Restriction map for
pFN21AE9433
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BclI
BcnI BfaI BfrI BglI BlnI
BsaAI BsiEI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeI MfeI MunI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 859 GTCTAC GT^[AC][GT]AC
AccIII 734 TCCGGA T^CCGGA
AcsI 361 AAATTT [GA]^AATT[CT]
AcyI 58 GGCGCC G[GA]^CG[CT]C
AflIII 50 ACGCGT A^C[GA][CT]GT
AgeI 1019 ACCGGT A^CCGGT
Aor51HI 729 AGCGCT AGC^GCT
ApoI 361 AAATTT [GA]^AATT[CT]
BalI 515 TGGCCA TGG^CCA
BbeI 58 GGCGCC GGCGC^C
BcgI 83 GCAGCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1291 GTATCC GTATCC(6/5)
BglII 1136 AGATCT A^GATCT
BlpI 1187 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1187 GCTCAGC GC^T[ACGT]AGC
BsaHI 58 GGCGCC G[GA]^CG[CT]C
BsaI 1399 GAGACC GGTCTC(1/5)
BseAI 734 TCCGGA T^CCGGA
BseRI 1382 CTCCTC GAGGAG(10/8)
Bsp1407I 491 TGTACA T^GTACA
BspEI 734 TCCGGA T^CCGGA
BsrBI 1492 GAGCGG CCGCTC(-3/-3)
BsrGI 491 TGTACA T^GTACA
BssHII 86 GCGCGC G^CGCGC
BstXI 809 CCATGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1187 GCTCAGC GC^T[ACGT]AGC
DraIII 604 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 886 GACACTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 515 TGGCCA [CT]^GGCC[GA]
Eco47III 729 AGCGCT AGC^GCT
HgaI 52 GCGTC GACGC(5/10)
Hin1I 58 GGCGCC G[GA]^CG[CT]C
HincII 192 GTCAAC GT[CT]^[GA]AC
HindII 192 GTCAAC GT[CT]^[GA]AC
KasI 58 GGCGCC G^GCGCC
MluI 50 ACGCGT A^CGCGT
MluNI 515 TGGCCA TGG^CCA
MroI 734 TCCGGA T^CCGGA
MscI 515 TGGCCA TGG^CCA
NarI 58 GGCGCC GG^CGCC
NcoI 809 CCATGG C^CATGG
PinAI 1019 ACCGGT A^CCGGT
PleI 1285 GACTC GAGTC(4/5)
PpuMI 997 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1351 CTGCAG CTGCA^G
SexAI 605 ACCTGGT A^CC[AT]GGT
SfaNI 466 GATGC GCATC(5/9)
SfcI 1351 CTGCAG C^T[GA][CT]AG
SfoI 58 GGCGCC GGC^GCC
SspBI 491 TGTACA T^GTACA
TaqI 290 TCGA T^CGA
TfiI 1540 GATTC G^A[AT]TC
Tsp509I 362 AATT ^AATT
TthHB8I 290 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 51 CGCG CG^CG
87 CGCG
AlwNI 1102 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1310 CAGCCCCTG
ApaI 500 GGGCCC GGGCC^C
689 GGGCCC
AspHI 871 GTGCTC G[AT]GC[AT]^C
1275 GAGCAC
AvaII 737 GGACC G^G[AT]CC
998 GGACC
BanII 500 GGGCCC G[GA]GC[CT]^C
689 GGGCCC
BbsI 820 GTCTTC GAAGAC(2/6)
895 GTCTTC
BpuAI 820 GTCTTC GAAGAC(2/6)
895 GTCTTC
BsaBI 489 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
675 GATACTGATC
BsaWI 734 TCCGGA [AT]^CCGG[AT]
1019 ACCGGT
BsiHKAI 871 GTGCTC G[AT]GC[AT]^C
1275 GAGCAC
BsmAI 1399 GAGAC GTCTC(1/5)
931 GTCTC
BsrFI 1019 ACCGGT [GA]^CCGG[CT]
1305 ACCGGC
BstUI 51 CGCG CG^CG
87 CGCG
Cfr10I 1019 ACCGGT [GA]^CCGG[CT]
1305 ACCGGC
DraII 689 GGGCCCT [GA]G^G[ACGT]CC[CT]
997 GGGACCC
DsaI 635 CCACGG C^C[GA][CT]GG
809 CCATGG
EcoO109I 689 GGGCCCT [GA]G^G[ACGT]CC[CT]
997 GGGACCC
FauI 70 GCGGG CCCGC(4/6)
777 GCGGG
HaeII 58 GGCGCC [GA]GCGC^[CT]
729 AGCGCT
HinfI 1285 GACTC G^A[ACGT]TC
1540 GATTC
MaeII 565 ACGT A^CGT
614 ACGT
MamI 489 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
675 GATACTGATC
MseI 239 TTAA T^TAA
1142 TTAA
MvnI 51 CGCG CG^CG
87 CGCG
PflMI 504 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCAGCTCCTGG
PvuII 112 CAGCTG CAG^CTG
1190 CAGCTG
SapI 1450 GAAGAGC GCTCTTC(1/4)
342 GCTCTTC
Tru9I 239 TTAA T^TAA
1142 TTAA
Tsp45I 223 GTCAC ^GT[GC]AC
388 GTGAC
Van91I 504 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BanI BbvCI BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI StyI TseI
TspRI XhoII