##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     MaeII       MaeIII      
MamI        MboI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvaI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacI        SalI        
SapI        Sau3AI      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AluI          174  AGCT                 AG^CT
ApaI           94  GGGCCC               GGGCC^C
Asp700I       198  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           90  CTCGGG               C^[CT]CG[GA]G
BanII          94  GGGCCC               G[GA]GC[CT]^C
BfaI          187  CTAG                 C^TAG
BmyI           94  GGGCCC               G[AGT]GC[ACT]^C
BsaJI         122  CCGCGG               C^C[ACGT][ACGT]GG
BsoBI          90  CTCGGG               C^[CT]CG[GA]G
Bsp1286I       94  GGGCCC               G[AGT]GC[ACT]^C
BsrBI          71  CCGCTC               CCGCTC(-3/-3)
DsaI          122  CCGCGG               C^C[GA][CT]GG
HaeII          17  GGCGCT               [GA]GCGC^[CT]
KspI          122  CCGCGG               CCGC^GG
MaeI          187  CTAG                 C^TAG
MnlI            4  CCTC                 CCTC(7/6)
MseI          166  TTAA                 T^TAA
NdeI          179  CATATG               CA^TATG
PstI           64  CTGCAG               CTGCA^G
PvuII         173  CAGCTG               CAG^CTG
SacII         122  CCGCGG               CCGC^GG
SfcI           64  CTGCAG               C^T[GA][CT]AG
Tru9I         166  TTAA                 T^TAA
XmnI          198  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccII         101  CGCG                 CG^CG
              123  CGCG                
BbvI           66  GCAGC                GCAGC(8/12)
              175  GCTGC               
BsiYI          42  CCGCCGCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               86  CCCACTCGGGG         
BslI           42  CCGCCGCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               86  CCCACTCGGGG         
BstUI         101  CGCG                 CG^CG
              123  CGCG                
CfoI           18  GCGC                 GCG^C
              102  GCGC                
DraII          93  GGGGCCC              [GA]G^G[ACGT]CC[CT]
               94  GGGCCCC             
EcoO109I       93  GGGGCCC              [GA]G^G[ACGT]CC[CT]
               94  GGGCCCC             
HhaI           18  GCGC                 GCG^C
              102  GCGC                
HinP1I         18  GCGC                 G^CGC
              102  GCGC                
MvnI          101  CGCG                 CG^CG
              123  CGCG                
TseI           66  GCAGC                G^C[AT]GC
              175  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        Cac8I       Cfr13I      FauI        Fnu4HI      
HaeIII      ItaI        MspA1I      MwoI        NlaIV       
Sau96I