Restriction map for
pFN21AE3073
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AluI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbsI
BbvCI BclI BcnI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaHI BsaI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MroI MseI MslI MspA1I
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 229 CGCG CG^CG
AocI 625 CCTGAGG CC^T[ACGT]AGG
AspHI 274 GAGCAC G[AT]GC[AT]^C
BalI 512 TGGCCA TGG^CCA
BanII 505 GAGCCC G[GA]GC[CT]^C
BbrPI 616 CACGTG CAC^GTG
BcgI 369 CGACCAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 597 GGATAC GTATCC(6/5)
BglII 209 AGATCT A^GATCT
BpmI 181 CTGGAG CTGGAG(16/14)
BsaBI 471 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 83 ACCGGA [AT]^CCGG[AT]
BsiHKAI 274 GAGCAC G[AT]GC[AT]^C
BspMI 650 ACCTGC ACCTGC(4/8)
BsrDI 564 CATTGC GCAATG(2/0)
BsrI 321 ACTGG ACTGG(1/-1)
BssKI 180 CCTGG ^CC[ACGT]GG
BstAPI 396 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 180 CCTGG CC^[AT]GG
BstUI 229 CGCG CG^CG
BstYI 209 AGATCT [GA]^GATC[CT]
Bsu36I 625 CCTGAGG CC^T[ACGT]AGG
CfoI 405 GCGC GCG^C
EaeI 512 TGGCCA [CT]^GGCC[GA]
EarI 198 GAAGAG CTCTTC(1/4)
Eco81I 625 CCTGAGG CC^T[ACGT]AGG
EcoRII 180 CCTGG ^CC[AT]GG
HhaI 405 GCGC GCG^C
HinP1I 405 GCGC G^CGC
HinfI 184 GAGTC G^A[ACGT]TC
HphI 116 TCACC GGTGA(8/7)
Ksp632I 198 GAAGAG CTCTTC(1/4)
MamI 471 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 209 AGATCT [GA]^GATC[CT]
MluI 228 ACGCGT A^CGCGT
MluNI 512 TGGCCA TGG^CCA
MscI 512 TGGCCA TGG^CCA
MvaI 180 CCTGG CC^[AT]GG
MvnI 229 CGCG CG^CG
MwoI 396 GCAGTTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 509 CCATGG C^CATGG
NlaIII 510 CATG CATG^
PleI 184 GAGTC GAGTC(4/5)
PmaCI 616 CACGTG CAC^GTG
PmlI 616 CACGTG CAC^GTG
PstI 235 CTGCAG CTGCA^G
SapI 198 GAAGAGC GCTCTTC(1/4)
ScrFI 180 CCTGG CC^[ACGT]GG
TaqI 95 TCGA T^CGA
TthHB8I 95 TCGA T^CGA
XhoII 209 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 232 GTAC GT^AC
582 GTAC
AflIII 228 ACGCGT A^C[GA][CT]GT
617 ACGTGT
AlwNI 397 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
553 CAGGCCCTG
Asp700I 147 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
357 GAAGGTGTTC
AvaII 60 GGACC G^G[AT]CC
384 GGACC
BbvI 688 GCAGC GCAGC(8/12)
259 GCTGC
BmyI 274 GAGCAC G[AGT]GC[ACT]^C
505 GAGCCC
BsaAI 583 TACGTG [CT]AC^GT[GA]
616 CACGTG
BseRI 271 GAGGAG GAGGAG(10/8)
466 GAGGAG
BsgI 489 CTGCAC GTGCAG(16/14)
394 GTGCAG
BsmFI 59 GGGAC GGGAC(10/14)
186 GTCCC
Bsp1286I 274 GAGCAC G[AGT]GC[ACT]^C
505 GAGCCC
DpnII 210 GATC ^GATC
537 GATC
DraII 160 AGGGCCT [GA]G^G[ACGT]CC[CT]
554 AGGCCCT
DsaI 509 CCATGG C^C[GA][CT]GG
515 CCACGG
EcoNI 351 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
422 CCTTCATCAGG
EcoO109I 160 AGGGCCT [GA]G^G[ACGT]CC[CT]
554 AGGCCCT
EcoT14I 164 CCTTGG C^C[AT][AT]GG
509 CCATGG
HapII 84 CCGG C^CGG
450 CCGG
HpaII 84 CCGG C^CGG
450 CCGG
MaeIII 38 GTGAC ^GT[ACGT]AC
524 GTGAC
MboI 210 GATC ^GATC
537 GATC
MspI 84 CCGG C^CGG
450 CCGG
NdeII 210 GATC ^GATC
537 GATC
NlaIV 59 GGGACC GG[ACGT]^[ACGT]CC
431 GGAACC
RsaI 232 GTAC GT^AC
582 GTAC
Sau3AI 210 GATC ^GATC
537 GATC
SfcI 156 CTACAG C^T[GA][CT]AG
235 CTGCAG
StyI 164 CCTTGG C^C[AT][AT]GG
509 CCATGG
TseI 259 GCTGC G^C[AT]GC
688 GCAGC
Tsp45I 38 GTGAC ^GT[GC]AC
524 GTGAC
TspRI 309 CAGTG CA[GC]TG[ACGT][ACGT]^
341 CAGTG
XmnI 147 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
357 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BstF5I
Cac8I Cfr13I DdeI DraIII Fnu4HI
FokI HaeIII ItaI MaeII MboII
MnlI Sau96I