Restriction map for
pFN21AE4029
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AvrII BalI BamHI
BbrPI BbvCI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BsrI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HindIII HpaI
HphI Ksp632I MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp45I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 164 GGTACC G^GTACC
AlwNI 514 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 438 CCTGAGG CC^T[ACGT]AGG
Asp718I 164 GGTACC G^GTACC
AspI 445 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 379 CCCGAG C^[CT]CG[GA]G
AvaII 471 GGACC G^G[AT]CC
BbsI 313 GTCTTC GAAGAC(2/6)
BciVI 303 GGATAC GTATCC(6/5)
BglI 482 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 634 CTGGAG CTGGAG(16/14)
BpuAI 313 GTCTTC GAAGAC(2/6)
BsaWI 231 TCCGGT [AT]^CCGG[AT]
BsgI 337 GTGCAG GTGCAG(16/14)
BsmI 648 GAATGC GAATGC(1/-1)
BsoBI 379 CCCGAG C^[CT]CG[GA]G
BspMI 154 ACCTGC ACCTGC(4/8)
BsrBI 37 CCGCTC CCGCTC(-3/-3)
BsrDI 158 GCAATG GCAATG(2/0)
BsrFI 179 ACCGGC [GA]^CCGG[CT]
BstAPI 92 GCATCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 222 GGATG GGATG(2/0)
BstXI 185 CCACTGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 438 CCTGAGG CC^T[ACGT]AGG
Cfr10I 179 ACCGGC [GA]^CCGG[CT]
EaeI 521 TGGCCG [CT]^GGCC[GA]
Eco81I 438 CCTGAGG CC^T[ACGT]AGG
FokI 222 GGATG GGATG(9/13)
HgaI 567 GACGC GACGC(5/10)
HincII 451 GTCAAC GT[CT]^[GA]AC
HindII 451 GTCAAC GT[CT]^[GA]AC
HinfI 595 GACTC G^A[ACGT]TC
KpnI 164 GGTACC GGTAC^C
MaeII 455 ACGT A^CGT
PflFI 445 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 595 GACTC GAGTC(4/5)
PvuII 466 CAGCTG CAG^CTG
SfaNI 92 GCATC GCATC(5/9)
SfiI 481 GGCCGGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 550 CTTGAG C^T[CT][GA]AG
Tsp509I 462 AATT ^AATT
Tth111I 445 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 118 GGCGCC G[GA]^CG[CT]C
405 GGCGCC
AfaI 165 GTAC GT^AC
457 GTAC
AlwI 384 GATCC GGATC(4/5)
531 GATCC
ApaI 44 GGGCCC GGGCC^C
489 GGGCCC
Asp700I 687 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
690 GAAGTTCTTC
AviII 236 TGCGCA TGC^GCA
662 TGCGCA
BbeI 118 GGCGCC GGCGC^C
405 GGCGCC
BcgI 535 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
134 GCAGCCTCATCG
BlpI 15 GCTGAGC GC^T[ACGT]AGC
557 GCTCAGC
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
557 GCTCAGC
BsaHI 118 GGCGCC G[GA]^CG[CT]C
405 GGCGCC
BssHII 74 GCGCGC G^CGCGC
172 GCGCGC
BstYI 383 AGATCC [GA]^GATC[CT]
530 AGATCC
CelII 15 GCTGAGC GC^T[ACGT]AGC
557 GCTCAGC
DpnII 384 GATC ^GATC
531 GATC
FspI 236 TGCGCA TGC^GCA
662 TGCGCA
HaeII 118 GGCGCC [GA]GCGC^[CT]
405 GGCGCC
Hin1I 118 GGCGCC G[GA]^CG[CT]C
405 GGCGCC
KasI 118 GGCGCC G^GCGCC
405 GGCGCC
KspI 7 CCGCGG CCGC^GG
61 CCGCGG
MboI 384 GATC ^GATC
531 GATC
MflI 383 AGATCC [GA]^GATC[CT]
530 AGATCC
NarI 118 GGCGCC GG^CGCC
405 GGCGCC
NdeII 384 GATC ^GATC
531 GATC
RsaI 165 GTAC GT^AC
457 GTAC
SacI 227 GAGCTC GAGCT^C
244 GAGCTC
SacII 7 CCGCGG CCGC^GG
61 CCGCGG
Sau3AI 384 GATC ^GATC
531 GATC
SfoI 118 GGCGCC GGC^GCC
405 GGCGCC
TspRI 186 CACTG CA[GC]TG[ACGT][ACGT]^
609 CAGTG
XhoII 383 AGATCC [GA]^GATC[CT]
530 AGATCC
XmnI 687 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
690 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AspHI BanI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI