Restriction map for
pFN21AE4279
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AclI AhdI AocI AseI
AsnI AspEI AvrII BamHI BbeI
BlnI BlpI Bpu1102I BsiWI Bsp1407I
BspDI BspHI BsrGI BssSI Bst1107I
BstZ17I Bsu36I CelII ClaI CpoI
DraIII Eam1105I Eco81I EcoRV HincII
HindII HpaI KasI MfeI MluI
MunI NarI NdeI NheI NotI
NruI PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SfoI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 3226 GACGTC GACGT^C
Acc65I 2042 GGTACC G^GTACC
AccIII 3383 TCCGGA T^CCGGA
AflII 819 CTTAAG C^TTAAG
AflIII 1842 ACATGT A^C[GA][CT]GT
Alw44I 3160 GTGCAC G^TGCAC
Aor51HI 524 AGCGCT AGC^GCT
ApaI 1133 GGGCCC GGGCC^C
ApaLI 3160 GTGCAC G^TGCAC
AscI 79 GGCGCGCC GG^CGCGCC
Asp718I 2042 GGTACC G^GTACC
AspI 2842 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3796 CCCGAG C^[CT]CG[GA]G
AviII 3936 TGCGCA TGC^GCA
BalI 1649 TGGCCA TGG^CCA
BbrPI 3175 CACGTG CAC^GTG
BciVI 2743 GGATAC GTATCC(6/5)
BfrI 819 CTTAAG C^TTAAG
BsaAI 3175 CACGTG [CT]AC^GT[GA]
BsaBI 2968 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3048 GAGACC GGTCTC(1/5)
BseAI 3383 TCCGGA T^CCGGA
BsmI 3581 GAATGC GAATGC(1/-1)
BsoBI 3796 CCCGAG C^[CT]CG[GA]G
BspEI 3383 TCCGGA T^CCGGA
BspLU11I 1842 ACATGT A^CATGT
BsrBI 3123 GAGCGG CCGCTC(-3/-3)
BssHII 80 GCGCGC G^CGCGC
BstAPI 2857 GCACCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 208 TTCGAA TT^CGAA
BstXI 3410 CCAATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2390 GACAGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 524 AGCGCT AGC^GCT
EcoNI 3138 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 194 GGCCGGCC GGCCGG^CC
FspI 3936 TGCGCA TGC^GCA
KpnI 2042 GGTACC GGTAC^C
KspI 155 CCGCGG CCGC^GG
MamI 2968 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1649 TGGCCA TGG^CCA
MroI 3383 TCCGGA T^CCGGA
MscI 1649 TGGCCA TGG^CCA
NaeI 195 GCCGGC GCC^GGC
NcoI 459 CCATGG C^CATGG
NgoMIV 195 GCCGGC G^CCGGC
NspV 208 TTCGAA TT^CGAA
PflFI 2842 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3175 CACGTG CAC^GTG
PmlI 3175 CACGTG CAC^GTG
SacII 155 CCGCGG CCGC^GG
SexAI 638 ACCAGGT A^CC[AT]GGT
SfiI 189 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 208 TTCGAA TT^CGAA
SgrAI 3698 CACCGGTG C[GA]^CCGG[CT]G
Tth111I 2842 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 81 CGCG CG^CG
156 CGCG
AcyI 3226 GACGTC G[GA]^CG[CT]C
3556 GACGCC
AgeI 1035 ACCGGT A^CCGGT
3699 ACCGGT
AlwNI 1301 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1432 CAGAGTCTG
BbvCI 3681 CCTCAGC CCTCAGC(-5/-2)
2926 GCTGAGG
BcgI 1637 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3686 GCATGTTATTCG
BclI 1557 TGATCA T^GATCA
3720 TGATCA
BglII 2688 AGATCT A^GATCT
2967 AGATCT
BsaHI 3226 GACGTC G[GA]^CG[CT]C
3556 GACGCC
BsmBI 2583 CGTCTC CGTCTC(1/5)
3224 GAGACG
BspMI 1239 GCAGGT ACCTGC(4/8)
2233 GCAGGT
BsrDI 3951 CATTGC GCAATG(2/0)
1909 GCAATG
BstEII 57 GGTGACC G^GT[ACGT]ACC
549 GGTCACC
BstPI 57 GGTGACC G^GT[ACGT]ACC
549 GGTCACC
BstUI 81 CGCG CG^CG
156 CGCG
DraI 2002 TTTAAA TTT^AAA
2470 TTTAAA
EagI 193 CGGCCG C^GGCCG
197 CGGCCG
EclXI 193 CGGCCG C^GGCCG
197 CGGCCG
Eco52I 193 CGGCCG C^GGCCG
197 CGGCCG
EcoO65I 57 GGTGACC G^GT[ACGT]ACC
549 GGTCACC
EcoRI 739 GAATTC G^AATTC
2443 GAATTC
EcoT22I 1201 ATGCAT ATGCA^T
2155 ATGCAT
FbaI 1557 TGATCA T^GATCA
3720 TGATCA
Hin1I 3226 GACGTC G[GA]^CG[CT]C
3556 GACGCC
HindIII 2782 AAGCTT A^AGCTT
3964 AAGCTT
MvnI 81 CGCG CG^CG
156 CGCG
NsiI 1201 ATGCAT ATGCA^T
2155 ATGCAT
PinAI 1035 ACCGGT A^CCGGT
3699 ACCGGT
PpuMI 2309 AGGACCC [GA]G^G[AT]CC[CT]
2671 AGGTCCC
PshAI 1493 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2319 GACAACTGTC
PvuII 870 CAGCTG CAG^CTG
1464 CAGCTG
ScaI 3191 AGTACT AGT^ACT
3734 AGTACT
SspI 1336 AATATT AAT^ATT
3859 AATATT
XbaI 1501 TCTAGA T^CTAGA
1857 TCTAGA
XcmI 303 CCAACCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
725 CCAAGAGTTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI