Restriction map for
pFN21AE4365
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BfaI BfrI
BglII BlnI BpmI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 547 GGCGTC G[GA]^CG[CT]C
Alw44I 814 GTGCAC G^TGCAC
ApaI 385 GGGCCC GGGCC^C
ApaLI 814 GTGCAC G^TGCAC
AspI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 509 TGGCCA TGG^CCA
BbsI 973 GTCTTC GAAGAC(2/6)
BcgI 554 GCACTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 395 TGATCA T^GATCA
BlpI 747 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 747 GCTGAGC GC^T[ACGT]AGC
BpuAI 973 GTCTTC GAAGAC(2/6)
BsaHI 547 GGCGTC G[GA]^CG[CT]C
BsiEI 8 CGACCG CG[GA][CT]^CG
BsrI 165 CCAGT ACTGG(1/-1)
BssSI 817 CACGAG CACGAG(-5/-1)
BstXI 941 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 747 GCTGAGC GC^T[ACGT]AGC
EcoNI 894 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1064 GCGGG CCCGC(4/6)
FbaI 395 TGATCA T^GATCA
Hin1I 547 GGCGTC G[GA]^CG[CT]C
HincII 1081 GTCAAC GT[CT]^[GA]AC
HindII 1081 GTCAAC GT[CT]^[GA]AC
MluNI 509 TGGCCA TGG^CCA
MscI 509 TGGCCA TGG^CCA
NspI 1022 ACATGC [GA]CATG^[CT]
PflFI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 197 GACTC GAGTC(4/5)
PvuII 516 CAGCTG CAG^CTG
SfaNI 848 GCATC GCATC(5/9)
TfiI 104 GATTC G^A[AT]TC
Tsp509I 493 AATT ^AATT
Tth111I 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 54 CCACAGCACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 354 GTAC GT^AC
1056 GTAC
BsaI 991 GAGACC GGTCTC(1/5)
721 GGTCTC
BsmAI 991 GAGAC GTCTC(1/5)
722 GTCTC
BsmI 73 GAATGC GAATGC(1/-1)
334 GAATGC
BssHII 741 GCGCGC G^CGCGC
743 GCGCGC
BstEII 624 GGTGACC G^GT[ACGT]ACC
762 GGTCACC
BstPI 624 GGTGACC G^GT[ACGT]ACC
762 GGTCACC
DdeI 486 CTCAG C^T[ACGT]AG
748 CTGAG
DrdI 993 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1072 GACCTGGCCGTC
EcoO65I 624 GGTGACC G^GT[ACGT]ACC
762 GGTCACC
HgaI 26 GCGTC GACGC(5/10)
548 GCGTC
HinfI 104 GATTC G^A[ACGT]TC
197 GACTC
MslI 426 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
678 CACCCAGGTG
NaeI 592 GCCGGC GCC^GGC
1132 GCCGGC
NgoMIV 592 GCCGGC G^CCGGC
1132 GCCGGC
PstI 612 CTGCAG CTGCA^G
670 CTGCAG
RsaI 354 GTAC GT^AC
1056 GTAC
SacI 247 GAGCTC GAGCT^C
871 GAGCTC
SfcI 612 CTGCAG C^T[GA][CT]AG
670 CTGCAG
SmaI 211 CCCGGG CCC^GGG
349 CCCGGG
TaqI 602 TCGA T^CGA
1106 TCGA
TthHB8I 602 TCGA T^CGA
1106 TCGA
XmaCI 211 CCCGGG C^CCGGG
349 CCCGGG
XmaI 211 CCCGGG C^CCGGG
349 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI