Restriction map for
pFN21AE1933
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspI AviII AvrII BalI BamHI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 55 GGTACC G^GTACC
AccI 469 GTCTAC GT^[AC][GT]AC
AfaI 56 GTAC GT^AC
AflIII 857 ACATGT A^C[GA][CT]GT
AhdI 111 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 172 GATCC GGATC(4/5)
AocI 695 CCTCAGG CC^T[ACGT]AGG
Asp700I 626 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 55 GGTACC G^GTACC
AspEI 111 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 865 GAGCTC G[AT]GC[AT]^C
BbeI 833 GGCGCC GGCGC^C
BbrPI 184 CACGTG CAC^GTG
BglI 676 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 997 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 997 GCTGAGC GC^T[ACGT]AGC
BsaAI 184 CACGTG [CT]AC^GT[GA]
BsaI 1125 GGTCTC GGTCTC(1/5)
BsgI 742 CTGCAC GTGCAG(16/14)
BsiHKAI 865 GAGCTC G[AT]GC[AT]^C
BspLU11I 857 ACATGT A^CATGT
BspMI 1109 GCAGGT ACCTGC(4/8)
BsrBI 556 GAGCGG CCGCTC(-3/-3)
BsrDI 1065 CATTGC GCAATG(2/0)
BstYI 171 AGATCC [GA]^GATC[CT]
Bsu36I 695 CCTCAGG CC^T[ACGT]AGG
CelII 997 GCTGAGC GC^T[ACGT]AGC
DpnII 172 GATC ^GATC
DraII 1376 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 123 TGGCCG [CT]^GGCC[GA]
Eam1105I 111 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 695 CCTCAGG CC^T[ACGT]AGG
EcoNI 723 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1376 AGGACCT [GA]G^G[ACGT]CC[CT]
KasI 833 GGCGCC G^GCGCC
KpnI 55 GGTACC GGTAC^C
MboI 172 GATC ^GATC
MflI 171 AGATCC [GA]^GATC[CT]
MslI 745 CACAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 833 GGCGCC GG^CGCC
NcoI 1088 CCATGG C^CATGG
NdeI 896 CATATG CA^TATG
NdeII 172 GATC ^GATC
PflMI 399 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 184 CACGTG CAC^GTG
PmlI 184 CACGTG CAC^GTG
PpuMI 1376 AGGACCT [GA]G^G[AT]CC[CT]
PstI 724 CTGCAG CTGCA^G
RsaI 56 GTAC GT^AC
SacI 865 GAGCTC GAGCT^C
Sau3AI 172 GATC ^GATC
SfoI 833 GGCGCC GGC^GCC
Van91I 399 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4 TCTAGA T^CTAGA
XhoII 171 AGATCC [GA]^GATC[CT]
XmnI 626 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 179 GACGCC G[GA]^CG[CT]C
833 GGCGCC
AvaI 486 CTCGGG C^[CT]CG[GA]G
842 CCCGAG
BanII 14 GAGCCC G[GA]GC[CT]^C
865 GAGCTC
BsaHI 179 GACGCC G[GA]^CG[CT]C
833 GGCGCC
BsoBI 486 CTCGGG C^[CT]CG[GA]G
842 CCCGAG
BsrFI 64 GCCGGC [GA]^CCGG[CT]
815 GCCGGC
BstAPI 922 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1109 GCAGGTGGTGC
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
815 GCCGGC
DsaI 247 CCACGG C^C[GA][CT]GG
1088 CCATGG
EarI 391 GAAGAG CTCTTC(1/4)
649 GAAGAG
EcoT14I 69 CCTTGG C^C[AT][AT]GG
1088 CCATGG
HaeII 306 GGCGCT [GA]GCGC^[CT]
833 GGCGCC
HgaI 179 GACGC GACGC(5/10)
117 GCGTC
Hin1I 179 GACGCC G[GA]^CG[CT]C
833 GGCGCC
Ksp632I 391 GAAGAG CTCTTC(1/4)
649 GAAGAG
MaeII 185 ACGT A^CGT
496 ACGT
MspA1I 781 CAGCTG C[AC]G^C[GT]G
991 CAGCTG
NaeI 64 GCCGGC GCC^GGC
815 GCCGGC
NgoMIV 64 GCCGGC G^CCGGC
815 GCCGGC
NspI 857 ACATGT [GA]CATG^[CT]
1050 GCATGT
PvuII 781 CAGCTG CAG^CTG
991 CAGCTG
StyI 69 CCTTGG C^C[AT][AT]GG
1088 CCATGG
TfiI 74 GATTC G^A[AT]TC
631 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI