Restriction map for
pFN21AE4436
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BcnI BfrI BglII BlnI
BpmI BsaBI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT14I FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MfeI MluI MroI MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 1014 GAATTT [GA]^AATT[CT]
AflIII 730 ACATGT A^C[GA][CT]GT
AgeI 555 ACCGGT A^CCGGT
ApoI 1014 GAATTT [GA]^AATT[CT]
AvaI 26 CTCGGG C^[CT]CG[GA]G
BalI 962 TGGCCA TGG^CCA
BamHI 1332 GGATCC G^GATCC
BanII 788 GGGCTC G[GA]GC[CT]^C
BbsI 160 GTCTTC GAAGAC(2/6)
BbvCI 860 CCTCAGC CCTCAGC(-5/-2)
BclI 1266 TGATCA T^GATCA
BglI 457 GCCAAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 160 GTCTTC GAAGAC(2/6)
BsaAI 1249 TACGTG [CT]AC^GT[GA]
BsaI 99 GGTCTC GGTCTC(1/5)
BsaWI 555 ACCGGT [AT]^CCGG[AT]
BseRI 1345 CTCCTC GAGGAG(10/8)
BsgI 337 GTGCAG GTGCAG(16/14)
BsmBI 81 GAGACG CGTCTC(1/5)
BsoBI 26 CTCGGG C^[CT]CG[GA]G
BspLU11I 730 ACATGT A^CATGT
BspMI 339 GCAGGT ACCTGC(4/8)
BsrBI 39 CCGCTC CCGCTC(-3/-3)
BstEII 121 GGTTACC G^GT[ACGT]ACC
BstPI 121 GGTTACC G^GT[ACGT]ACC
BstXI 917 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 696 GCGC GCG^C
DsaI 970 CCACGG C^C[GA][CT]GG
EaeI 962 TGGCCA [CT]^GGCC[GA]
EcoO65I 121 GGTTACC G^GT[ACGT]ACC
EcoT22I 1186 ATGCAT ATGCA^T
FauI 14 CCCGC CCCGC(4/6)
FbaI 1266 TGATCA T^GATCA
HgaI 708 GCGTC GACGC(5/10)
HhaI 696 GCGC GCG^C
HinP1I 696 GCGC G^CGC
MluNI 962 TGGCCA TGG^CCA
MscI 962 TGGCCA TGG^CCA
MslI 543 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 462 GCCGGC GCC^GGC
NgoMIV 462 GCCGGC G^CCGGC
NsiI 1186 ATGCAT ATGCA^T
NspI 730 ACATGT [GA]CATG^[CT]
PinAI 555 ACCGGT A^CCGGT
PleI 1340 GAGTC GAGTC(4/5)
PvuII 188 CAGCTG CAG^CTG
SexAI 117 ACCAGGT A^CC[AT]GGT
SgrAI 554 CACCGGTG C[GA]^CCGG[CT]G
StuI 857 AGGCCT AGG^CCT
TaqI 401 TCGA T^CGA
TfiI 238 GAATC G^A[AT]TC
Tsp509I 1015 AATT ^AATT
TthHB8I 401 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 381 GGTACC G^GTACC
1099 GGTACC
Asp718I 381 GGTACC G^GTACC
1099 GGTACC
AvaII 115 GGACC G^G[AT]CC
1034 GGACC
BlpI 631 GCTGAGC GC^T[ACGT]AGC
703 GCTCAGC
BmyI 559 GTGCCC G[AGT]GC[ACT]^C
788 GGGCTC
Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC
703 GCTCAGC
BsmFI 114 GGGAC GGGAC(10/14)
6 GTCCC
Bsp1286I 559 GTGCCC G[AGT]GC[ACT]^C
788 GGGCTC
BstYI 1332 GGATCC [GA]^GATC[CT]
1353 GGATCT
CelII 631 GCTGAGC GC^T[ACGT]AGC
703 GCTCAGC
HinfI 238 GAATC G^A[ACGT]TC
1340 GAGTC
KpnI 381 GGTACC GGTAC^C
1099 GGTACC
MaeII 84 ACGT A^CGT
1250 ACGT
MflI 1332 GGATCC [GA]^GATC[CT]
1353 GGATCT
MseI 149 TTAA T^TAA
752 TTAA
PflMI 352 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
637 CCAGGAGCTGG
Tru9I 149 TTAA T^TAA
752 TTAA
Tsp45I 244 GTCAC ^GT[GC]AC
1297 GTGAC
Van91I 352 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
637 CCAGGAGCTGG
XhoII 1332 GGATCC [GA]^GATC[CT]
1353 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanI BbvI BfaI BsaJI BsiYI
BslI BsmAI BsrDI BsrFI BsrI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI XcmI