Restriction map for
pFN21AB8937
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AlwNI AocI ApoI AscI AseI
AsnI Asp718I AspI AvrII BalI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstXI BstZ17I Bsu36I CelII
ClaI DraI DraIII DrdI Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FbaI FokI FseI
HincII HindII HindIII HpaI KpnI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvaI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI SacI SalI SbfI ScaI
SexAI SfaNI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 70 GTGCAC G^TGCAC
Aor51HI 701 AGCGCT AGC^GCT
ApaI 412 GGGCCC GGGCC^C
ApaLI 70 GTGCAC G^TGCAC
AspHI 70 GTGCAC G[AT]GC[AT]^C
AviII 710 TGCGCA TGC^GCA
BamHI 197 GGATCC G^GATCC
BbvCI 804 CCTCAGC CCTCAGC(-5/-2)
BfaI 202 CTAG C^TAG
BglI 265 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 391 CTGGAG CTGGAG(16/14)
BseRI 148 GAGGAG GAGGAG(10/8)
BsiHKAI 70 GTGCAC G[AT]GC[AT]^C
BsmI 764 GAATGC GAATGC(1/-1)
BssSI 43 CTCGTG CACGAG(-5/-1)
BstYI 197 GGATCC [GA]^GATC[CT]
CpoI 793 CGGACCG CG^G[AT]CCG
DpnII 198 GATC ^GATC
EarI 181 CTCTTC CTCTTC(1/4)
Eco47III 701 AGCGCT AGC^GCT
FspI 710 TGCGCA TGC^GCA
HphI 577 GGTGA GGTGA(8/7)
Ksp632I 181 CTCTTC CTCTTC(1/4)
MaeI 202 CTAG C^TAG
MboI 198 GATC ^GATC
MflI 197 GGATCC [GA]^GATC[CT]
MslI 571 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 225 GCCGGC GCC^GGC
NdeII 198 GATC ^GATC
NgoMIV 225 GCCGGC G^CCGGC
NotI 340 GCGGCCGC GC^GGCCGC
PaeR7I 478 CTCGAG C^TCGAG
PpuMI 277 AGGACCC [GA]G^G[AT]CC[CT]
RsrII 793 CGGACCG CG^G[AT]CCG
SapI 180 GCTCTTC GCTCTTC(1/4)
Sau3AI 198 GATC ^GATC
SgrAI 224 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 759 CCCGGG CCC^GGG
XhoI 478 CTCGAG C^TCGAG
XhoII 197 GGATCC [GA]^GATC[CT]
XmaCI 759 CCCGGG C^CCGGG
XmaI 759 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 443 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
475 GACCTCGAGTC
AlwI 198 GATCC GGATC(4/5)
197 GGATC
Asp700I 672 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
699 GAAGCGCTTC
AspEI 443 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
475 GACCTCGAGTC
BsmFI 96 GTCCC GGGAC(10/14)
483 GTCCC
BsrFI 225 GCCGGC [GA]^CCGG[CT]
796 ACCGGC
BssHII 625 GCGCGC G^CGCGC
736 GCGCGC
Cfr10I 225 GCCGGC [GA]^CCGG[CT]
796 ACCGGC
EagI 113 CGGCCG C^GGCCG
341 CGGCCG
Eam1105I 443 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
475 GACCTCGAGTC
EclXI 113 CGGCCG C^GGCCG
341 CGGCCG
Eco52I 113 CGGCCG C^GGCCG
341 CGGCCG
HgaI 826 GACGC GACGC(5/10)
143 GCGTC
HinfI 94 GAGTC G^A[ACGT]TC
481 GAGTC
NlaIII 51 CATG CATG^
708 CATG
PleI 94 GAGTC GAGTC(4/5)
481 GAGTC
PstI 594 CTGCAG CTGCA^G
769 CTGCAG
PvuII 597 CAGCTG CAG^CTG
681 CAGCTG
SmlI 478 CTCGAG C^T[CT][GA]AG
550 CTCAAG
XmnI 672 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
699 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsoBI Bsp1286I BsrBI
BssKI BstUI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EcoO109I
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MboII MnlI MspA1I
MspI MvnI MwoI NarI NciI
NlaIV SacII Sau96I ScrFI SfcI
SfoI TaqI TseI TthHB8I