##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       ClaI        CpoI        DpnII       DraI        
DraII       DraIII      DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII          17  CGCG                 CG^CG
AcsI           74  GAATTC               [GA]^AATT[CT]
AcyI           90  GGCGTC               G[GA]^CG[CT]C
AluI          101  AGCT                 AG^CT
ApoI           74  GAATTC               [GA]^AATT[CT]
Asp700I       211  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         264  GGTCC                G^G[AT]CC
BbvCI           8  CCTCAGC              CCTCAGC(-5/-2)
BfaI          278  CTAG                 C^TAG
BsaHI          90  GGCGTC               G[GA]^CG[CT]C
BsaJI          79  CCTTGG               C^C[ACGT][ACGT]GG
BsmAI         163  GAGAC                GTCTC(1/5)
BsmI          222  GAATGC               GAATGC(1/-1)
BsrFI          54  GCCGGT               [GA]^CCGG[CT]
BsrI          166  ACTGG                ACTGG(1/-1)
BssKI         267  CCAGG                ^CC[ACGT]GG
BstNI         267  CCAGG                CC^[AT]GG
BstUI          17  CGCG                 CG^CG
Cfr10I         54  GCCGGT               [GA]^CCGG[CT]
Cfr13I        264  GGTCC                G^G[ACGT]CC
DdeI            9  CTCAG                C^T[ACGT]AG
DrdI           84  GACTGCGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          126  CGGCCA               [CT]^GGCC[GA]
EcoRI          74  GAATTC               G^AATTC
EcoRII        267  CCAGG                ^CC[AT]GG
EcoT14I        79  CCTTGG               C^C[AT][AT]GG
HaeII          30  GGCGCT               [GA]GCGC^[CT]
HapII          55  CCGG                 C^CGG
HgaI           91  GCGTC                GACGC(5/10)
Hin1I          90  GGCGTC               G[GA]^CG[CT]C
HinfI          96  GAGTC                G^A[ACGT]TC
HpaII          55  CCGG                 C^CGG
MaeI          278  CTAG                 C^TAG
MspA1I        100  CAGCTG               C[AC]G^C[GT]G
MspI           55  CCGG                 C^CGG
MvaI          267  CCAGG                CC^[AT]GG
MvnI           17  CGCG                 CG^CG
NlaIII        130  CATG                 CATG^
PleI           96  GAGTC                GAGTC(4/5)
PvuII         100  CAGCTG               CAG^CTG
Sau96I        264  GGTCC                G^G[ACGT]CC
ScrFI         267  CCAGG                CC^[ACGT]GG
StyI           79  CCTTGG               C^C[AT][AT]GG
TaqI           94  TCGA                 T^CGA
Tsp509I        75  AATT                 ^AATT
TthHB8I        94  TCGA                 T^CGA
XmnI          211  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

CfoI           16  GCGC                 GCG^C
               31  GCGC                
HhaI           16  GCGC                 GCG^C
               31  GCGC                
HinP1I         16  GCGC                 G^CGC
               31  GCGC                


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AciI        BbvI        Fnu4HI      HaeIII      ItaI        
MboII       MnlI        MwoI        TseI