Restriction map for
pFN21AE9570
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccIII AclI AcsI AgeI
AhdI AocI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BglII BlpI Bpu1102I BsaBI
BseAI BsgI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1000 GACGTC GACGT^C
AccI 235 GTCTAC GT^[AC][GT]AC
AcyI 1000 GACGTC G[GA]^CG[CT]C
AflII 693 CTTAAG C^TTAAG
AflIII 104 ACGTGT A^C[GA][CT]GT
AlwI 512 GGATC GGATC(4/5)
Aor51HI 697 AGCGCT AGC^GCT
AvrII 327 CCTAGG C^CTAGG
BalI 929 TGGCCA TGG^CCA
BanI 168 GGTGCC G^G[CT][GA]CC
BfrI 693 CTTAAG C^TTAAG
BlnI 327 CCTAGG C^CTAGG
BsaAI 922 TACGTG [CT]AC^GT[GA]
BsaHI 1000 GACGTC G[GA]^CG[CT]C
BsaI 550 GAGACC GGTCTC(1/5)
BsaWI 659 ACCGGA [AT]^CCGG[AT]
BsiEI 414 CGACCG CG[GA][CT]^CG
BsmFI 1025 GGGAC GGGAC(10/14)
BsrFI 171 GCCGGC [GA]^CCGG[CT]
BsrI 46 CCAGT ACTGG(1/-1)
BstBI 112 TTCGAA TT^CGAA
BstXI 731 CCATCGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 171 GCCGGC [GA]^CCGG[CT]
DpnII 513 GATC ^GATC
Eco47III 697 AGCGCT AGC^GCT
EcoNI 1073 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 944 CCCGC CCCGC(4/6)
Hin1I 1000 GACGTC G[GA]^CG[CT]C
MboI 513 GATC ^GATC
MluNI 929 TGGCCA TGG^CCA
MscI 929 TGGCCA TGG^CCA
MseI 694 TTAA T^TAA
NaeI 171 GCCGGC GCC^GGC
NdeII 513 GATC ^GATC
NgoMIV 171 GCCGGC G^CCGGC
NspI 563 ACATGC [GA]CATG^[CT]
NspV 112 TTCGAA TT^CGAA
PshAI 996 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 513 GATC ^GATC
SexAI 776 ACCTGGT A^CC[AT]GGT
SfuI 112 TTCGAA TT^CGAA
SmlI 693 CTTAAG C^T[CT][GA]AG
StuI 330 AGGCCT AGG^CCT
TaqI 113 TCGA T^CGA
TfiI 962 GAATC G^A[AT]TC
Tru9I 694 TTAA T^TAA
TthHB8I 113 TCGA T^CGA
XcmI 833 CCATGTGCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AluI 263 AGCT AG^CT
1037 AGCT
Alw44I 156 GTGCAC G^TGCAC
1087 GTGCAC
AlwNI 208 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
863 CAGCCTCTG
ApaLI 156 GTGCAC G^TGCAC
1087 GTGCAC
AspHI 156 GTGCAC G[AT]GC[AT]^C
1087 GTGCAC
BbsI 541 GAAGAC GAAGAC(2/6)
993 GAAGAC
BcnI 525 CCCGG CC^[GC]GG
590 CCGGG
BfaI 328 CTAG C^TAG
754 CTAG
BglI 61 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
703 GCCCAGAAGGC
BpmI 1077 CTCCAG CTGGAG(16/14)
442 CTGGAG
BpuAI 541 GAAGAC GAAGAC(2/6)
993 GAAGAC
BsiHKAI 156 GTGCAC G[AT]GC[AT]^C
1087 GTGCAC
BsmAI 550 GAGAC GTCTC(1/5)
821 GAGAC
BstAPI 207 GCAGGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
404 GCATCTCCTGC
BstEII 348 GGTGACC G^GT[ACGT]ACC
1100 GGTCACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
1100 GGTCACC
EarI 391 CTCTTC CTCTTC(1/4)
115 GAAGAG
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
1100 GGTCACC
EcoT14I 327 CCTAGG C^C[AT][AT]GG
713 CCAAGG
HaeII 198 GGCGCT [GA]GCGC^[CT]
697 AGCGCT
Ksp632I 391 CTCTTC CTCTTC(1/4)
115 GAAGAG
MaeI 328 CTAG C^TAG
754 CTAG
MslI 42 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
732 CATCGCGGTG
MspA1I 318 CCGCTG C[AC]G^C[GT]G
797 CCGCTG
NciI 525 CCCGG CC^[GC]GG
590 CCGGG
PleI 447 GAGTC GAGTC(4/5)
1132 GAGTC
StyI 327 CCTAGG C^C[AT][AT]GG
713 CCAAGG
TspRI 47 CAGTG CA[GC]TG[ACGT][ACGT]^
57 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AvaII BbvI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I Bsp1407I BspMI BsrGI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DsaI EaeI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspI MvaI
MvnI MwoI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
SspBI TseI Tsp45I