Restriction map for
pFN21AE5356
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AflIII AgeI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco52I Eco81I EcoO65I EcoRV
FseI FspI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MluI MluNI
MscI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 425 TCCGGA T^CCGGA
AclI 892 AACGTT AA^CGTT
AhdI 316 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 234 GGATC GGATC(4/5)
AlwNI 16 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 98 AGCGCT AGC^GCT
AspEI 316 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 478 GAGCTC G[AT]GC[AT]^C
BanI 682 GGCACC G^G[CT][GA]CC
BciVI 576 GGATAC GTATCC(6/5)
BclI 396 TGATCA T^GATCA
BcnI 177 CCGGG CC^[GC]GG
BglI 49 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 464 AGATCT A^GATCT
BsaWI 425 TCCGGA [AT]^CCGG[AT]
BseAI 425 TCCGGA T^CCGGA
BseRI 328 GAGGAG GAGGAG(10/8)
BsgI 470 GTGCAG GTGCAG(16/14)
BsiHKAI 478 GAGCTC G[AT]GC[AT]^C
Bsp1407I 41 TGTACA T^GTACA
BspEI 425 TCCGGA T^CCGGA
BsrGI 41 TGTACA T^GTACA
BstAPI 15 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 224 CCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 464 AGATCT [GA]^GATC[CT]
Cac8I 107 GCCTGC GC[ACGT]^[ACGT]GC
DdeI 206 CTGAG C^T[ACGT]AG
DsaI 230 CCGTGG C^C[GA][CT]GG
EaeI 238 CGGCCA [CT]^GGCC[GA]
Eam1105I 316 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 98 AGCGCT AGC^GCT
EcoRI 166 GAATTC G^AATTC
EcoT14I 53 CCAAGG C^C[AT][AT]GG
EcoT22I 854 ATGCAT ATGCA^T
FauI 84 GCGGG CCCGC(4/6)
FbaI 396 TGATCA T^GATCA
HaeII 98 AGCGCT [GA]GCGC^[CT]
HgaI 721 GACGC GACGC(5/10)
HincII 715 GTCAAC GT[CT]^[GA]AC
HindII 715 GTCAAC GT[CT]^[GA]AC
HphI 914 TCACC GGTGA(8/7)
MflI 464 AGATCT [GA]^GATC[CT]
MroI 425 TCCGGA T^CCGGA
NciI 177 CCGGG CC^[GC]GG
NsiI 854 ATGCAT ATGCA^T
PflMI 379 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 892 AACGTT AA^CGTT
SacI 478 GAGCTC GAGCT^C
SbfI 603 CCTGCAGG CCTGCA^GG
SfaNI 702 GATGC GCATC(5/9)
SmlI 481 CTCAAG C^T[CT][GA]AG
Sse8387I 603 CCTGCAGG CCTGCA^GG
SspBI 41 TGTACA T^GTACA
StuI 638 AGGCCT AGG^CCT
StyI 53 CCAAGG C^C[AT][AT]GG
TaqI 794 TCGA T^CGA
TfiI 728 GATTC G^A[AT]TC
TthHB8I 794 TCGA T^CGA
Van91I 379 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 464 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 166 GAATTC [GA]^AATT[CT]
650 AAATTT
ApoI 166 GAATTC [GA]^AATT[CT]
650 AAATTT
BanII 148 GAGCCC G[GA]GC[CT]^C
478 GAGCTC
BbsI 525 GAAGAC GAAGAC(2/6)
181 GTCTTC
BpuAI 525 GAAGAC GAAGAC(2/6)
181 GTCTTC
BsaJI 53 CCAAGG C^C[ACGT][ACGT]GG
230 CCGTGG
BsmFI 93 GTCCC GGGAC(10/14)
369 GTCCC
BspMI 20 ACCTGC ACCTGC(4/8)
602 ACCTGC
BstF5I 171 CATCC GGATG(2/0)
867 GGATG
CfoI 99 GCGC GCG^C
568 GCGC
DraII 488 AGGTCCT [GA]G^G[ACGT]CC[CT]
518 AGGACCT
EcoNI 21 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
201 CCTCTCTGAGG
EcoO109I 488 AGGTCCT [GA]G^G[ACGT]CC[CT]
518 AGGACCT
FokI 171 CATCC GGATG(9/13)
867 GGATG
HhaI 99 GCGC GCG^C
568 GCGC
HinP1I 99 GCGC G^CGC
568 GCGC
MaeII 125 ACGT A^CGT
893 ACGT
MseI 494 TTAA T^TAA
635 TTAA
NlaIV 197 GGAACC GG[ACGT]^[ACGT]CC
682 GGCACC
NspI 80 ACATGC [GA]CATG^[CT]
836 GCATGT
PleI 322 GAGTC GAGTC(4/5)
547 GAGTC
PpuMI 488 AGGTCCT [GA]G^G[AT]CC[CT]
518 AGGACCT
Tru9I 494 TTAA T^TAA
635 TTAA
TspRI 242 CAGTG CA[GC]TG[ACGT][ACGT]^
542 CACTG
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI Asp700I AvaII
BbvI BmyI BpmI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cfr13I
DpnII EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII PstI RsaI Sau3AI
Sau96I ScrFI SfcI TseI Tsp509I
XmnI