Restriction map for
pFN21AE4391
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 910 GTAGAC GT^[AC][GT]AC
AciI 1103 CCGC CCGC(-3/-1)
AcsI 504 GAATTT [GA]^AATT[CT]
AflIII 914 ACGTGT A^C[GA][CT]GT
Alw44I 1057 GTGCAC G^TGCAC
AlwI 655 GATCC GGATC(4/5)
ApaLI 1057 GTGCAC G^TGCAC
ApoI 504 GAATTT [GA]^AATT[CT]
Asp700I 210 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 20 TGGCCA TGG^CCA
BcnI 592 CCCGG CC^[GC]GG
BfaI 13 CTAG C^TAG
BglII 330 AGATCT A^GATCT
BlpI 1066 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1066 GCTGAGC GC^T[ACGT]AGC
BsaWI 1111 ACCGGA [AT]^CCGG[AT]
BseRI 40 CTCCTC GAGGAG(10/8)
BspHI 336 TCATGA T^CATGA
BspMI 861 GCAGGT ACCTGC(4/8)
BssSI 361 CACGAG CACGAG(-5/-1)
BstXI 23 CCATTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 330 AGATCT [GA]^GATC[CT]
CelII 1066 GCTGAGC GC^T[ACGT]AGC
DraII 383 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 20 TGGCCA [CT]^GGCC[GA]
EarI 1207 CTCTTC CTCTTC(1/4)
EcoO109I 383 AGGACCT [GA]G^G[ACGT]CC[CT]
HincII 1225 GTTAAC GT[CT]^[GA]AC
HindII 1225 GTTAAC GT[CT]^[GA]AC
HindIII 92 AAGCTT A^AGCTT
HpaI 1225 GTTAAC GTT^AAC
Ksp632I 1207 CTCTTC CTCTTC(1/4)
MaeI 13 CTAG C^TAG
MaeII 914 ACGT A^CGT
MflI 330 AGATCT [GA]^GATC[CT]
MluNI 20 TGGCCA TGG^CCA
MscI 20 TGGCCA TGG^CCA
MslI 597 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1103 CCGCTG C[AC]G^C[GT]G
NciI 592 CCCGG CC^[GC]GG
PpuMI 383 AGGACCT [GA]G^G[AT]CC[CT]
PstI 430 CTGCAG CTGCA^G
RcaI 336 TCATGA T^CATGA
SapI 1206 GCTCTTC GCTCTTC(1/4)
StuI 15 AGGCCT AGG^CCT
Tsp45I 897 GTCAC ^GT[GC]AC
XhoII 330 AGATCT [GA]^GATC[CT]
XmnI 210 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 922 GTAC GT^AC
1091 GTAC
AlwNI 424 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1120 CAGACTCTG
AvaII 105 GGTCC G^G[AT]CC
384 GGACC
BpmI 876 CTCCAG CTGGAG(16/14)
688 CTGGAG
BsmAI 1135 GAGAC GTCTC(1/5)
982 GTCTC
DsaI 555 CCATGG C^C[GA][CT]GG
1130 CCATGG
HapII 593 CCGG C^CGG
1112 CCGG
HpaII 593 CCGG C^CGG
1112 CCGG
MaeIII 664 GTAAC ^GT[ACGT]AC
897 GTCAC
MspI 593 CCGG C^CGG
1112 CCGG
NcoI 555 CCATGG C^CATGG
1130 CCATGG
RsaI 922 GTAC GT^AC
1091 GTAC
SfcI 239 CTACAG C^T[GA][CT]AG
430 CTGCAG
SmlI 178 CTCAAG C^T[CT][GA]AG
228 CTTGAG
TspRI 474 CAGTG CA[GC]TG[ACGT][ACGT]^
597 CACTG
XcmI 8 CCACACTAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
251 CCATGCTGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AspHI BanI BbsI BbvI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp509I TthHB8I