Restriction map for
pFN21AE1143
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# NO CUT ENZYMES ( 131 enzymes) #
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Acc65I AccII AccIII AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BfrI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI HindIII HpaI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 64 GACGTC GACGT^C
AccI 61 GTCGAC GT^[AC][GT]AC
AclI 560 AACGTT AA^CGTT
Asp700I 1219 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 135 GTGCTC G[AT]GC[AT]^C
BbeI 16 GGCGCC GGCGC^C
BciVI 1074 GGATAC GTATCC(6/5)
BclI 657 TGATCA T^GATCA
BcnI 744 CCGGG CC^[GC]GG
BglII 972 AGATCT A^GATCT
BlpI 877 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 877 GCTAAGC GC^T[ACGT]AGC
BsaI 414 GAGACC GGTCTC(1/5)
BsiHKAI 135 GTGCTC G[AT]GC[AT]^C
BsmI 328 GAATGC GAATGC(1/-1)
BsrFI 781 GCCGGC [GA]^CCGG[CT]
BstAPI 169 GCACTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 972 AGATCT [GA]^GATC[CT]
CelII 877 GCTAAGC GC^T[ACGT]AGC
Cfr10I 781 GCCGGC [GA]^CCGG[CT]
Cfr13I 914 GGCCC G^G[ACGT]CC
DraI 316 TTTAAA TTT^AAA
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoT14I 486 CCATGG C^C[AT][AT]GG
FbaI 657 TGATCA T^GATCA
HaeII 16 GGCGCC [GA]GCGC^[CT]
HincII 61 GTCGAC GT[CT]^[GA]AC
HindII 61 GTCGAC GT[CT]^[GA]AC
HphI 655 GGTGA GGTGA(8/7)
KasI 16 GGCGCC G^GCGCC
MfeI 592 CAATTG C^AATTG
MflI 972 AGATCT [GA]^GATC[CT]
MslI 496 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 20 CCGCTG C[AC]G^C[GT]G
MunI 592 CAATTG C^AATTG
NaeI 781 GCCGGC GCC^GGC
NarI 16 GGCGCC GG^CGCC
NciI 744 CCGGG CC^[GC]GG
NcoI 486 CCATGG C^CATGG
NgoMIV 781 GCCGGC G^CCGGC
Psp1406I 560 AACGTT AA^CGTT
PstI 1269 CTGCAG CTGCA^G
SalI 61 GTCGAC G^TCGAC
Sau96I 914 GGCCC G^G[ACGT]CC
SfoI 16 GGCGCC GGC^GCC
StuI 548 AGGCCT AGG^CCT
StyI 486 CCATGG C^C[AT][AT]GG
TaqI 62 TCGA T^CGA
Tsp45I 254 GTCAC ^GT[GC]AC
TthHB8I 62 TCGA T^CGA
XbaI 975 TCTAGA T^CTAGA
XhoII 972 AGATCT [GA]^GATC[CT]
XmnI 1219 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 16 GGCGCC G[GA]^CG[CT]C
64 GACGTC
BanI 16 GGCGCC G^G[CT][GA]CC
502 GGTGCC
BanII 43 GAGCCC G[GA]GC[CT]^C
150 GGGCTC
BfaI 788 CTAG C^TAG
976 CTAG
BsaHI 16 GGCGCC G[GA]^CG[CT]C
64 GACGTC
BseRI 73 GAGGAG GAGGAG(10/8)
82 GAGGAG
BsgI 189 CTGCAC GTGCAG(16/14)
587 CTGCAC
BsmAI 162 GAGAC GTCTC(1/5)
414 GAGAC
BspMI 187 ACCTGC ACCTGC(4/8)
943 ACCTGC
CfoI 17 GCGC GCG^C
104 GCGC
DsaI 47 CCGTGG C^C[GA][CT]GG
486 CCATGG
EarI 1219 GAAGAG CTCTTC(1/4)
1244 GAAGAG
EcoNI 69 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
705 CCTCAAAGAGG
HhaI 17 GCGC GCG^C
104 GCGC
Hin1I 16 GGCGCC G[GA]^CG[CT]C
64 GACGTC
HinP1I 17 GCGC G^CGC
104 GCGC
Ksp632I 1219 GAAGAG CTCTTC(1/4)
1244 GAAGAG
MaeI 788 CTAG C^TAG
976 CTAG
MaeII 65 ACGT A^CGT
561 ACGT
MaeIII 254 GTCAC ^GT[ACGT]AC
601 GTAAC
PflMI 905 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCAGCAAGTGG
PleI 124 GACTC GAGTC(4/5)
1097 GAGTC
SfcI 180 CTGTAG C^T[GA][CT]AG
1269 CTGCAG
SmlI 70 CTTGAG C^T[CT][GA]AG
1159 CTCAAG
Van91I 905 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCAGCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI