GAGGAG GAGGAG(10/8)
478 GAGGAG
BsiHKAI 7 GAGCTC G[AT]GC[AT]^C
544 GAGCAC
BstYI 495 AGATCC [GA]^GATC[CT]
532 GGATCC
DpnII 496 GATC ^GATC
533 GATC
DraII 183 AGGCCCT [GA]G^G[ACGT]CC[CT]
1037 AGGTCCT
EcoO109I 183 AGGCCCT [GA]G^G[ACGT]CC[CT]
1037 AGGTCCT
HgaI 91 GACGC GACGC(5/10)
1110 GCGTC
HphI 141 GGTGA GGTGA(8/7)
1128 TCACC
MaeI 65 CTAG C^TAG
475 CTAG
MaeII 633 ACGT A^CGT
648 ACGT
MaeIII 522 GTCAC ^GT[ACGT]AC
1094 GTTAC
MboI 496 GATC ^GATC
533 GATC
MflI 495 AGATCC [GA]^GATC[CT]
532 GGATCC
MseI 1004 TTAA T^TAA
1148 TTAA
MslI 901 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
952 CATGCCCGTG
NdeII 496 GATC ^GATC
533 GATC
NspI 900 ACATGC [GA]CATG^[CT]
951 GCATGC
PflMI 181 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCACTGCCTGG
Sau3AI 496 GATC ^GATC
533 GATC
SfcI 659 CTGTAG C^T[GA][CT]AG
668 CTGTAG
Tru9I 1004 TTAA T^TAA
1148 TTAA
Van91I 181 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCACTGCCTGG
XcmI 181 CCAGGCCCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
665 CCACTGTAGAACTGG
XhoII 495 AGATCC [GA]^GATC[CT]
532 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboII MnlI MspI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI StyI TfiI TseI TspRI