Restriction map for
pFN21AE5728
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AocI ApoI AseI
AsnI Asp700I Asp718I AvrII BbsI
BbvCI BciVI BfrI BglII BlnI
BpuAI BsiWI BspDI BspHI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI MfeI MluI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacI
SalI SapI SbfI ScaI SfuI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 1328 TCCGGA T^CCGGA
AflIII 225 ACATGT A^C[GA][CT]GT
AhdI 1396 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1988 AGCGCT AGC^GCT
AscI 2696 GGCGCGCC GG^CGCGCC
AspEI 1396 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2450 TGCGCA TGC^GCA
BclI 1089 TGATCA T^GATCA
BseAI 1328 TCCGGA T^CCGGA
BsmI 406 GAATGC GAATGC(1/-1)
Bsp1407I 473 TGTACA T^GTACA
BspEI 1328 TCCGGA T^CCGGA
BspLU11I 225 ACATGT A^CATGT
BsrDI 321 CATTGC GCAATG(2/0)
BsrGI 473 TGTACA T^GTACA
CpoI 1427 CGGACCG CG^G[AT]CCG
DrdI 1758 GACCACGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1396 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 910 GAAGAG CTCTTC(1/4)
Eco47III 1988 AGCGCT AGC^GCT
FbaI 1089 TGATCA T^GATCA
FspI 2450 TGCGCA TGC^GCA
HincII 2458 GTCAAC GT[CT]^[GA]AC
HindII 2458 GTCAAC GT[CT]^[GA]AC
Ksp632I 910 GAAGAG CTCTTC(1/4)
MroI 1328 TCCGGA T^CCGGA
NcoI 1118 CCATGG C^CATGG
PflFI 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2269 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 304 CTGCAG CTGCA^G
PvuII 2146 CAGCTG CAG^CTG
RsrII 1427 CGGACCG CG^G[AT]CCG
SgrAI 1796 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 473 TGTACA T^GTACA
Tsp45I 1318 GTGAC ^GT[GC]AC
Tsp509I 1450 AATT ^AATT
Tth111I 82 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2269 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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BamHI 1325 GGATCC G^GATCC
1331 GGATCC
BbrPI 394 CACGTG CAC^GTG
2317 CACGTG
BcgI 2676 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
543 GCAGGCAGGTCG
BlpI 365 GCTCAGC GC^T[ACGT]AGC
1857 GCTGAGC
Bpu1102I 365 GCTCAGC GC^T[ACGT]AGC
1857 GCTGAGC
BsaBI 435 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATCCGGATC
BsaWI 1328 TCCGGA [AT]^CCGG[AT]
2091 ACCGGA
BsmBI 375 CGTCTC CGTCTC(1/5)
2095 GAGACG
BssSI 579 CACGAG CACGAG(-5/-1)
1948 CACGAG
BstXI 1541 CCAACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2141 CCACGCAGCTGG
CelII 365 GCTCAGC GC^T[ACGT]AGC
1857 GCTGAGC
DraIII 283 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
570 CACTGTGTG
EcoT14I 1037 CCTTGG C^C[AT][AT]GG
1118 CCATGG
HphI 2626 GGTGA GGTGA(8/7)
1925 TCACC
KspI 1819 CCGCGG CCGC^GG
2186 CCGCGG
MaeIII 684 GTTAC ^GT[ACGT]AC
1318 GTGAC
MamI 435 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATCCGGATC
MseI 943 TTAA T^TAA
979 TTAA
NotI 2035 GCGGCCGC GC^GGCCGC
2076 GCGGCCGC
NspI 225 ACATGT [GA]CATG^[CT]
636 ACATGC
PmaCI 394 CACGTG CAC^GTG
2317 CACGTG
PmlI 394 CACGTG CAC^GTG
2317 CACGTG
SacII 1819 CCGCGG CCGC^GG
2186 CCGCGG
SexAI 123 ACCTGGT A^CC[AT]GGT
677 ACCTGGT
SfcI 304 CTGCAG C^T[GA][CT]AG
2424 CTACAG
SfiI 1600 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2635 GGCCGTGGCGGCC
SmlI 2155 CTCAAG C^T[CT][GA]AG
2161 CTCAAG
StyI 1037 CCTTGG C^C[AT][AT]GG
1118 CCATGG
TfiI 1168 GATTC G^A[AT]TC
1435 GATTC
Tru9I 943 TTAA T^TAA
979 TTAA
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI MaeI MaeII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
SmaI TaqI TseI TspRI TthHB8I
XcmI XhoII XmaCI XmaI